The regulatory role of the NO/cGMP signal transduction cascade during larval attachment and metamorphosis of the barnacle Balanus (=Amphibalanus) amphitrite

Handle URI:
http://hdl.handle.net/10754/555802
Title:
The regulatory role of the NO/cGMP signal transduction cascade during larval attachment and metamorphosis of the barnacle Balanus (=Amphibalanus) amphitrite
Authors:
Zhang, Y.; He, L.-S.; Zhang, G.; Xu, Y.; Lee, O.-O.; Matsumura, K.; Qian, P.-Y.
Abstract:
The barnacle Balanus amphitrite is among the most dominant fouling species on intertidal rocky shores in tropical and subtropical areas and is thus a target organism in antifouling research. After being released from adults, the swimming nauplius undertakes six molting cycles and then transforms into a cyprid. Using paired antennules, a competent cyprid actively explores and selects a suitable substratum for attachment and metamorphosis (collectively known as settlement). This selection process involves the reception of exogenous signals and subsequent endogenous signal transduction. To investigate the involvement of nitric oxide (NO) and cyclic GMP (cGMP) during larval settlement of B. amphitrite, we examined the effects of an NO donor and an NO scavenger, two nitric oxide synthase (NOS) inhibitors and a soluble guanylyl cyclase (sGC) inhibitor on settling cyprids. We found that the NO donor sodium nitroprusside (SNP) inhibited larval settlement in a dose-dependent manner. In contrast, both the NO scavenger carboxy-PTIO and the NOS inhibitors aminoguanidine hemisulfate (AGH) and S-methylisothiourea sulfate (SMIS) significantly accelerated larval settlement. Suppression of the downstream guanylyl cyclase (GC) activity using a GC-selective inhibitor ODQ could also significantly accelerate larval settlement. Interestingly, the settlement inhibition effects of SNP could be attenuated by ODQ at all concentrations tested. In the developmental expression profiling of NOS and sGC, the lowest expression of both genes was detected in the cyprid stage, a crucial stage for the larval decision to attach and metamorphose. In summary, we concluded that NO regulates larval settlement via mediating downstream cGMP signaling.
Citation:
The regulatory role of the NO/cGMP signal transduction cascade during larval attachment and metamorphosis of the barnacle Balanus (=Amphibalanus) amphitrite 2012, 215 (21):3813 Journal of Experimental Biology
Publisher:
The Company of Biologists
Journal:
Journal of Experimental Biology
KAUST Grant Number:
SA-C0040; UK-C0016
Issue Date:
1-Aug-2012
DOI:
10.1242/jeb.070235
Type:
Article
ISSN:
0022-0949; 1477-9145
Sponsors:
This study was supported by an award from King Abdullah University of Science and Technology [SA-C0040/UK-C0016] and grants from the Research Grants Council of the Hong Kong Special Administrative Region [N_HKUST602/09 and AoE/P-04/04-II] to P.Y.Q.
Additional Links:
http://jeb.biologists.org/cgi/doi/10.1242/jeb.070235
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorZhang, Y.en
dc.contributor.authorHe, L.-S.en
dc.contributor.authorZhang, G.en
dc.contributor.authorXu, Y.en
dc.contributor.authorLee, O.-O.en
dc.contributor.authorMatsumura, K.en
dc.contributor.authorQian, P.-Y.en
dc.date.accessioned2015-05-26T08:08:04Zen
dc.date.available2015-05-26T08:08:04Zen
dc.date.issued2012-08-01en
dc.identifier.citationThe regulatory role of the NO/cGMP signal transduction cascade during larval attachment and metamorphosis of the barnacle Balanus (=Amphibalanus) amphitrite 2012, 215 (21):3813 Journal of Experimental Biologyen
dc.identifier.issn0022-0949en
dc.identifier.issn1477-9145en
dc.identifier.doi10.1242/jeb.070235en
dc.identifier.urihttp://hdl.handle.net/10754/555802en
dc.description.abstractThe barnacle Balanus amphitrite is among the most dominant fouling species on intertidal rocky shores in tropical and subtropical areas and is thus a target organism in antifouling research. After being released from adults, the swimming nauplius undertakes six molting cycles and then transforms into a cyprid. Using paired antennules, a competent cyprid actively explores and selects a suitable substratum for attachment and metamorphosis (collectively known as settlement). This selection process involves the reception of exogenous signals and subsequent endogenous signal transduction. To investigate the involvement of nitric oxide (NO) and cyclic GMP (cGMP) during larval settlement of B. amphitrite, we examined the effects of an NO donor and an NO scavenger, two nitric oxide synthase (NOS) inhibitors and a soluble guanylyl cyclase (sGC) inhibitor on settling cyprids. We found that the NO donor sodium nitroprusside (SNP) inhibited larval settlement in a dose-dependent manner. In contrast, both the NO scavenger carboxy-PTIO and the NOS inhibitors aminoguanidine hemisulfate (AGH) and S-methylisothiourea sulfate (SMIS) significantly accelerated larval settlement. Suppression of the downstream guanylyl cyclase (GC) activity using a GC-selective inhibitor ODQ could also significantly accelerate larval settlement. Interestingly, the settlement inhibition effects of SNP could be attenuated by ODQ at all concentrations tested. In the developmental expression profiling of NOS and sGC, the lowest expression of both genes was detected in the cyprid stage, a crucial stage for the larval decision to attach and metamorphose. In summary, we concluded that NO regulates larval settlement via mediating downstream cGMP signaling.en
dc.description.sponsorshipThis study was supported by an award from King Abdullah University of Science and Technology [SA-C0040/UK-C0016] and grants from the Research Grants Council of the Hong Kong Special Administrative Region [N_HKUST602/09 and AoE/P-04/04-II] to P.Y.Q.en
dc.publisherThe Company of Biologistsen
dc.relation.urlhttp://jeb.biologists.org/cgi/doi/10.1242/jeb.070235en
dc.rightsArchived with thanks to Journal of Experimental Biologyen
dc.subjectbarnacleen
dc.subjectBalanus amphitriteen
dc.subjectlarval attachmenten
dc.subjectmetamorphosisen
dc.subjectlarval settlementen
dc.subjectsignal transductionen
dc.subjectnitric oxideen
dc.subjectguanylyl cyclaseen
dc.subjectcyclic GMPen
dc.titleThe regulatory role of the NO/cGMP signal transduction cascade during larval attachment and metamorphosis of the barnacle Balanus (=Amphibalanus) amphitriteen
dc.typeArticleen
dc.identifier.journalJournal of Experimental Biologyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDivision of Life Science, The Hong Kong University of Science and Technology, Hong Kong SAR, Chinaen
kaust.authorZhang, Y.en
kaust.authorHe, L.-S.en
kaust.authorZhang, G.en
kaust.authorXu, Y.en
kaust.authorLee, O.-O.en
kaust.authorMatsumura, K.en
kaust.authorQian, P.-Y.en
kaust.grant.numberSA-C0040en
kaust.grant.numberUK-C0016en
kaust.grant.programKAUST Global Collaborative Research Programen
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