Fabrication and Characterization of a Microfluidic Device to Ultrapurify Blood Samples

Handle URI:
http://hdl.handle.net/10754/552418
Title:
Fabrication and Characterization of a Microfluidic Device to Ultrapurify Blood Samples
Authors:
Tallerico, Marco ( 0000-0002-6791-9068 )
Abstract:
The improvement of blood cell sorting techniques in recent years have attracted the attention of many researchers due to the possible benefits that these methods can lead in biology, regenerative medicine, materials science and therapeutic area. In this work a cell sorting technique based on filtration is described. The separation occurs by means of a microfluidic device, suitably designed, manufactured and tested, that is connected to an external experimental set-up. The fabrication process can be divided in two parts: at first it is described the manufacturing process of a filtering membrane, with holes of specific size that allow the passage of only certain cell types. Following the microfluidic device is fabricated through the mechanical micromilling. The membrane and the microdevice are suitably bonded and tested by means of an external connection with syringe pumps that inject blood samples at specific flow rates. The device is designed to separate blood cells and tumor cells only by using differences in size and shape. In particular during the first experiments red blood cells and platelets are sorted from white blood cells; in the other experiments red blood cells and platelets are separated from white blood cells and tumor cells. The microdevice has proven to be very efficient, in fact a capture efficiency of 99% is achieved. For this reason it could be used in identification and isolation of circulating tumor cells, a very rare cancer cell type whose presence in the bloodstream could be symptom of future solid tumor formation. The various experiments have also demonstrated that tumor cells survive even after the separation treatment, and then the suffered stress during the sorting process does not harm the biological sample.
Advisors:
Di Fabrizio, Enzo ( 0000-0001-5886-4678 )
Committee Member:
Traversa, Enrico ( 0000-0001-6336-941X ) ; Rothenberger, Alexander
KAUST Department:
Physical Sciences and Engineering (PSE) Division
Program:
Materials Science and Engineering
Issue Date:
4-May-2015
Type:
Thesis
Appears in Collections:
Theses; Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program

Full metadata record

DC FieldValue Language
dc.contributor.advisorDi Fabrizio, Enzoen
dc.contributor.authorTallerico, Marcoen
dc.date.accessioned2015-05-07T11:54:48Zen
dc.date.available2015-05-07T11:54:48Zen
dc.date.issued2015-05-04en
dc.identifier.urihttp://hdl.handle.net/10754/552418en
dc.description.abstractThe improvement of blood cell sorting techniques in recent years have attracted the attention of many researchers due to the possible benefits that these methods can lead in biology, regenerative medicine, materials science and therapeutic area. In this work a cell sorting technique based on filtration is described. The separation occurs by means of a microfluidic device, suitably designed, manufactured and tested, that is connected to an external experimental set-up. The fabrication process can be divided in two parts: at first it is described the manufacturing process of a filtering membrane, with holes of specific size that allow the passage of only certain cell types. Following the microfluidic device is fabricated through the mechanical micromilling. The membrane and the microdevice are suitably bonded and tested by means of an external connection with syringe pumps that inject blood samples at specific flow rates. The device is designed to separate blood cells and tumor cells only by using differences in size and shape. In particular during the first experiments red blood cells and platelets are sorted from white blood cells; in the other experiments red blood cells and platelets are separated from white blood cells and tumor cells. The microdevice has proven to be very efficient, in fact a capture efficiency of 99% is achieved. For this reason it could be used in identification and isolation of circulating tumor cells, a very rare cancer cell type whose presence in the bloodstream could be symptom of future solid tumor formation. The various experiments have also demonstrated that tumor cells survive even after the separation treatment, and then the suffered stress during the sorting process does not harm the biological sample.en
dc.language.isoenen
dc.subjectMicrofluidic Deviceen
dc.subjectBlood Samplesen
dc.subjectCTCsen
dc.subjectSyringe Pumpsen
dc.subjectUltrapurificationen
dc.subjectCell Sortingen
dc.titleFabrication and Characterization of a Microfluidic Device to Ultrapurify Blood Samplesen
dc.typeThesisen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
thesis.degree.grantorKing Abdullah University of Science and Technologyen_GB
dc.contributor.committeememberTraversa, Enricoen
dc.contributor.committeememberRothenberger, Alexanderen
thesis.degree.disciplineMaterials Science and Engineeringen
thesis.degree.nameMaster of Scienceen
dc.person.id129132en
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.