Spatial and Species Variations in Bacterial Communities Associated with Corals from the Red Sea as Revealed by Pyrosequencing

Handle URI:
http://hdl.handle.net/10754/554103
Title:
Spatial and Species Variations in Bacterial Communities Associated with Corals from the Red Sea as Revealed by Pyrosequencing
Authors:
Lee, O. O.; Yang, J.; Bougouffa, S.; Wang, Y.; Batang, Zenon B. ( 0000-0003-4593-0589 ) ; Tian, R.; Al-Suwailem, A.; Qian, P.-Y.
Abstract:
Microbial associations with corals are common and are most likely symbiotic, although their diversity and relationships with environmental factors and host species remain unclear. In this study, we adopted a 16S rRNA gene tag-pyrosequencing technique to investigate the bacterial communities associated with three stony Scleractinea and two soft Octocorallia corals from three locations in the Red Sea. Our results revealed highly diverse bacterial communities in the Red Sea corals, with more than 600 ribotypes detected and up to 1,000 species estimated from a single coral species. Altogether, 21 bacterial phyla were recovered from the corals, of which Gammaproteobacteria was the most dominant group, and Chloroflexi, Chlamydiae, and the candidate phylum WS3 were reported in corals for the first time. The associated bacterial communities varied greatly with location, where environmental conditions differed significantly. Corals from disturbed areas appeared to share more similar bacterial communities, but larger variations in community structures were observed between different coral species from pristine waters. Ordination methods identified salinity and depth as the most influential parameters affecting the abundance of Vibrio, Pseudoalteromonas, Serratia, Stenotrophomonas, Pseudomonas, and Achromobacter in the corals. On the other hand, bacteria such as Chloracidobacterium and Endozoicomonas were more sensitive to the coral species, suggesting that the host species type may be influential in the associated bacterial community, as well. The combined influences of the coral host and environmental factors on the associated microbial communities are discussed. This study represents the first comparative study using tag-pyrosequencing technology to investigate the bacterial communities in Red Sea corals.
KAUST Department:
Coastal and Marine Resources Core Lab
Citation:
Spatial and Species Variations in Bacterial Communities Associated with Corals from the Red Sea as Revealed by Pyrosequencing 2012, 78 (20):7173 Applied and Environmental Microbiology
Publisher:
American Society for Microbiology
Journal:
Applied and Environmental Microbiology
Issue Date:
3-Aug-2012
DOI:
10.1128/AEM.01111-12
PubMed ID:
22865078
PubMed Central ID:
PMC3457102
Type:
Article
ISSN:
0099-2240
Additional Links:
http://aem.asm.org/cgi/doi/10.1128/AEM.01111-12
Appears in Collections:
Articles; Coastal and Marine Resources Core Lab; Coastal and Marine Resources Core Lab

Full metadata record

DC FieldValue Language
dc.contributor.authorLee, O. O.en
dc.contributor.authorYang, J.en
dc.contributor.authorBougouffa, S.en
dc.contributor.authorWang, Y.en
dc.contributor.authorBatang, Zenon B.en
dc.contributor.authorTian, R.en
dc.contributor.authorAl-Suwailem, A.en
dc.contributor.authorQian, P.-Y.en
dc.date.accessioned2015-05-18T21:51:02Zen
dc.date.available2015-05-18T21:51:02Zen
dc.date.issued2012-08-03en
dc.identifier.citationSpatial and Species Variations in Bacterial Communities Associated with Corals from the Red Sea as Revealed by Pyrosequencing 2012, 78 (20):7173 Applied and Environmental Microbiologyen
dc.identifier.issn0099-2240en
dc.identifier.pmid22865078en
dc.identifier.doi10.1128/AEM.01111-12en
dc.identifier.urihttp://hdl.handle.net/10754/554103en
dc.description.abstractMicrobial associations with corals are common and are most likely symbiotic, although their diversity and relationships with environmental factors and host species remain unclear. In this study, we adopted a 16S rRNA gene tag-pyrosequencing technique to investigate the bacterial communities associated with three stony Scleractinea and two soft Octocorallia corals from three locations in the Red Sea. Our results revealed highly diverse bacterial communities in the Red Sea corals, with more than 600 ribotypes detected and up to 1,000 species estimated from a single coral species. Altogether, 21 bacterial phyla were recovered from the corals, of which Gammaproteobacteria was the most dominant group, and Chloroflexi, Chlamydiae, and the candidate phylum WS3 were reported in corals for the first time. The associated bacterial communities varied greatly with location, where environmental conditions differed significantly. Corals from disturbed areas appeared to share more similar bacterial communities, but larger variations in community structures were observed between different coral species from pristine waters. Ordination methods identified salinity and depth as the most influential parameters affecting the abundance of Vibrio, Pseudoalteromonas, Serratia, Stenotrophomonas, Pseudomonas, and Achromobacter in the corals. On the other hand, bacteria such as Chloracidobacterium and Endozoicomonas were more sensitive to the coral species, suggesting that the host species type may be influential in the associated bacterial community, as well. The combined influences of the coral host and environmental factors on the associated microbial communities are discussed. This study represents the first comparative study using tag-pyrosequencing technology to investigate the bacterial communities in Red Sea corals.en
dc.publisherAmerican Society for Microbiologyen
dc.relation.urlhttp://aem.asm.org/cgi/doi/10.1128/AEM.01111-12en
dc.rightsArchived with thanks to Applied and Environmental Microbiologyen
dc.titleSpatial and Species Variations in Bacterial Communities Associated with Corals from the Red Sea as Revealed by Pyrosequencingen
dc.typeArticleen
dc.contributor.departmentCoastal and Marine Resources Core Laben
dc.identifier.journalApplied and Environmental Microbiologyen
dc.identifier.pmcidPMC3457102en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDivision of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kongen
kaust.authorWang, Yongen
kaust.authorAl-Suwailem, Abdulaziz M.en
kaust.authorBatang, Zenon B.en

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