Use of the Plant Defense Protein Osmotin To Identify Fusarium oxysporum Genes That Control Cell Wall Properties

Handle URI:
http://hdl.handle.net/10754/553078
Title:
Use of the Plant Defense Protein Osmotin To Identify Fusarium oxysporum Genes That Control Cell Wall Properties
Authors:
Lee, H.; Damsz, B.; Woloshuk, C. P.; Bressan, R. A.; Narasimhan, Meena L.
Abstract:
Fusarium oxysporum is the causative agent of fungal wilt disease in a variety of crops. The capacity of a fungal pathogen such as F. oxysporum f. sp. nicotianae to establish infection on its tobacco (Nicotiana tabacum) host depends in part on its capacity to evade the toxicity of tobacco defense proteins, such as osmotin. Fusarium genes that control resistance to osmotin would therefore reflect coevolutionary pressures and include genes that control mutual recognition, avoidance, and detoxification. We identified FOR (Fusarium Osmotin Resistance) genes on the basis of their ability to confer osmotin resistance to an osmotin-sensitive strain of Saccharomyces cerevisiae. FOR1 encodes a putative cell wall glycoprotein. FOR2 encodes the structural gene for glutamine:fructose-6-phosphate amidotransferase, the first and rate-limiting step in the biosynthesis of hexosamine and cell wall chitin. FOR3 encodes a homolog of SSD1, which controls cell wall composition, longevity, and virulence in S. cerevisiae. A for3 null mutation increased osmotin sensitivity of conidia and hyphae of F. oxysporum f. sp. nicotianae and also reduced cell wall β-1,3-glucan content. Together our findings show that conserved fungal genes that determine cell wall properties play a crucial role in regulating fungal susceptibility to the plant defense protein osmotin.
KAUST Department:
Plant Stress Genomics Research Lab
Citation:
Use of the Plant Defense Protein Osmotin To Identify Fusarium oxysporum Genes That Control Cell Wall Properties 2010, 9 (4):558 Eukaryotic Cell
Publisher:
American Society for Microbiology
Journal:
Eukaryotic Cell
Issue Date:
26-Feb-2010
DOI:
10.1128/EC.00316-09
PubMed ID:
20190074
PubMed Central ID:
PMC2863404
Type:
Article
ISSN:
1535-9778; 1535-9786
Additional Links:
http://ec.asm.org/cgi/doi/10.1128/EC.00316-09
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorLee, H.en
dc.contributor.authorDamsz, B.en
dc.contributor.authorWoloshuk, C. P.en
dc.contributor.authorBressan, R. A.en
dc.contributor.authorNarasimhan, Meena L.en
dc.date.accessioned2015-05-18T21:23:33Zen
dc.date.available2015-05-18T21:23:33Zen
dc.date.issued2010-02-26en
dc.identifier.citationUse of the Plant Defense Protein Osmotin To Identify Fusarium oxysporum Genes That Control Cell Wall Properties 2010, 9 (4):558 Eukaryotic Cellen
dc.identifier.issn1535-9778en
dc.identifier.issn1535-9786en
dc.identifier.pmid20190074en
dc.identifier.doi10.1128/EC.00316-09en
dc.identifier.urihttp://hdl.handle.net/10754/553078en
dc.description.abstractFusarium oxysporum is the causative agent of fungal wilt disease in a variety of crops. The capacity of a fungal pathogen such as F. oxysporum f. sp. nicotianae to establish infection on its tobacco (Nicotiana tabacum) host depends in part on its capacity to evade the toxicity of tobacco defense proteins, such as osmotin. Fusarium genes that control resistance to osmotin would therefore reflect coevolutionary pressures and include genes that control mutual recognition, avoidance, and detoxification. We identified FOR (Fusarium Osmotin Resistance) genes on the basis of their ability to confer osmotin resistance to an osmotin-sensitive strain of Saccharomyces cerevisiae. FOR1 encodes a putative cell wall glycoprotein. FOR2 encodes the structural gene for glutamine:fructose-6-phosphate amidotransferase, the first and rate-limiting step in the biosynthesis of hexosamine and cell wall chitin. FOR3 encodes a homolog of SSD1, which controls cell wall composition, longevity, and virulence in S. cerevisiae. A for3 null mutation increased osmotin sensitivity of conidia and hyphae of F. oxysporum f. sp. nicotianae and also reduced cell wall β-1,3-glucan content. Together our findings show that conserved fungal genes that determine cell wall properties play a crucial role in regulating fungal susceptibility to the plant defense protein osmotin.en
dc.publisherAmerican Society for Microbiologyen
dc.relation.urlhttp://ec.asm.org/cgi/doi/10.1128/EC.00316-09en
dc.rightsArchived with thanks to Eukaryotic Cellen
dc.titleUse of the Plant Defense Protein Osmotin To Identify Fusarium oxysporum Genes That Control Cell Wall Propertiesen
dc.typeArticleen
dc.contributor.departmentPlant Stress Genomics Research Laben
dc.identifier.journalEukaryotic Cellen
dc.identifier.pmcidPMC2863404en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartments of Horticulture and Landscape Architectureen
dc.contributor.institutionBotany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907en
kaust.authorBressan, Ray Anthonyen
kaust.authorNarasimhan, Meena L.en

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