High-throughput particle manipulation by hydrodynamic, electrokinetic, and dielectrophoretic effects in an integrated microfluidic chip

Handle URI:
http://hdl.handle.net/10754/552301
Title:
High-throughput particle manipulation by hydrodynamic, electrokinetic, and dielectrophoretic effects in an integrated microfluidic chip
Authors:
Li, Shunbo; Li, Ming; Bougot-Robin, Kristelle; Cao, Wenbin; Yeung Yeung Chau, Irene; Li, Weihua; Wen, Weijia
Abstract:
Integrating different steps on a chip for cell manipulations and sample preparation is of foremost importance to fully take advantage of microfluidic possibilities, and therefore make tests faster, cheaper and more accurate. We demonstrated particle manipulation in an integrated microfluidic device by applying hydrodynamic, electroosmotic (EO), electrophoretic (EP), and dielectrophoretic (DEP) forces. The process involves generation of fluid flow by pressure difference, particle trapping by DEP force, and particle redirect by EO and EP forces. Both DC and AC signals were applied, taking advantages of DC EP, EO and AC DEP for on-chip particle manipulation. Since different types of particles respond differently to these signals, variations of DC and AC signals are capable to handle complex and highly variable colloidal and biological samples. The proposed technique can operate in a high-throughput manner with thirteen independent channels in radial directions for enrichment and separation in microfluidic chip. We evaluated our approach by collecting Polystyrene particles, yeast cells, and E. coli bacteria, which respond differently to electric field gradient. Live and dead yeast cells were separated successfully, validating the capability of our device to separate highly similar cells. Our results showed that this technique could achieve fast pre-concentration of colloidal particles and cells and separation of cells depending on their vitality. Hydrodynamic, DC electrophoretic and DC electroosmotic forces were used together instead of syringe pump to achieve sufficient fluid flow and particle mobility for particle trapping and sorting. By eliminating bulky mechanical pumps, this new technique has wide applications for in situ detection and analysis.
KAUST Department:
KAUST-HKUST Joint Micro/Nanofluidic Laboratory
Citation:
High-throughput particle manipulation by hydrodynamic, electrokinetic, and dielectrophoretic effects in an integrated microfluidic chip 2013, 7 (2):024106 Biomicrofluidics
Publisher:
AIP Publishing
Journal:
Biomicrofluidics
Issue Date:
20-Mar-2013
DOI:
10.1063/1.4795856
PubMed ID:
24404011
PubMed Central ID:
PMC3618097
Type:
Article
ISSN:
19321058
Additional Links:
http://scitation.aip.org/content/aip/journal/bmf/7/2/10.1063/1.4795856
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorLi, Shunboen
dc.contributor.authorLi, Mingen
dc.contributor.authorBougot-Robin, Kristelleen
dc.contributor.authorCao, Wenbinen
dc.contributor.authorYeung Yeung Chau, Ireneen
dc.contributor.authorLi, Weihuaen
dc.contributor.authorWen, Weijiaen
dc.date.accessioned2015-05-05T14:29:13Zen
dc.date.available2015-05-05T14:29:13Zen
dc.date.issued2013-03-20en
dc.identifier.citationHigh-throughput particle manipulation by hydrodynamic, electrokinetic, and dielectrophoretic effects in an integrated microfluidic chip 2013, 7 (2):024106 Biomicrofluidicsen
dc.identifier.issn19321058en
dc.identifier.pmid24404011en
dc.identifier.doi10.1063/1.4795856en
dc.identifier.urihttp://hdl.handle.net/10754/552301en
dc.description.abstractIntegrating different steps on a chip for cell manipulations and sample preparation is of foremost importance to fully take advantage of microfluidic possibilities, and therefore make tests faster, cheaper and more accurate. We demonstrated particle manipulation in an integrated microfluidic device by applying hydrodynamic, electroosmotic (EO), electrophoretic (EP), and dielectrophoretic (DEP) forces. The process involves generation of fluid flow by pressure difference, particle trapping by DEP force, and particle redirect by EO and EP forces. Both DC and AC signals were applied, taking advantages of DC EP, EO and AC DEP for on-chip particle manipulation. Since different types of particles respond differently to these signals, variations of DC and AC signals are capable to handle complex and highly variable colloidal and biological samples. The proposed technique can operate in a high-throughput manner with thirteen independent channels in radial directions for enrichment and separation in microfluidic chip. We evaluated our approach by collecting Polystyrene particles, yeast cells, and E. coli bacteria, which respond differently to electric field gradient. Live and dead yeast cells were separated successfully, validating the capability of our device to separate highly similar cells. Our results showed that this technique could achieve fast pre-concentration of colloidal particles and cells and separation of cells depending on their vitality. Hydrodynamic, DC electrophoretic and DC electroosmotic forces were used together instead of syringe pump to achieve sufficient fluid flow and particle mobility for particle trapping and sorting. By eliminating bulky mechanical pumps, this new technique has wide applications for in situ detection and analysis.en
dc.publisherAIP Publishingen
dc.relation.urlhttp://scitation.aip.org/content/aip/journal/bmf/7/2/10.1063/1.4795856en
dc.rightsArchived with thanks to Biomicrofluidicsen
dc.titleHigh-throughput particle manipulation by hydrodynamic, electrokinetic, and dielectrophoretic effects in an integrated microfluidic chipen
dc.typeArticleen
dc.contributor.departmentKAUST-HKUST Joint Micro/Nanofluidic Laboratoryen
dc.identifier.journalBiomicrofluidicsen
dc.identifier.pmcidPMC3618097en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Physics and KAUST-HKUST joint Micro/Nanofluidic Laboratory, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kongen
dc.contributor.institutionSchool of Mechanical, Materials and Mechatronic Engineering, University of Wollongong, Wollongong 2522, New South Wales, Australiaen
dc.contributor.institutionInstitute for Advanced Study, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kongen
dc.contributor.institutionNano Science and Technology Program and Department of Physics, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kongen
kaust.authorLi, Shunboen
kaust.authorWen, Weijiaen

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