Molecular cloning and characterization of Polygalacturonase-Inhibiting Protein and Cinnamoyl-Coa Reductase genes and their association with fruit storage conditions in blueberry (Vaccinium corymbosum)

Handle URI:
http://hdl.handle.net/10754/552461
Title:
Molecular cloning and characterization of Polygalacturonase-Inhibiting Protein and Cinnamoyl-Coa Reductase genes and their association with fruit storage conditions in blueberry (Vaccinium corymbosum)
Authors:
Khraiwesh, Basel; Harb, Jamil; Qudeimat, Enas
Abstract:
Blueberry is a widely grown and easily perishable fruit crop. An efficient post-harvest handling is critical, and for that purpose gene technology methods have been part of ongoing programmes to improve crops with high food values such as blueberry. Here we report the isolation, cloning, characterization and differential expression levels of two cDNAs encoding Polygalacturonase-Inhibitor Protein (PGIP) and Cinnamoyl-Coa Reductase (CCR) from blueberry fruits in relation to various storage conditions. The open reading frame of PGIP and CCR encodes a polypeptide of 329 and 347 amino acids, respectively. To assess changes in the expression of blueberry PGIP and CCR after harvest, a storage trial was initiated. The northern blots hybridization showed a clear differential expression level of PGIP and CCR between freshly harvested and stored fruits as well as between fruits stored under various storage conditions. Although the prospects of exploiting such a strategy for crop improvement are limited, the results provide further insight into the control of the quality over the storage period at the molecular level.
KAUST Department:
Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Molecular cloning and characterization of Polygalacturonase-Inhibiting Protein and Cinnamoyl-Coa Reductase genes and their association with fruit storage conditions in blueberry (Vaccinium corymbosum) 2013, 11 (1):1 Journal of Genetic Engineering and Biotechnology
Journal:
Journal of Genetic Engineering and Biotechnology
Issue Date:
13-May-2013
DOI:
10.1016/j.jgeb.2013.04.003
Type:
Article
ISSN:
1687157X
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S1687157X13000097
Appears in Collections:
Articles; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorKhraiwesh, Baselen
dc.contributor.authorHarb, Jamilen
dc.contributor.authorQudeimat, Enasen
dc.date.accessioned2015-05-07T14:18:03Zen
dc.date.available2015-05-07T14:18:03Zen
dc.date.issued2013-05-13en
dc.identifier.citationMolecular cloning and characterization of Polygalacturonase-Inhibiting Protein and Cinnamoyl-Coa Reductase genes and their association with fruit storage conditions in blueberry (Vaccinium corymbosum) 2013, 11 (1):1 Journal of Genetic Engineering and Biotechnologyen
dc.identifier.issn1687157Xen
dc.identifier.doi10.1016/j.jgeb.2013.04.003en
dc.identifier.urihttp://hdl.handle.net/10754/552461en
dc.description.abstractBlueberry is a widely grown and easily perishable fruit crop. An efficient post-harvest handling is critical, and for that purpose gene technology methods have been part of ongoing programmes to improve crops with high food values such as blueberry. Here we report the isolation, cloning, characterization and differential expression levels of two cDNAs encoding Polygalacturonase-Inhibitor Protein (PGIP) and Cinnamoyl-Coa Reductase (CCR) from blueberry fruits in relation to various storage conditions. The open reading frame of PGIP and CCR encodes a polypeptide of 329 and 347 amino acids, respectively. To assess changes in the expression of blueberry PGIP and CCR after harvest, a storage trial was initiated. The northern blots hybridization showed a clear differential expression level of PGIP and CCR between freshly harvested and stored fruits as well as between fruits stored under various storage conditions. Although the prospects of exploiting such a strategy for crop improvement are limited, the results provide further insight into the control of the quality over the storage period at the molecular level.en
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S1687157X13000097en
dc.rightsArchived with thanks to Journal of Genetic Engineering and Biotechnology. http://creativecommons.org/licenses/by-nc-nd/3.0/en
dc.subjectBlueberryen
dc.subjectPolygalacturonase-Inhibiting Proteinen
dc.subjectCinnamoyl-Coa Reductaseen
dc.subjectGene expressionen
dc.subjectFruit storageen
dc.titleMolecular cloning and characterization of Polygalacturonase-Inhibiting Protein and Cinnamoyl-Coa Reductase genes and their association with fruit storage conditions in blueberry (Vaccinium corymbosum)en
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalJournal of Genetic Engineering and Biotechnologyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Biology and Biochemistry, Birzeit University, P.O. Box 7, Birzeit, West Bank, Palestineen
kaust.authorKhraiwesh, Baselen
kaust.authorQudeimat, Enasen
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