Dissolved Organic Carbon Influences Microbial Community Composition and Diversity in Managed Aquifer Recharge Systems

Handle URI:
http://hdl.handle.net/10754/554121
Title:
Dissolved Organic Carbon Influences Microbial Community Composition and Diversity in Managed Aquifer Recharge Systems
Authors:
Li, D.; Sharp, J. O.; Saikaly, Pascal ( 0000-0001-7678-3986 ) ; Ali, Shahjahan; Alidina, M.; Alarawi, M. S.; Keller, S.; Hoppe-Jones, C.; Drewes, J. E.
Abstract:
This study explores microbial community structure in managed aquifer recharge (MAR) systems across both laboratory and field scales. Two field sites, the Taif River (Taif, Saudi Arabia) and South Platte River (Colorado), were selected as geographically distinct MAR systems. Samples derived from unsaturated riverbed, saturated-shallow-infiltration (depth, 1 to 2 cm), and intermediate-infiltration (depth, 10 to 50 cm) zones were collected. Complementary laboratory-scale sediment columns representing low (0.6 mg/liter) and moderate (5 mg/liter) dissolved organic carbon (DOC) concentrations were used to further query the influence of DOC and depth on microbial assemblages. Microbial density was positively correlated with the DOC concentration, while diversity was negatively correlated at both the laboratory and field scales. Microbial communities derived from analogous sampling zones in each river were not phylogenetically significantly different on phylum, class, genus, and species levels, as determined by 16S rRNA gene pyrosequencing, suggesting that geography and season exerted less sway than aqueous geochemical properties. When field-scale communities derived from the Taif and South Platte River sediments were grouped together, principal coordinate analysis revealed distinct clusters with regard to the three sample zones (unsaturated, shallow, and intermediate saturated) and, further, with respect to DOC concentration. An analogous trend as a function of depth and corresponding DOC loss was observed in column studies. Canonical correspondence analysis suggests that microbial classes Betaproteobacteria and Gammaproteobacteria are positively correlated with DOC concentration. Our combined analyses at both the laboratory and field scales suggest that DOC may exert a strong influence on microbial community composition and diversity in MAR saturated zones.
KAUST Department:
Water Desalination and Reuse Research Center (WDRC); Biosciences Core Lab
Citation:
Dissolved Organic Carbon Influences Microbial Community Composition and Diversity in Managed Aquifer Recharge Systems 2012, 78 (19):6819 Applied and Environmental Microbiology
Journal:
Applied and Environmental Microbiology
Issue Date:
13-Jul-2012
DOI:
10.1128/AEM.01223-12
PubMed ID:
22798375
PubMed Central ID:
PMC3457517
Type:
Article
ISSN:
0099-2240
Additional Links:
http://aem.asm.org/cgi/doi/10.1128/AEM.01223-12
Appears in Collections:
Articles; Biosciences Core Lab; Biosciences Core Lab; Water Desalination and Reuse Research Center (WDRC); Water Desalination and Reuse Research Center (WDRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorLi, D.en
dc.contributor.authorSharp, J. O.en
dc.contributor.authorSaikaly, Pascalen
dc.contributor.authorAli, Shahjahanen
dc.contributor.authorAlidina, M.en
dc.contributor.authorAlarawi, M. S.en
dc.contributor.authorKeller, S.en
dc.contributor.authorHoppe-Jones, C.en
dc.contributor.authorDrewes, J. E.en
dc.date.accessioned2015-05-18T21:49:38Zen
dc.date.available2015-05-18T21:49:38Zen
dc.date.issued2012-07-13en
dc.identifier.citationDissolved Organic Carbon Influences Microbial Community Composition and Diversity in Managed Aquifer Recharge Systems 2012, 78 (19):6819 Applied and Environmental Microbiologyen
dc.identifier.issn0099-2240en
dc.identifier.pmid22798375en
dc.identifier.doi10.1128/AEM.01223-12en
dc.identifier.urihttp://hdl.handle.net/10754/554121en
dc.description.abstractThis study explores microbial community structure in managed aquifer recharge (MAR) systems across both laboratory and field scales. Two field sites, the Taif River (Taif, Saudi Arabia) and South Platte River (Colorado), were selected as geographically distinct MAR systems. Samples derived from unsaturated riverbed, saturated-shallow-infiltration (depth, 1 to 2 cm), and intermediate-infiltration (depth, 10 to 50 cm) zones were collected. Complementary laboratory-scale sediment columns representing low (0.6 mg/liter) and moderate (5 mg/liter) dissolved organic carbon (DOC) concentrations were used to further query the influence of DOC and depth on microbial assemblages. Microbial density was positively correlated with the DOC concentration, while diversity was negatively correlated at both the laboratory and field scales. Microbial communities derived from analogous sampling zones in each river were not phylogenetically significantly different on phylum, class, genus, and species levels, as determined by 16S rRNA gene pyrosequencing, suggesting that geography and season exerted less sway than aqueous geochemical properties. When field-scale communities derived from the Taif and South Platte River sediments were grouped together, principal coordinate analysis revealed distinct clusters with regard to the three sample zones (unsaturated, shallow, and intermediate saturated) and, further, with respect to DOC concentration. An analogous trend as a function of depth and corresponding DOC loss was observed in column studies. Canonical correspondence analysis suggests that microbial classes Betaproteobacteria and Gammaproteobacteria are positively correlated with DOC concentration. Our combined analyses at both the laboratory and field scales suggest that DOC may exert a strong influence on microbial community composition and diversity in MAR saturated zones.en
dc.relation.urlhttp://aem.asm.org/cgi/doi/10.1128/AEM.01223-12en
dc.rightsArchived with thanks to Applied and Environmental Microbiologyen
dc.titleDissolved Organic Carbon Influences Microbial Community Composition and Diversity in Managed Aquifer Recharge Systemsen
dc.typeArticleen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.contributor.departmentBiosciences Core Laben
dc.identifier.journalApplied and Environmental Microbiologyen
dc.identifier.pmcidPMC3457517en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionNSF Engineering Research Center ReNUWIt, Civil and Environmental Engineering, Colorado School of Mines, Golden, Colorado, USAen
kaust.authorLi, Dongen
kaust.authorSaikaly, Pascalen
kaust.authorAli, Shahjahanen
kaust.authorAlidina, Mazahiralien
kaust.authorAlarawi, Mohammeden
kaust.authorDrewes, Jorgen
kaust.authorKeller, Stephanieen

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