The Mode of Action of Isocyanide in Three Aquatic Organisms, Balanus amphitrite, Bugula neritina and Danio rerio

Handle URI:
http://hdl.handle.net/10754/550218
Title:
The Mode of Action of Isocyanide in Three Aquatic Organisms, Balanus amphitrite, Bugula neritina and Danio rerio
Authors:
Zhang, Yi-Fan; Kitano, Yoshikazu; Nogata, Yasuyuki; Zhang, Yu; Qian, Pei-Yuan
Abstract:
Isocyanide is a potential antifouling compound in marine environments. In this study, we investigated its mode of action in three aquatic organisms. Two of them, the bryozoan Bugula neritina and the barnacle Balanus amphitrite, are major marine fouling invertebrates, and the other organism is the non-target species zebrafish Danio rerio. In the swimming larvae of B. neritina, isocyanide did not affect the total attachment rate (≤50 µg ml^(−1)), but it did change the attachment site by increasing the percentage of attachment on the bottom of the container rather than on the wall or air-water inter-surface. Isocyanide binds several proteins in B. neritina as identified via SDS-PAGE-LC-MS/MS: 1) a 30 kD protein band containing two proteins similar to voltage dependent anion channels (VDAC), which control the direct coupling of the mitochondrial matrix to the energy maintenance of the cytosol and the release of apoptogenic factors from mitochondria of mammalian cells; and 2) an unknown 39 kD protein. In B. amphitrite cyprids, the isocyanide binding protein were 1) a protein similar to NADH-ubiquinone oxidoreductase, which is the “entry enzyme” of oxidative phosphorylation in mitochondria; and 2) cytochrome P450. In Danio rerio embryos, isocyanide caused “wavy” notochords, hydrocephalus, pericardial edema, poor blood circulation, and defects in pigmentation and hematopoiesis, which phenocopied copper deficiency. This is the first report on isocyanide binding proteins in fouling organisms, as well as the first description of its phenotype and potential toxicology in zebrafish.
Citation:
The Mode of Action of Isocyanide in Three Aquatic Organisms, Balanus amphitrite, Bugula neritina and Danio rerio 2012, 7 (9):e45442 PLoS ONE
Publisher:
Public Library of Science (PLoS)
Journal:
PLoS ONE
KAUST Grant Number:
SA-C0040; UK-C0016
Issue Date:
18-Sep-2012
DOI:
10.1371/journal.pone.0045442
PubMed ID:
23029013
PubMed Central ID:
PMC3445549
Type:
Article
ISSN:
1932-6203
Sponsors:
This study was supported by a research grant (DY125-15-T-02) from China Ocean Mineral Resources Research and Development Association, an award from King Abdullah University of Science and Technology (SA-C0040/UK-C0016) and grants from the Research Grants Council of the Hong Kong Special Administrative Region (N_HKUST602/09 and AoE/P-04/04-II) to Pei-Yuan Qian. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Additional Links:
http://dx.plos.org/10.1371/journal.pone.0045442
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorZhang, Yi-Fanen
dc.contributor.authorKitano, Yoshikazuen
dc.contributor.authorNogata, Yasuyukien
dc.contributor.authorZhang, Yuen
dc.contributor.authorQian, Pei-Yuanen
dc.date.accessioned2015-04-16T13:59:12Zen
dc.date.available2015-04-16T13:59:12Zen
dc.date.issued2012-09-18en
dc.identifier.citationThe Mode of Action of Isocyanide in Three Aquatic Organisms, Balanus amphitrite, Bugula neritina and Danio rerio 2012, 7 (9):e45442 PLoS ONEen
dc.identifier.issn1932-6203en
dc.identifier.pmid23029013en
dc.identifier.doi10.1371/journal.pone.0045442en
dc.identifier.urihttp://hdl.handle.net/10754/550218en
dc.description.abstractIsocyanide is a potential antifouling compound in marine environments. In this study, we investigated its mode of action in three aquatic organisms. Two of them, the bryozoan Bugula neritina and the barnacle Balanus amphitrite, are major marine fouling invertebrates, and the other organism is the non-target species zebrafish Danio rerio. In the swimming larvae of B. neritina, isocyanide did not affect the total attachment rate (≤50 µg ml^(−1)), but it did change the attachment site by increasing the percentage of attachment on the bottom of the container rather than on the wall or air-water inter-surface. Isocyanide binds several proteins in B. neritina as identified via SDS-PAGE-LC-MS/MS: 1) a 30 kD protein band containing two proteins similar to voltage dependent anion channels (VDAC), which control the direct coupling of the mitochondrial matrix to the energy maintenance of the cytosol and the release of apoptogenic factors from mitochondria of mammalian cells; and 2) an unknown 39 kD protein. In B. amphitrite cyprids, the isocyanide binding protein were 1) a protein similar to NADH-ubiquinone oxidoreductase, which is the “entry enzyme” of oxidative phosphorylation in mitochondria; and 2) cytochrome P450. In Danio rerio embryos, isocyanide caused “wavy” notochords, hydrocephalus, pericardial edema, poor blood circulation, and defects in pigmentation and hematopoiesis, which phenocopied copper deficiency. This is the first report on isocyanide binding proteins in fouling organisms, as well as the first description of its phenotype and potential toxicology in zebrafish.en
dc.description.sponsorshipThis study was supported by a research grant (DY125-15-T-02) from China Ocean Mineral Resources Research and Development Association, an award from King Abdullah University of Science and Technology (SA-C0040/UK-C0016) and grants from the Research Grants Council of the Hong Kong Special Administrative Region (N_HKUST602/09 and AoE/P-04/04-II) to Pei-Yuan Qian. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.publisherPublic Library of Science (PLoS)en
dc.relation.urlhttp://dx.plos.org/10.1371/journal.pone.0045442en
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.titleThe Mode of Action of Isocyanide in Three Aquatic Organisms, Balanus amphitrite, Bugula neritina and Danio rerioen
dc.typeArticleen
dc.identifier.journalPLoS ONEen
dc.identifier.pmcidPMC3445549en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDivision of Life Science, Hong Kong University of Science and Technology, Hong Kong SAR, Chinaen
dc.contributor.institutionLaboratory of Bio-organic Chemistry, Tokyo University of Agriculture and Technology, Tokyo, Japanen
dc.contributor.institutionAbiko Research Laboratory, Central Research Institute of Electric Power Industry, Chiba, Japanen
kaust.authorZhang, Yi-Fanen
kaust.authorZhang, Yuen
kaust.authorQian, Pei-Yuanen
kaust.grant.numberSA-C0040en
kaust.grant.numberUK-C0016en
kaust.grant.programKAUST Global Collaborative Research Programen
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