Nkx2.1-derived astrocytes and neurons together with Slit2 are indispensable for anterior commissure formation

Handle URI:
http://hdl.handle.net/10754/551010
Title:
Nkx2.1-derived astrocytes and neurons together with Slit2 are indispensable for anterior commissure formation
Authors:
Minocha, Shilpi; Valloton, Delphine; Ypsilanti, Athena R.; Fiumelli, Hubert ( 0000-0001-5136-3045 ) ; Allen, Elizabeth A.; Yanagawa, Yuchio; Marin, Oscar; Chédotal, Alain ( 0000-0001-7577-3794 ) ; Hornung, Jean-Pierre ( 0000-0002-9229-7520 ) ; Lebrand, Cécile
Abstract:
Guidepost cells present at and surrounding the midline provide guidance cues that orient the growing axons through commissures. Here we show that the transcription factor Nkx2.1 known to control the specification of GABAergic interneurons also regulates the differentiation of astroglia and polydendrocytes within the mouse anterior commissure (AC). Nkx2.1-positive glia were found to originate from three germinal regions of the ventral telencephalon. Nkx2.1-derived glia were observed in and around the AC region by E14.5. Thereafter, a selective cell ablation strategy showed a synergistic role of Nkx2.1-derived cells, both GABAergic interneurons and astroglia, towards the proper formation of the AC. Finally, our results reveal that the Nkx2.1-regulated cells mediate AC axon guidance through the expression of the repellent cue, Slit2. These results bring forth interesting insights about the spatial and temporal origin of midline telencephalic glia, and highlight the importance of neurons and astroglia towards the formation of midline commissures.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Nkx2.1-derived astrocytes and neurons together with Slit2 are indispensable for anterior commissure formation 2015, 6:6887 Nature Communications
Journal:
Nature Communications
Issue Date:
23-Apr-2015
DOI:
10.1038/ncomms7887
PubMed ID:
25904499
PubMed Central ID:
PMC4423212
Type:
Article
ISSN:
2041-1723
Additional Links:
http://www.nature.com/doifinder/10.1038/ncomms7887
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMinocha, Shilpien
dc.contributor.authorValloton, Delphineen
dc.contributor.authorYpsilanti, Athena R.en
dc.contributor.authorFiumelli, Huberten
dc.contributor.authorAllen, Elizabeth A.en
dc.contributor.authorYanagawa, Yuchioen
dc.contributor.authorMarin, Oscaren
dc.contributor.authorChédotal, Alainen
dc.contributor.authorHornung, Jean-Pierreen
dc.contributor.authorLebrand, Cécileen
dc.date.accessioned2015-04-30T12:12:43Zen
dc.date.available2015-04-30T12:12:43Zen
dc.date.issued2015-04-23en
dc.identifier.citationNkx2.1-derived astrocytes and neurons together with Slit2 are indispensable for anterior commissure formation 2015, 6:6887 Nature Communicationsen
dc.identifier.issn2041-1723en
dc.identifier.pmid25904499en
dc.identifier.doi10.1038/ncomms7887en
dc.identifier.urihttp://hdl.handle.net/10754/551010en
dc.description.abstractGuidepost cells present at and surrounding the midline provide guidance cues that orient the growing axons through commissures. Here we show that the transcription factor Nkx2.1 known to control the specification of GABAergic interneurons also regulates the differentiation of astroglia and polydendrocytes within the mouse anterior commissure (AC). Nkx2.1-positive glia were found to originate from three germinal regions of the ventral telencephalon. Nkx2.1-derived glia were observed in and around the AC region by E14.5. Thereafter, a selective cell ablation strategy showed a synergistic role of Nkx2.1-derived cells, both GABAergic interneurons and astroglia, towards the proper formation of the AC. Finally, our results reveal that the Nkx2.1-regulated cells mediate AC axon guidance through the expression of the repellent cue, Slit2. These results bring forth interesting insights about the spatial and temporal origin of midline telencephalic glia, and highlight the importance of neurons and astroglia towards the formation of midline commissures.en
dc.relation.urlhttp://www.nature.com/doifinder/10.1038/ncomms7887en
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.subjectBiological sciencesen
dc.subjectNeuroscienceen
dc.subjectDevelopmental biologyen
dc.titleNkx2.1-derived astrocytes and neurons together with Slit2 are indispensable for anterior commissure formationen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalNature Communicationsen
dc.identifier.pmcidPMC4423212en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Fundamental Neurosciences, University of Lausanne, Lausanne CH-1005, Switzerlanden
dc.contributor.institutionINSERM UMR-S968, Institut de la Vision, Paris F-75012, Franceen
dc.contributor.institutionSorbonne Université, UPMC University Paris 06, UMR_S 968, Institut de la Vision, Paris F-75012, Franceen
dc.contributor.institutionCNRS, UMR_7210, Paris F-75012, Franceen
dc.contributor.institutionEPFL, Station 19, Batiment-SV, Room SV-2816, Lausanne CH-1015, Switzerlanden
dc.contributor.institutionDepartment of Genetic and Behavioral Neuroscience, Gunma University Graduate School of Medicine and Japan Science and Technology Agency, CREST, Maebashi 371-8511, Japanen
dc.contributor.institutionInstituto de Neurociencias, Consejo Superior de Investigaciones Científicas and Universidad Miguel Hernández, Sant Joan d’Alacant 03550, Spainen
kaust.authorFiumelli, Huberten

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