Functional analysis of Arabidopsis immune-related MAPKs uncovers a role for MPK3 as negative regulator of inducible defences

Handle URI:
http://hdl.handle.net/10754/550222
Title:
Functional analysis of Arabidopsis immune-related MAPKs uncovers a role for MPK3 as negative regulator of inducible defences
Authors:
Frei dit Frey, Nicolas; Garcia, Ana; Bigeard, Jean; Zaag, Rim; Bueso, Eduardo; Garmier, Marie; Pateyron, Stéphanie; de Tauzia-Moreau, Marie-Ludivine; Brunaud, Véronique; Balzergue, Sandrine; Colcombet, Jean; Aubourg, Sébastien; Martin-Magniette, Marie-Laure; Hirt, Heribert ( 0000-0003-3119-9633 )
Abstract:
Background Mitogen-activated protein kinases (MAPKs) are key regulators of immune responses in animals and plants. In Arabidopsis, perception of microbe-associated molecular patterns (MAMPs) activates the MAPKs MPK3, MPK4 and MPK6. Increasing information depicts the molecular events activated by MAMPs in plants, but the specific and cooperative contributions of the MAPKs in these signalling events are largely unclear. Results In this work, we analyse the behaviour of MPK3, MPK4 and MPK6 mutants in early and late immune responses triggered by the MAMP flg22 from bacterial flagellin. A genome-wide transcriptome analysis reveals that 36% of the flg22-upregulated genes and 68% of the flg22-downregulated genes are affected in at least one MAPK mutant. So far MPK4 was considered as a negative regulator of immunity, whereas MPK3 and MPK6 were believed to play partially redundant positive functions in defence. Our work reveals that MPK4 is required for the regulation of approximately 50% of flg22-induced genes and we identify a negative role for MPK3 in regulating defence gene expression, flg22-induced salicylic acid accumulation and disease resistance to Pseudomonas syringae. Among the MAPK-dependent genes, 27% of flg22-upregulated genes and 76% of flg22-downregulated genes require two or three MAPKs for their regulation. The flg22-induced MAPK activities are differentially regulated in MPK3 and MPK6 mutants, both in amplitude and duration, revealing a highly interdependent network. Conclusions These data reveal a new set of distinct functions for MPK3, MPK4 and MPK6 and indicate that the plant immune signalling network is choreographed through the interplay of these three interwoven MAPK pathways.
KAUST Department:
Center for Desert Agriculture
Citation:
Functional analysis of Arabidopsis immune-related MAPKs uncovers a role for MPK3 as negative regulator of inducible defences 2014, 15 (6):R87 Genome Biology
Publisher:
Springer Nature
Journal:
Genome Biology
Issue Date:
30-Jun-2014
DOI:
10.1186/gb-2014-15-6-r87
PubMed ID:
24980080
PubMed Central ID:
PMC4197828
Type:
Article
ISSN:
1465-6906
Additional Links:
http://genomebiology.com/2014/15/6/R87
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorFrei dit Frey, Nicolasen
dc.contributor.authorGarcia, Anaen
dc.contributor.authorBigeard, Jeanen
dc.contributor.authorZaag, Rimen
dc.contributor.authorBueso, Eduardoen
dc.contributor.authorGarmier, Marieen
dc.contributor.authorPateyron, Stéphanieen
dc.contributor.authorde Tauzia-Moreau, Marie-Ludivineen
dc.contributor.authorBrunaud, Véroniqueen
dc.contributor.authorBalzergue, Sandrineen
dc.contributor.authorColcombet, Jeanen
dc.contributor.authorAubourg, Sébastienen
dc.contributor.authorMartin-Magniette, Marie-Laureen
dc.contributor.authorHirt, Heriberten
dc.date.accessioned2015-04-16T14:04:30Zen
dc.date.available2015-04-16T14:04:30Zen
dc.date.issued2014-06-30en
dc.identifier.citationFunctional analysis of Arabidopsis immune-related MAPKs uncovers a role for MPK3 as negative regulator of inducible defences 2014, 15 (6):R87 Genome Biologyen
dc.identifier.issn1465-6906en
dc.identifier.pmid24980080en
dc.identifier.doi10.1186/gb-2014-15-6-r87en
dc.identifier.urihttp://hdl.handle.net/10754/550222en
dc.description.abstractBackground Mitogen-activated protein kinases (MAPKs) are key regulators of immune responses in animals and plants. In Arabidopsis, perception of microbe-associated molecular patterns (MAMPs) activates the MAPKs MPK3, MPK4 and MPK6. Increasing information depicts the molecular events activated by MAMPs in plants, but the specific and cooperative contributions of the MAPKs in these signalling events are largely unclear. Results In this work, we analyse the behaviour of MPK3, MPK4 and MPK6 mutants in early and late immune responses triggered by the MAMP flg22 from bacterial flagellin. A genome-wide transcriptome analysis reveals that 36% of the flg22-upregulated genes and 68% of the flg22-downregulated genes are affected in at least one MAPK mutant. So far MPK4 was considered as a negative regulator of immunity, whereas MPK3 and MPK6 were believed to play partially redundant positive functions in defence. Our work reveals that MPK4 is required for the regulation of approximately 50% of flg22-induced genes and we identify a negative role for MPK3 in regulating defence gene expression, flg22-induced salicylic acid accumulation and disease resistance to Pseudomonas syringae. Among the MAPK-dependent genes, 27% of flg22-upregulated genes and 76% of flg22-downregulated genes require two or three MAPKs for their regulation. The flg22-induced MAPK activities are differentially regulated in MPK3 and MPK6 mutants, both in amplitude and duration, revealing a highly interdependent network. Conclusions These data reveal a new set of distinct functions for MPK3, MPK4 and MPK6 and indicate that the plant immune signalling network is choreographed through the interplay of these three interwoven MAPK pathways.en
dc.publisherSpringer Natureen
dc.relation.urlhttp://genomebiology.com/2014/15/6/R87en
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en
dc.titleFunctional analysis of Arabidopsis immune-related MAPKs uncovers a role for MPK3 as negative regulator of inducible defencesen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalGenome Biologyen
dc.identifier.pmcidPMC4197828en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionUnité de Recherche en Génomique Végétale (URGV), UMR INRA 1165 - Université d’Evry Val d’Essonne - ERL CNRS 8196 - Saclay Plant Sciences, 2 rue Gaston Crémieux, Evry 91057, Franceen
dc.contributor.institutionPresent address: Laboratoire de Recherche en Sciences Végétales (LRSV), UMR 5546, Université Paul Sabatier/CNRS, 24, chemin de Borde Rouge B.P. 42617 Auzeville, Castanet-Tolosan 31326, Franceen
dc.contributor.institutionInstitut de Biologie des Plantes (IBP), CNRS-Université Paris-Sud - UMR 8618 - Saclay Plant Sciences, Orsay, Cedex 91405, Franceen
dc.contributor.institutionUnité de Recherche en Génomique Végétale (URGV), Plateforme Transcriptome, UMR INRA 1165 - Université d’Evry Val d’Essonne - ERL CNRS 8196, 2 rue Gaston Crémieux, Evry 91057, Franceen
dc.contributor.institutionAgroParisTech, UMR 518 MIA, Paris 75005, Franceen
dc.contributor.institutionINRA, UMR 518 MIA, Paris 75005, Franceen
kaust.authorHirt, Heriberten

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