Zooplankton diversity across three Red Sea reefs using pyrosequencing

Handle URI:
http://hdl.handle.net/10754/550662
Title:
Zooplankton diversity across three Red Sea reefs using pyrosequencing
Authors:
Pearman, John K. ( 0000-0002-2237-9723 ) ; El-Sherbiny, Mohsen M.; Lanzén, Anders; Al-Aidaroos, Ali M.; Irigoien, Xabier ( 0000-0002-5411-6741 )
Abstract:
Coral reefs are considered among the most diverse ecosystems on Earth, yet little is known about the diversity of plankton in the surrounding water column. Moreover, few studies have utilized genomic methods to investigate zooplankton diversity in any habitat. This study investigated the diversity of taxa by sampling 45 stations around three reef systems in the central/southern Red Sea. The diversity of metazoan plankton was investigated by targeting the 18S rRNA gene and clustering OTUs at 97% sequence similarity. A total of 754 and 854 metazoan OTUs were observed in the data set for the 1380F and 1389F primer sets respectively. The phylum Arthropoda dominated both primer sets accounting for ~60% of reads followed by Cnidaria (~20%). Only about 20% of OTUs were shared between all three reef systems and the relation between geographic distance and Jaccard Similarity measures was not significant. Cluster analysis showed that there was no distinct split between reefs and stations from different reefs clustered together both for metazoans as a whole and for the phyla Arthropoda, Cnidaria and Chordata separately. This suggests that distance may not be a determining factor in the taxonomic composition of stations.
KAUST Department:
Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Zooplankton diversity across three Red Sea reefs using pyrosequencing 2014, 1 Frontiers in Marine Science
Publisher:
Frontiers Media SA
Journal:
Frontiers in Marine Science
Issue Date:
30-Jul-2014
DOI:
10.3389/fmars.2014.00027
Type:
Article
ISSN:
2296-7745
Additional Links:
http://journal.frontiersin.org/article/10.3389/fmars.2014.00027/abstract
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorPearman, John K.en
dc.contributor.authorEl-Sherbiny, Mohsen M.en
dc.contributor.authorLanzén, Andersen
dc.contributor.authorAl-Aidaroos, Ali M.en
dc.contributor.authorIrigoien, Xabieren
dc.date.accessioned2015-04-26T14:06:35Zen
dc.date.available2015-04-26T14:06:35Zen
dc.date.issued2014-07-30en
dc.identifier.citationZooplankton diversity across three Red Sea reefs using pyrosequencing 2014, 1 Frontiers in Marine Scienceen
dc.identifier.issn2296-7745en
dc.identifier.doi10.3389/fmars.2014.00027en
dc.identifier.urihttp://hdl.handle.net/10754/550662en
dc.description.abstractCoral reefs are considered among the most diverse ecosystems on Earth, yet little is known about the diversity of plankton in the surrounding water column. Moreover, few studies have utilized genomic methods to investigate zooplankton diversity in any habitat. This study investigated the diversity of taxa by sampling 45 stations around three reef systems in the central/southern Red Sea. The diversity of metazoan plankton was investigated by targeting the 18S rRNA gene and clustering OTUs at 97% sequence similarity. A total of 754 and 854 metazoan OTUs were observed in the data set for the 1380F and 1389F primer sets respectively. The phylum Arthropoda dominated both primer sets accounting for ~60% of reads followed by Cnidaria (~20%). Only about 20% of OTUs were shared between all three reef systems and the relation between geographic distance and Jaccard Similarity measures was not significant. Cluster analysis showed that there was no distinct split between reefs and stations from different reefs clustered together both for metazoans as a whole and for the phyla Arthropoda, Cnidaria and Chordata separately. This suggests that distance may not be a determining factor in the taxonomic composition of stations.en
dc.publisherFrontiers Media SAen
dc.relation.urlhttp://journal.frontiersin.org/article/10.3389/fmars.2014.00027/abstracten
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.titleZooplankton diversity across three Red Sea reefs using pyrosequencingen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalFrontiers in Marine Scienceen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Marine Biology, Faculty of Marine Science, King Abdelaziz University, Jeddah, Kingdom of Saudi Arabiaen
dc.contributor.institutionMarine Science Department, Faculty of Marine Science, Suez Canal University, Ismailia, Egypten
dc.contributor.institutionDepartment of Ecology and Natural Resources, NEIKER-Tecnalia, Derio, Spainen
kaust.authorPearman, John K.en
kaust.authorIrigoien, Xabieren
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