The Salmonella effector protein SpvC, a phosphothreonine lyase is functional in plant cells

Handle URI:
http://hdl.handle.net/10754/555860
Title:
The Salmonella effector protein SpvC, a phosphothreonine lyase is functional in plant cells
Authors:
Neumann, Christina; Fraiture, Malou; Hernàndez-Reyes, Casandra; Akum, Fidele N.; Virlogeux-Payant, Isabelle; Chen, Ying; Pateyron, Stephanie; Colcombet, Jean; Kogel, Karl-Heinz; Hirt, Heribert ( 0000-0003-3119-9633 ) ; Brunner, Frédéric; Schikora, Adam
Abstract:
Salmonella is one of the most prominent causes of food poisoning and growing evidence indicates that contaminated fruits and vegetables are an increasing concern for human health. Successful infection demands the suppression of the host immune system, which is often achieved via injection of bacterial effector proteins into host cells. In this report we present the function of Salmonella effector protein in plant cell, supporting the new concept of trans-kingdom competence of this bacterium. We screened a range of Salmonella Typhimurium effector proteins for interference with plant immunity. Among these, the phosphothreonine lyase SpvC attenuated the induction of immunity-related genes when present in plant cells. Using in vitro and in vivo systems we show that this effector protein interacts with and dephosphorylates activated Arabidopsis Mitogen-activated Protein Kinase 6 (MPK6), thereby inhibiting defense signaling. Moreover, the requirement of Salmonella SpvC was shown by the decreased proliferation of the ΔspvC mutant in Arabidopsis plants. These results suggest that some Salmonella effector proteins could have a conserved function during proliferation in different hosts. The fact that Salmonella and other Enterobacteriaceae use plants as hosts strongly suggests that plants represent a much larger reservoir for animal pathogens than so far estimated.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Center for Desert Agriculture
Citation:
The Salmonella effector protein SpvC, a phosphothreonine lyase is functional in plant cells 2014, 5 Frontiers in Microbiology
Publisher:
Frontiers Media S.A.
Journal:
Frontiers in Microbiology
Issue Date:
17-Oct-2014
DOI:
10.3389/fmicb.2014.00548
Type:
Article
ISSN:
1664-302X
Additional Links:
http://journal.frontiersin.org/article/10.3389/fmicb.2014.00548/abstract
Appears in Collections:
Articles; Center for Desert Agriculture; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorNeumann, Christinaen
dc.contributor.authorFraiture, Malouen
dc.contributor.authorHernàndez-Reyes, Casandraen
dc.contributor.authorAkum, Fidele N.en
dc.contributor.authorVirlogeux-Payant, Isabelleen
dc.contributor.authorChen, Yingen
dc.contributor.authorPateyron, Stephanieen
dc.contributor.authorColcombet, Jeanen
dc.contributor.authorKogel, Karl-Heinzen
dc.contributor.authorHirt, Heriberten
dc.contributor.authorBrunner, Frédéricen
dc.contributor.authorSchikora, Adamen
dc.date.accessioned2015-05-27T07:35:41Zen
dc.date.available2015-05-27T07:35:41Zen
dc.date.issued2014-10-17en
dc.identifier.citationThe Salmonella effector protein SpvC, a phosphothreonine lyase is functional in plant cells 2014, 5 Frontiers in Microbiologyen
dc.identifier.issn1664-302Xen
dc.identifier.doi10.3389/fmicb.2014.00548en
dc.identifier.urihttp://hdl.handle.net/10754/555860en
dc.description.abstractSalmonella is one of the most prominent causes of food poisoning and growing evidence indicates that contaminated fruits and vegetables are an increasing concern for human health. Successful infection demands the suppression of the host immune system, which is often achieved via injection of bacterial effector proteins into host cells. In this report we present the function of Salmonella effector protein in plant cell, supporting the new concept of trans-kingdom competence of this bacterium. We screened a range of Salmonella Typhimurium effector proteins for interference with plant immunity. Among these, the phosphothreonine lyase SpvC attenuated the induction of immunity-related genes when present in plant cells. Using in vitro and in vivo systems we show that this effector protein interacts with and dephosphorylates activated Arabidopsis Mitogen-activated Protein Kinase 6 (MPK6), thereby inhibiting defense signaling. Moreover, the requirement of Salmonella SpvC was shown by the decreased proliferation of the ΔspvC mutant in Arabidopsis plants. These results suggest that some Salmonella effector proteins could have a conserved function during proliferation in different hosts. The fact that Salmonella and other Enterobacteriaceae use plants as hosts strongly suggests that plants represent a much larger reservoir for animal pathogens than so far estimated.en
dc.publisherFrontiers Media S.A.en
dc.relation.urlhttp://journal.frontiersin.org/article/10.3389/fmicb.2014.00548/abstracten
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.subjectplant infectionen
dc.subjectSalmonellaen
dc.subjecttrans-kingdom pathogenicityen
dc.subjectT3SSen
dc.titleThe Salmonella effector protein SpvC, a phosphothreonine lyase is functional in plant cellsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalFrontiers in Microbiologyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionResearch Center for BioSystems, Land Use and Nutrition, Institute for Phytopathology and Applied Zoology, Justus-Liebig University Giessen, Giessen, Germanyen
dc.contributor.institutionDepartment of Plant Biochemistry, Center for Plant Molecular Biology (ZMBP), Eberhard Karls University Tübingen, Tübingen, Germanyen
dc.contributor.institutionInstitut National de la Recherche Agronomique, UMR1282 Infectiologie et Santé Publique, Nouzilly, Franceen
dc.contributor.institutionUniversité François Rabelais de Tours, UMR1282 Infectiologie et Santé Publique, Tours, Franceen
dc.contributor.institutionUnité de Recherche en Génomique Végétale, Plant Genomics, Evry, Franceen
kaust.authorHirt, Heriberten
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