Monitoring the Perturbation of Soil and Groundwater Microbial Communities Due to Pig Production Activities

Handle URI:
http://hdl.handle.net/10754/554096
Title:
Monitoring the Perturbation of Soil and Groundwater Microbial Communities Due to Pig Production Activities
Authors:
Hong, Pei-Ying ( 0000-0002-4474-6600 ) ; Yannarell, A. C.; Dai, Q.; Ekizoglu, M.; Mackie, R. I.
Abstract:
This study aimed to determine if biotic contaminants originating from pig production farms are disseminated into soil and groundwater microbial communities. A spatial and temporal sampling of soil and groundwater in proximity to pig production farms was conducted, and quantitative PCR (Q-PCR) was utilized to determine the abundances of tetracycline resistance genes (i.e., tetQ and tetZ) and integrase genes (i.e., intI1 and intI2). We observed that the abundances of tetZ, tetQ, intI1, and intI2 in the soils increased at least 6-fold after manure application, and their abundances remained elevated above the background for up to 16 months. Q-PCR further determined total abundances of up to 5.88 × 109 copies/ng DNA for tetZ, tetQ, intI1, and intI2 in some of the groundwater wells that were situated next to the manure lagoon and in the facility well used to supply water for one of the farms. We further utilized 16S rRNA-based pyrosequencing to assess the microbial communities, and our comparative analyses suggest that most of the soil samples collected before and after manure application did not change significantly, sharing a high Bray-Curtis similarity of 78.5%. In contrast, an increase in Bacteroidetes and sulfur-oxidizing bacterial populations was observed in the groundwaters collected from lagoon-associated groundwater wells. Genera associated with opportunistic human and animal pathogens, such as Acinetobacter, Arcobacter, Yersinia, and Coxiella, were detected in some of the manure-treated soils and affected groundwater wells. Feces-associated bacteria such as Streptococcus, Erysipelothrix, and Bacteroides were detected in the manure, soil, and groundwater ecosystems, suggesting a perturbation of the soil and groundwater environments by invader species from pig production activities.
KAUST Department:
Environmental Science and Engineering Program; Water Desalination and Reuse Research Center (WDRC)
Citation:
Monitoring the Perturbation of Soil and Groundwater Microbial Communities Due to Pig Production Activities 2013, 79 (8):2620 Applied and Environmental Microbiology
Publisher:
American Society for Microbiology
Journal:
Applied and Environmental Microbiology
Issue Date:
8-Feb-2013
DOI:
10.1128/AEM.03760-12
PubMed ID:
23396341
PubMed Central ID:
PMC3623201
Type:
Article
ISSN:
0099-2240
Additional Links:
http://aem.asm.org/cgi/doi/10.1128/AEM.03760-12
Appears in Collections:
Articles; Environmental Science and Engineering Program; Water Desalination and Reuse Research Center (WDRC); Water Desalination and Reuse Research Center (WDRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorHong, Pei-Yingen
dc.contributor.authorYannarell, A. C.en
dc.contributor.authorDai, Q.en
dc.contributor.authorEkizoglu, M.en
dc.contributor.authorMackie, R. I.en
dc.date.accessioned2015-05-18T21:24:41Zen
dc.date.available2015-05-18T21:24:41Zen
dc.date.issued2013-02-08en
dc.identifier.citationMonitoring the Perturbation of Soil and Groundwater Microbial Communities Due to Pig Production Activities 2013, 79 (8):2620 Applied and Environmental Microbiologyen
dc.identifier.issn0099-2240en
dc.identifier.pmid23396341en
dc.identifier.doi10.1128/AEM.03760-12en
dc.identifier.urihttp://hdl.handle.net/10754/554096en
dc.description.abstractThis study aimed to determine if biotic contaminants originating from pig production farms are disseminated into soil and groundwater microbial communities. A spatial and temporal sampling of soil and groundwater in proximity to pig production farms was conducted, and quantitative PCR (Q-PCR) was utilized to determine the abundances of tetracycline resistance genes (i.e., tetQ and tetZ) and integrase genes (i.e., intI1 and intI2). We observed that the abundances of tetZ, tetQ, intI1, and intI2 in the soils increased at least 6-fold after manure application, and their abundances remained elevated above the background for up to 16 months. Q-PCR further determined total abundances of up to 5.88 × 109 copies/ng DNA for tetZ, tetQ, intI1, and intI2 in some of the groundwater wells that were situated next to the manure lagoon and in the facility well used to supply water for one of the farms. We further utilized 16S rRNA-based pyrosequencing to assess the microbial communities, and our comparative analyses suggest that most of the soil samples collected before and after manure application did not change significantly, sharing a high Bray-Curtis similarity of 78.5%. In contrast, an increase in Bacteroidetes and sulfur-oxidizing bacterial populations was observed in the groundwaters collected from lagoon-associated groundwater wells. Genera associated with opportunistic human and animal pathogens, such as Acinetobacter, Arcobacter, Yersinia, and Coxiella, were detected in some of the manure-treated soils and affected groundwater wells. Feces-associated bacteria such as Streptococcus, Erysipelothrix, and Bacteroides were detected in the manure, soil, and groundwater ecosystems, suggesting a perturbation of the soil and groundwater environments by invader species from pig production activities.en
dc.publisherAmerican Society for Microbiologyen
dc.relation.urlhttp://aem.asm.org/cgi/doi/10.1128/AEM.03760-12en
dc.rightsArchived with thanks to Applied and Environmental Microbiologyen
dc.titleMonitoring the Perturbation of Soil and Groundwater Microbial Communities Due to Pig Production Activitiesen
dc.typeArticleen
dc.contributor.departmentEnvironmental Science and Engineering Programen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalApplied and Environmental Microbiologyen
dc.identifier.pmcidPMC3623201en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Animal Sciencesen
dc.contributor.institutionInstitute of Genomic Biologyen
dc.contributor.institutionDepartment of Natural Resources and Environmental Sciencesen
dc.contributor.institutionUniversity of Illinois at Urbana-Champaign, Urbana, Illinois, USAen
dc.contributor.institutionCollege of Engineering, Nanjing Agricultural University, Nanjing, Jiangsu Province, Chinaen
dc.contributor.institutionDepartment of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Hacettepe, Ankara, Turkeyen
kaust.authorHong, Pei-Yingen
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.