Studies on the structure and function of intestinal microbes of surgeonfishes in the central Red Sea with a focus on the giant bacteria Epulopiscium spp.

Handle URI:
http://hdl.handle.net/10754/552840
Title:
Studies on the structure and function of intestinal microbes of surgeonfishes in the central Red Sea with a focus on the giant bacteria Epulopiscium spp.
Authors:
Miyake, Sou ( 0000-0001-7507-1653 )
Abstract:
The intestinal tract microbiota – microbial community of the gut – is an important field in microbiology not only because of its critical role in the host development, but also increasingly large number of diseases are associated with certain state of the gut microbiota. The community structure and function of the gut microbiota is relatively well studied in humans and related higher vertebrates, but is severely understudied in fish. This is especially true for the coral reef fishes, who constitute the most diverse assemblage of vertebrates spread over a very local scale, and are essential for the resilience of the reefs. In order to bridge this gap in knowledge, this dissertation studied the community structure, interactions and functions of the gut microbial community from the surgeonfishes in the Red Sea – with special focus on the surgeonfish enteric symbiont Epulopiscium spp. Initially, I studied the composition of the gut microbiota of nine surgeonfish and three nonsurgeonfish species from the Red Sea using 454 pyrosequencing. Upon discovering the high abundance of Epulopiscium spp. in herbivorous surgeonfishes, I then proceeded to identify their phylogenetic diversity, distribution, as well as deducing their coevolutionary relationship with the host. Because Epulopiscium spp. undergo substantial changes in the cell size (grow up to ~600μm) and the DNA concentration (from 85 to over 250pg per cell) throughout their diel lifecycle, I also studied the temporal changes in their expression pattern using RNA-seq. Overall, this dissertation shed light on the complex structure, interaction and function of an important family of coral reef fish from the Red Sea through range of molecular techniques.
Advisors:
Stingl, Ulrich ( 0000-0002-0684-2597 )
Committee Member:
Berumen, Michael L. ( 0000-0003-2463-2742 ) ; Pain, Arnab ( 0000-0002-1755-2819 ) ; Klebensberger, Janosch
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Program:
Marine Science
Issue Date:
May-2015
Type:
Dissertation
Appears in Collections:
Marine Science Program; Dissertations; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.advisorStingl, Ulrichen
dc.contributor.authorMiyake, Souen
dc.date.accessioned2015-05-14T08:50:06Zen
dc.date.available2015-05-14T08:50:06Zen
dc.date.issued2015-05en
dc.identifier.urihttp://hdl.handle.net/10754/552840en
dc.description.abstractThe intestinal tract microbiota – microbial community of the gut – is an important field in microbiology not only because of its critical role in the host development, but also increasingly large number of diseases are associated with certain state of the gut microbiota. The community structure and function of the gut microbiota is relatively well studied in humans and related higher vertebrates, but is severely understudied in fish. This is especially true for the coral reef fishes, who constitute the most diverse assemblage of vertebrates spread over a very local scale, and are essential for the resilience of the reefs. In order to bridge this gap in knowledge, this dissertation studied the community structure, interactions and functions of the gut microbial community from the surgeonfishes in the Red Sea – with special focus on the surgeonfish enteric symbiont Epulopiscium spp. Initially, I studied the composition of the gut microbiota of nine surgeonfish and three nonsurgeonfish species from the Red Sea using 454 pyrosequencing. Upon discovering the high abundance of Epulopiscium spp. in herbivorous surgeonfishes, I then proceeded to identify their phylogenetic diversity, distribution, as well as deducing their coevolutionary relationship with the host. Because Epulopiscium spp. undergo substantial changes in the cell size (grow up to ~600μm) and the DNA concentration (from 85 to over 250pg per cell) throughout their diel lifecycle, I also studied the temporal changes in their expression pattern using RNA-seq. Overall, this dissertation shed light on the complex structure, interaction and function of an important family of coral reef fish from the Red Sea through range of molecular techniques.en
dc.language.isoenen
dc.subjectGut Microbiotaen
dc.subjectEpulopisciumen
dc.subjectcommunity compositionen
dc.subjectDifferential Expressionen
dc.subjectCoevolutionen
dc.subjectDiel Lifecycleen
dc.titleStudies on the structure and function of intestinal microbes of surgeonfishes in the central Red Sea with a focus on the giant bacteria Epulopiscium spp.en
dc.typeDissertationen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
thesis.degree.grantorKing Abdullah University of Science and Technologyen_GB
dc.contributor.committeememberBerumen, Michael L.en
dc.contributor.committeememberPain, Arnaben
dc.contributor.committeememberKlebensberger, Janoschen
thesis.degree.disciplineMarine Scienceen
thesis.degree.nameDoctor of Philosophyen
dc.person.id101863en
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