Plant regeneration of non-toxic Jatropha curcas—impacts of plant growth regulators, source and type of explants

Handle URI:
http://hdl.handle.net/10754/555792
Title:
Plant regeneration of non-toxic Jatropha curcas—impacts of plant growth regulators, source and type of explants
Authors:
Kumar, Nitish; Vijay Anand, K. G.; Reddy, Muppala P.
Abstract:
Jatropha curcas is an oil bearing species with multiple uses and considerable economic potential as a biofuel plant, however, oil and deoiled cake are toxic. A non-toxic variety of J. curcas is reported from Mexico. The present investigation explores the effects of different plant growth regulators (PGRs) viz. 6-benzyl aminopurine (BAP) or thidiazuron (TDZ) individually and in combination with indole-3-butyric acid (IBA), on regeneration from in vitro and field-grown mature leaf explants, in vitro and glasshouse-grown seedlings cotyledonary leaf explants of non-toxic J. curcas. In all the tested parameters maximum regeneration efficiency (81.07%) and the number of shoot buds per explants (20.17) was observed on 9.08 μM TDZ containing Murashige and Skoog’s (MS) medium from in vitro cotyledonary leaf explants. The regenerated shoot buds were transferred to MS medium containing 10 μM kinetin (Kn), 4.5 μM BAP and 5.5 μM α-naphthaleneacetic acid (NAA) for shoot proliferation. The proliferated shoots could be elongated on MS medium supplemented with 2.25 μM BAP and 8.5 μM IAA. Rooting was achieved when the basal cut end of elongated shoots were dipped in half strength MS liquid medium containing different concentrations and combinations of IBA, IAA and NAA for four days followed by transfer to growth regulators free half strength MS medium supplemented 0.25 mg/l activated charcoal. The rooted plants could be established in soil with more than 90% survival rate.
KAUST Department:
Plant Stress Genomics Research Lab
Citation:
Plant regeneration of non-toxic Jatropha curcas—impacts of plant growth regulators, source and type of explants 2011, 20 (1):125 Journal of Plant Biochemistry and Biotechnology
Journal:
Journal of Plant Biochemistry and Biotechnology
Issue Date:
28-Jan-2011
DOI:
10.1007/s13562-011-0037-6
Type:
Article
ISSN:
0971-7811; 0974-1275
Additional Links:
http://link.springer.com/10.1007/s13562-011-0037-6
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorKumar, Nitishen
dc.contributor.authorVijay Anand, K. G.en
dc.contributor.authorReddy, Muppala P.en
dc.date.accessioned2015-05-26T08:00:28Zen
dc.date.available2015-05-26T08:00:28Zen
dc.date.issued2011-01-28en
dc.identifier.citationPlant regeneration of non-toxic Jatropha curcas—impacts of plant growth regulators, source and type of explants 2011, 20 (1):125 Journal of Plant Biochemistry and Biotechnologyen
dc.identifier.issn0971-7811en
dc.identifier.issn0974-1275en
dc.identifier.doi10.1007/s13562-011-0037-6en
dc.identifier.urihttp://hdl.handle.net/10754/555792en
dc.description.abstractJatropha curcas is an oil bearing species with multiple uses and considerable economic potential as a biofuel plant, however, oil and deoiled cake are toxic. A non-toxic variety of J. curcas is reported from Mexico. The present investigation explores the effects of different plant growth regulators (PGRs) viz. 6-benzyl aminopurine (BAP) or thidiazuron (TDZ) individually and in combination with indole-3-butyric acid (IBA), on regeneration from in vitro and field-grown mature leaf explants, in vitro and glasshouse-grown seedlings cotyledonary leaf explants of non-toxic J. curcas. In all the tested parameters maximum regeneration efficiency (81.07%) and the number of shoot buds per explants (20.17) was observed on 9.08 μM TDZ containing Murashige and Skoog’s (MS) medium from in vitro cotyledonary leaf explants. The regenerated shoot buds were transferred to MS medium containing 10 μM kinetin (Kn), 4.5 μM BAP and 5.5 μM α-naphthaleneacetic acid (NAA) for shoot proliferation. The proliferated shoots could be elongated on MS medium supplemented with 2.25 μM BAP and 8.5 μM IAA. Rooting was achieved when the basal cut end of elongated shoots were dipped in half strength MS liquid medium containing different concentrations and combinations of IBA, IAA and NAA for four days followed by transfer to growth regulators free half strength MS medium supplemented 0.25 mg/l activated charcoal. The rooted plants could be established in soil with more than 90% survival rate.en
dc.relation.urlhttp://link.springer.com/10.1007/s13562-011-0037-6en
dc.rightsArchived with thanks to Journal of Plant Biochemistry and Biotechnologyen
dc.titlePlant regeneration of non-toxic Jatropha curcas—impacts of plant growth regulators, source and type of explantsen
dc.typeArticleen
dc.contributor.departmentPlant Stress Genomics Research Laben
dc.identifier.journalJournal of Plant Biochemistry and Biotechnologyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionCentre for Biotechnology, School of Earth, Biological and Environmental Science, Central University of Bihar, Patna, 800014, Bihar, Indiaen
dc.contributor.institutionDiscipline of Wasteland Research, Central Salt & Marine Chemicals Research Institute (Council of Scientific and Industrial Research), Bhavnagar, Gujarat, 364002, Indiaen
kaust.authorReddy, Muppala P.en
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