Mitochondrial ribosomal protein S18-2 evokes chromosomal instability and transforms primary rat skin fibroblasts

Handle URI:
http://hdl.handle.net/10754/555631
Title:
Mitochondrial ribosomal protein S18-2 evokes chromosomal instability and transforms primary rat skin fibroblasts
Authors:
Kashuba, Elena; Carbone, Ennio; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 ) ; Tirinato, Luca ( 0000-0001-9826-2129 ) ; Petruchek, Maria; Drummond, Catherine; Kovalevska, Larysa; Gurrapu, Sreeharsha; Mushtaq, Muhammad; Darekar, Suhas D.
Abstract:
We have shown earlier that overexpression of the human mitochondrial ribosomal protein MRPS18-2 (S18-2) led to immortalization of primary rat embryonic fibroblasts. The derived cells expressed the embryonic stem cell markers, and cellular pathways that control cell proliferation, oxidative phosphorylation, cellular respiration, and other redox reactions were activated in the immortalized cells. Here we report that, upon overexpression of S18-2 protein, primary rat skin fibroblasts underwent cell transformation. Cells passed more than 300 population doublings, and two out of three tested clones gave rise to tumors in experimental animals. Transformed cells showed anchorage-independent growth and loss of contact inhibition; they expressed epithelial markers, such as E-cadherin and β-catenin. Transformed cells showed increased telomerase activity, disturbance of the cell cycle, and chromosomal instability. Taken together, our data suggest that S18-2 is a newly identified oncoprotein that may be involved in cancerogenesis.
KAUST Department:
Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Darekar, Suhas D., Muhammad Mushtaq, Larysa Sreeharsha Gurrapu, Catherine Drummond Kovalevska, Maria Petruchek, Luca Tirinato, Enzo Di, Ennio Carbone Fabrizio, and Elena Kashuba. "Mitochondrial ribosomal protein S18-2 evokes chromosomal instability and transforms primary rat skin fibroblasts."
Publisher:
Impact Journals
Journal:
Oncotarget
Issue Date:
12-May-2015
Type:
Article
ISSN:
1949-2553
Additional Links:
http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=4123
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorKashuba, Elenaen
dc.contributor.authorCarbone, Ennioen
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.contributor.authorTirinato, Lucaen
dc.contributor.authorPetruchek, Mariaen
dc.contributor.authorDrummond, Catherineen
dc.contributor.authorKovalevska, Larysaen
dc.contributor.authorGurrapu, Sreeharshaen
dc.contributor.authorMushtaq, Muhammaden
dc.contributor.authorDarekar, Suhas D.en
dc.date.accessioned2015-05-25T07:56:48Zen
dc.date.available2015-05-25T07:56:48Zen
dc.date.issued2015-05-12en
dc.identifier.citationDarekar, Suhas D., Muhammad Mushtaq, Larysa Sreeharsha Gurrapu, Catherine Drummond Kovalevska, Maria Petruchek, Luca Tirinato, Enzo Di, Ennio Carbone Fabrizio, and Elena Kashuba. "Mitochondrial ribosomal protein S18-2 evokes chromosomal instability and transforms primary rat skin fibroblasts."en
dc.identifier.issn1949-2553en
dc.identifier.urihttp://hdl.handle.net/10754/555631en
dc.description.abstractWe have shown earlier that overexpression of the human mitochondrial ribosomal protein MRPS18-2 (S18-2) led to immortalization of primary rat embryonic fibroblasts. The derived cells expressed the embryonic stem cell markers, and cellular pathways that control cell proliferation, oxidative phosphorylation, cellular respiration, and other redox reactions were activated in the immortalized cells. Here we report that, upon overexpression of S18-2 protein, primary rat skin fibroblasts underwent cell transformation. Cells passed more than 300 population doublings, and two out of three tested clones gave rise to tumors in experimental animals. Transformed cells showed anchorage-independent growth and loss of contact inhibition; they expressed epithelial markers, such as E-cadherin and β-catenin. Transformed cells showed increased telomerase activity, disturbance of the cell cycle, and chromosomal instability. Taken together, our data suggest that S18-2 is a newly identified oncoprotein that may be involved in cancerogenesis.en
dc.language.isoenen
dc.publisherImpact Journalsen
dc.relation.urlhttp://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=4123en
dc.rightsAll site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License. http://creativecommons.org/licenses/by/3.0/en
dc.subjectMRPS18-2en
dc.subjectcell transformationen
dc.subjectmitochondrial ribosomal proteinen
dc.subjectrat skin fibroblastsen
dc.subjectchromosomal instabilityen
dc.titleMitochondrial ribosomal protein S18-2 evokes chromosomal instability and transforms primary rat skin fibroblastsen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalOncotargeten
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Swedenen
dc.contributor.institutionR.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NASU, Ukraineen
dc.contributor.institutionUniversity “Magna Græcia” of Catanzaro, Viale Europa, Località Germaneto, Catanzaro, Italyen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
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