Dynamic changes in the date palm fruit proteome during development and ripening

Handle URI:
http://hdl.handle.net/10754/550677
Title:
Dynamic changes in the date palm fruit proteome during development and ripening
Authors:
Marondedze, Claudius ( 0000-0002-2113-904X ) ; Gehring, Christoph A. ( 0000-0003-4355-4591 ) ; Thomas, Ludivine
Abstract:
Date palm (Phoenix dactylifera) is an economically important fruit tree in the Middle East and North Africa and is characterized by large cultivar diversity, making it a good model for studies on fruit development and other important traits. Here in gel comparative proteomics combined with tandem mass spectrometry were used to study date fruit development and ripening. Total proteins were extracted using a phenol-based protocol. A total of 189 protein spots were differentially regulated (p≤0.05). The identified proteins were classified into 14 functional categories. The categories with the most proteins were ‘disease and defense’ (16.5%) and ‘metabolism’ (15.4%). Twenty-nine proteins have not previously been identified in other fleshy fruits and 64 showed contrasting expression patterns in other fruits. Abundance of most proteins with a role in abiotic stress responses increased during ripening with the exception of heat shock proteins. Proteins with a role in anthocyanin biosynthesis, glycolysis, tricarboxylic acid cycle and cell wall degradation were upregulated particularly from the onset of ripening and during ripening. In contrast, expression of pentose phosphate- and photosynthesis-related proteins decreased during fruit maturation. Although date palm is considered a climacteric species, the analysis revealed downregulation of two enzymes involved in ethylene biosynthesis, suggesting an ethylene-independent ripening of ‘Barhi’ fruits. In summary, this proteomics study provides insights into physiological processes during date fruit development and ripening at the systems level and offers a reference proteome for the study of regulatory mechanisms that can inform molecular and biotechnological approaches to further improvements of horticultural traits including fruit quality and yield.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience and Bioengineering Core Facility
Citation:
Dynamic changes in the date palm fruit proteome during development and ripening 2014, 1:14039 Horticulture Research
Publisher:
Nature Publishing Group
Journal:
Horticulture Research
Issue Date:
6-Aug-2014
DOI:
10.1038/hortres.2014.39
Type:
Article
ISSN:
2052-7276
Additional Links:
http://www.nature.com/articles/hortres201439
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMarondedze, Claudiusen
dc.contributor.authorGehring, Christoph A.en
dc.contributor.authorThomas, Ludivineen
dc.date.accessioned2015-04-26T14:09:08Zen
dc.date.available2015-04-26T14:09:08Zen
dc.date.issued2014-08-06en
dc.identifier.citationDynamic changes in the date palm fruit proteome during development and ripening 2014, 1:14039 Horticulture Researchen
dc.identifier.issn2052-7276en
dc.identifier.doi10.1038/hortres.2014.39en
dc.identifier.urihttp://hdl.handle.net/10754/550677en
dc.description.abstractDate palm (Phoenix dactylifera) is an economically important fruit tree in the Middle East and North Africa and is characterized by large cultivar diversity, making it a good model for studies on fruit development and other important traits. Here in gel comparative proteomics combined with tandem mass spectrometry were used to study date fruit development and ripening. Total proteins were extracted using a phenol-based protocol. A total of 189 protein spots were differentially regulated (p≤0.05). The identified proteins were classified into 14 functional categories. The categories with the most proteins were ‘disease and defense’ (16.5%) and ‘metabolism’ (15.4%). Twenty-nine proteins have not previously been identified in other fleshy fruits and 64 showed contrasting expression patterns in other fruits. Abundance of most proteins with a role in abiotic stress responses increased during ripening with the exception of heat shock proteins. Proteins with a role in anthocyanin biosynthesis, glycolysis, tricarboxylic acid cycle and cell wall degradation were upregulated particularly from the onset of ripening and during ripening. In contrast, expression of pentose phosphate- and photosynthesis-related proteins decreased during fruit maturation. Although date palm is considered a climacteric species, the analysis revealed downregulation of two enzymes involved in ethylene biosynthesis, suggesting an ethylene-independent ripening of ‘Barhi’ fruits. In summary, this proteomics study provides insights into physiological processes during date fruit development and ripening at the systems level and offers a reference proteome for the study of regulatory mechanisms that can inform molecular and biotechnological approaches to further improvements of horticultural traits including fruit quality and yield.en
dc.publisherNature Publishing Groupen
dc.relation.urlhttp://www.nature.com/articles/hortres201439en
dc.rightsThis work is licensed under a Creative Commons Attribution 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/en
dc.titleDynamic changes in the date palm fruit proteome during development and ripeningen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience and Bioengineering Core Facilityen
dc.identifier.journalHorticulture Researchen
dc.eprint.versionPublisher's Version/PDFen
kaust.authorMarondedze, Claudiusen
kaust.authorGehring, Christoph A.en
kaust.authorThomas, Ludivineen
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