Cyclic mononucleotides modulate potassium and calcium flux responses to H2O2 in Arabidopsis roots

Handle URI:
http://hdl.handle.net/10754/552388
Title:
Cyclic mononucleotides modulate potassium and calcium flux responses to H2O2 in Arabidopsis roots
Authors:
Ordoñez, Natalia Maria; Marondedze, Claudius ( 0000-0002-2113-904X ) ; Thomas, Ludivine; Pasqualini, Stefania; Shabala, Lana; Shabala, Sergey; Gehring, Christoph A. ( 0000-0003-4355-4591 )
Abstract:
Cyclic mononucleotides are messengers in plant stress responses. Here we show that hydrogen peroxide (H2O2) induces rapid net K+-efflux and Ca2+-influx in Arabidopsis roots. Pre-treatment with either 10 μM cAMP or cGMP for 1 or 24 h does significantly reduce net K+-leakage and Ca2+-influx, and in the case of the K+-fluxes, the cell permeant cyclic mononucleotides are more effective. We also examined the effect of 10 μM of the cell permeant 8-Br-cGMP on the Arabidopsis microsomal proteome and noted a specific increase in proteins with a role in stress responses and ion transport, suggesting that cGMP is sufficient to directly and/or indirectly induce complex adaptive changes to cellular stresses induced by H2O2. © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Biosciences Core Lab
Citation:
Cyclic mononucleotides modulate potassium and calcium flux responses to H2O2 in Arabidopsis roots 2014, 588 (6):1008 FEBS Letters
Publisher:
Wiley-Blackwell
Journal:
FEBS Letters
Issue Date:
13-Feb-2014
DOI:
10.1016/j.febslet.2014.01.062
Type:
Article
ISSN:
00145793
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0014579314001057
Appears in Collections:
Articles; Biosciences Core Lab; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorOrdoñez, Natalia Mariaen
dc.contributor.authorMarondedze, Claudiusen
dc.contributor.authorThomas, Ludivineen
dc.contributor.authorPasqualini, Stefaniaen
dc.contributor.authorShabala, Lanaen
dc.contributor.authorShabala, Sergeyen
dc.contributor.authorGehring, Christoph A.en
dc.date.accessioned2015-05-06T13:31:19Zen
dc.date.available2015-05-06T13:31:19Zen
dc.date.issued2014-02-13en
dc.identifier.citationCyclic mononucleotides modulate potassium and calcium flux responses to H2O2 in Arabidopsis roots 2014, 588 (6):1008 FEBS Lettersen
dc.identifier.issn00145793en
dc.identifier.doi10.1016/j.febslet.2014.01.062en
dc.identifier.urihttp://hdl.handle.net/10754/552388en
dc.description.abstractCyclic mononucleotides are messengers in plant stress responses. Here we show that hydrogen peroxide (H2O2) induces rapid net K+-efflux and Ca2+-influx in Arabidopsis roots. Pre-treatment with either 10 μM cAMP or cGMP for 1 or 24 h does significantly reduce net K+-leakage and Ca2+-influx, and in the case of the K+-fluxes, the cell permeant cyclic mononucleotides are more effective. We also examined the effect of 10 μM of the cell permeant 8-Br-cGMP on the Arabidopsis microsomal proteome and noted a specific increase in proteins with a role in stress responses and ion transport, suggesting that cGMP is sufficient to directly and/or indirectly induce complex adaptive changes to cellular stresses induced by H2O2. © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.en
dc.publisherWiley-Blackwellen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0014579314001057en
dc.rightsArchived with thanks to FEBS Letters. http://creativecommons.org/licenses/by-nc-nd/3.0/en
dc.subjectPlant stressen
dc.subjectHydrogen peroxideen
dc.subjectCyclic mononucleotideen
dc.subjectcAMPen
dc.subjectcGMPen
dc.subjectIon fluxen
dc.subjectProteomicen
dc.subjectArabidopsis thalianaen
dc.titleCyclic mononucleotides modulate potassium and calcium flux responses to H2O2 in Arabidopsis rootsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBiosciences Core Laben
dc.identifier.journalFEBS Lettersen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Applied Biology, University of Perugia, Borgo XX Giugno, 74, 06121 Perugia, Italyen
dc.contributor.institutionSchool of Agricultural Science, University of Tasmania, Private Bag 54, Hobart, Tasmania 7001, Australiaen
kaust.authorMarondedze, Claudiusen
kaust.authorThomas, Ludivineen
kaust.authorGehring, Christoph A.en
kaust.authorOrdoñez, Natalia Mariaen
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