Carotenoid metabolic profiling and transcriptome-genome mining reveal functional equivalence among blue-pigmented copepods and appendicularia

Handle URI:
http://hdl.handle.net/10754/550807
Title:
Carotenoid metabolic profiling and transcriptome-genome mining reveal functional equivalence among blue-pigmented copepods and appendicularia
Authors:
Mojib, Nazia ( 0000-0003-4924-5538 ) ; Amad, Maan H.; Thimma, Manjula; Aldanondo, Naroa; Kumaran, Mande; Irigoien, Xabier ( 0000-0002-5411-6741 )
Abstract:
The tropical oligotrophic oceanic areas are characterized by high water transparency and annual solar radiation. Under these conditions, a large number of phylogenetically diverse mesozooplankton species living in the surface waters (neuston) are found to be blue pigmented. In the present study, we focused on understanding the metabolic and genetic basis of the observed blue phenotype functional equivalence between the blue-pigmented organisms from the phylum Arthropoda, subclass Copepoda (Acartia fossae) and the phylum Chordata, class Appendicularia (Oikopleura dioica) in the Red Sea. Previous studies have shown that carotenoid–protein complexes are responsible for blue coloration in crustaceans. Therefore, we performed carotenoid metabolic profiling using both targeted and nontargeted (high-resolution mass spectrometry) approaches in four different blue-pigmented genera of copepods and one blue-pigmented species of appendicularia. Astaxanthin was found to be the principal carotenoid in all the species. The pathway analysis showed that all the species can synthesize astaxanthin from β-carotene, ingested from dietary sources, via 3-hydroxyechinenone, canthaxanthin, zeaxanthin, adonirubin or adonixanthin. Further, using de novo assembled transcriptome of blue A. fossae (subclass Copepoda), we identified highly expressed homologous β-carotene hydroxylase enzymes and putative carotenoid-binding proteins responsible for astaxanthin formation and the blue phenotype. In blue O. dioica (class Appendicularia), corresponding putative genes were identified from the reference genome. Collectively, our data provide molecular evidences for the bioconversion and accumulation of blue astaxanthin–protein complexes underpinning the observed ecological functional equivalence and adaptive convergence among neustonic mesozooplankton.
KAUST Department:
Red Sea Research Center (RSRC); Analytical Core Lab; Biosciences Core Lab
Citation:
Carotenoid metabolic profiling and transcriptome-genome mining reveal functional equivalence among blue-pigmented copepods and appendicularia 2014, 23 (11):2740 Molecular Ecology
Publisher:
Wiley
Journal:
Molecular Ecology
Issue Date:
Jun-2014
DOI:
10.1111/mec.12781
Type:
Article
ISSN:
09621083
Additional Links:
http://doi.wiley.com/10.1111/mec.12781
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Analytical Core Lab; Biosciences Core Lab

Full metadata record

DC FieldValue Language
dc.contributor.authorMojib, Naziaen
dc.contributor.authorAmad, Maan H.en
dc.contributor.authorThimma, Manjulaen
dc.contributor.authorAldanondo, Naroaen
dc.contributor.authorKumaran, Mandeen
dc.contributor.authorIrigoien, Xabieren
dc.date.accessioned2015-04-28T08:45:29Zen
dc.date.available2015-04-28T08:45:29Zen
dc.date.issued2014-06en
dc.identifier.citationCarotenoid metabolic profiling and transcriptome-genome mining reveal functional equivalence among blue-pigmented copepods and appendicularia 2014, 23 (11):2740 Molecular Ecologyen
dc.identifier.issn09621083en
dc.identifier.doi10.1111/mec.12781en
dc.identifier.urihttp://hdl.handle.net/10754/550807en
dc.description.abstractThe tropical oligotrophic oceanic areas are characterized by high water transparency and annual solar radiation. Under these conditions, a large number of phylogenetically diverse mesozooplankton species living in the surface waters (neuston) are found to be blue pigmented. In the present study, we focused on understanding the metabolic and genetic basis of the observed blue phenotype functional equivalence between the blue-pigmented organisms from the phylum Arthropoda, subclass Copepoda (Acartia fossae) and the phylum Chordata, class Appendicularia (Oikopleura dioica) in the Red Sea. Previous studies have shown that carotenoid–protein complexes are responsible for blue coloration in crustaceans. Therefore, we performed carotenoid metabolic profiling using both targeted and nontargeted (high-resolution mass spectrometry) approaches in four different blue-pigmented genera of copepods and one blue-pigmented species of appendicularia. Astaxanthin was found to be the principal carotenoid in all the species. The pathway analysis showed that all the species can synthesize astaxanthin from β-carotene, ingested from dietary sources, via 3-hydroxyechinenone, canthaxanthin, zeaxanthin, adonirubin or adonixanthin. Further, using de novo assembled transcriptome of blue A. fossae (subclass Copepoda), we identified highly expressed homologous β-carotene hydroxylase enzymes and putative carotenoid-binding proteins responsible for astaxanthin formation and the blue phenotype. In blue O. dioica (class Appendicularia), corresponding putative genes were identified from the reference genome. Collectively, our data provide molecular evidences for the bioconversion and accumulation of blue astaxanthin–protein complexes underpinning the observed ecological functional equivalence and adaptive convergence among neustonic mesozooplankton.en
dc.publisherWileyen
dc.relation.urlhttp://doi.wiley.com/10.1111/mec.12781en
dc.rightsThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.en
dc.subjectastaxanthinen
dc.subjectcarotenoid-binding proteinsen
dc.subjecthigh-resolution mass spectrometryen
dc.subjectmesozooplanktonen
dc.subjecttranscriptomeen
dc.subjectβ-carotene hydroxylaseen
dc.titleCarotenoid metabolic profiling and transcriptome-genome mining reveal functional equivalence among blue-pigmented copepods and appendiculariaen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentAnalytical Core Laben
dc.contributor.departmentBiosciences Core Laben
dc.identifier.journalMolecular Ecologyen
dc.eprint.versionPublisher's Version/PDFen
kaust.authorMojib, Naziaen
kaust.authorAmad, Maan H.en
kaust.authorThimma, Manjulaen
kaust.authorAldanondo, Naroaen
kaust.authorIrigoien, Xabieren
kaust.authorKumaran, Mandeen
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.