Evidence for the Involvement of p38 MAPK Activation in Barnacle Larval Settlement

Handle URI:
http://hdl.handle.net/10754/550215
Title:
Evidence for the Involvement of p38 MAPK Activation in Barnacle Larval Settlement
Authors:
He, Li-Sheng; Xu, Ying; Matsumura, Kiyotaka; Zhang, Yu; Zhang, Gen; Qi, Shu-Hua; Qian, Pei-Yuan
Abstract:
The barnacle Balanus ( = Amphibalanus) amphitrite is a major marine fouling animal. Understanding the molecular mechanism of larval settlement in this species is critical for anti-fouling research. In this study, we cloned one isoform of p38 MAPK (Bar-p38 MAPK) from this species, which shares the significant characteristic of containing a TGY motif with other species such as yeast, Drosophila and humans. The activation of p38 MAPK was detected by an antibody that recognizes the conserved dual phosphorylation sites of TGY. The results showed that phospho-p38 MAPK (pp38 MAPK) was more highly expressed at the cyprid stage, particularly in aged cyprids, in comparison to other stages, including the nauplius and juvenile stages. Immunostaining showed that Bar-p38 MAPK and pp38 MAPK were mainly located at the cyprid antennules, and especially the third and fourth segments, which are responsible for substratum exploration during settlement. The expression and localization patterns of Bar-p38 MAPK suggest its involvement in larval settlement. This postulation was also supported by the larval settlement bioassay with the p38 MAPK inhibitor SB203580. Behavioral analysis by live imaging revealed that the larvae were still capable of exploring the surface of the substratum after SB203580 treatment. This shows that the effect of p38 MAPK on larval settlement might be by regulating the secretion of permanent proteinaceous substances. Furthermore, the level of pp38 MAPK dramatically decreased after full settlement, suggesting that Bar-p38 MAPK maybe plays a role in larval settlement rather than metamorphosis. Finally, we found that Bar-p38 MAPK was highly activated when larvae confronted extracts of adult barnacle containing settlement cues, whereas larvae pre-treated with SB203580 failed to respond to the crude adult extracts.
Citation:
Evidence for the Involvement of p38 MAPK Activation in Barnacle Larval Settlement 2012, 7 (10):e47195 PLoS ONE
Journal:
PLoS ONE
KAUST Grant Number:
SA-C0040; UK-C0016
Issue Date:
24-Oct-2012
DOI:
10.1371/journal.pone.0047195
PubMed ID:
23115639
PubMed Central ID:
PMC3480373
Type:
Article
ISSN:
1932-6203
Sponsors:
This work was supported by an award from the King Abdullah University of Science and Technology (SA-C0040/UK-C0016) and grants (N-HKUST602/09 and AoE/P-04/04-II) from the Research Grants Council of the Hong Kong Special Administrative Region to PY Qian. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Additional Links:
http://dx.plos.org/10.1371/journal.pone.0047195
Appears in Collections:
Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorHe, Li-Shengen
dc.contributor.authorXu, Yingen
dc.contributor.authorMatsumura, Kiyotakaen
dc.contributor.authorZhang, Yuen
dc.contributor.authorZhang, Genen
dc.contributor.authorQi, Shu-Huaen
dc.contributor.authorQian, Pei-Yuanen
dc.date.accessioned2015-04-16T13:58:00Zen
dc.date.available2015-04-16T13:58:00Zen
dc.date.issued2012-10-24en
dc.identifier.citationEvidence for the Involvement of p38 MAPK Activation in Barnacle Larval Settlement 2012, 7 (10):e47195 PLoS ONEen
dc.identifier.issn1932-6203en
dc.identifier.pmid23115639en
dc.identifier.doi10.1371/journal.pone.0047195en
dc.identifier.urihttp://hdl.handle.net/10754/550215en
dc.description.abstractThe barnacle Balanus ( = Amphibalanus) amphitrite is a major marine fouling animal. Understanding the molecular mechanism of larval settlement in this species is critical for anti-fouling research. In this study, we cloned one isoform of p38 MAPK (Bar-p38 MAPK) from this species, which shares the significant characteristic of containing a TGY motif with other species such as yeast, Drosophila and humans. The activation of p38 MAPK was detected by an antibody that recognizes the conserved dual phosphorylation sites of TGY. The results showed that phospho-p38 MAPK (pp38 MAPK) was more highly expressed at the cyprid stage, particularly in aged cyprids, in comparison to other stages, including the nauplius and juvenile stages. Immunostaining showed that Bar-p38 MAPK and pp38 MAPK were mainly located at the cyprid antennules, and especially the third and fourth segments, which are responsible for substratum exploration during settlement. The expression and localization patterns of Bar-p38 MAPK suggest its involvement in larval settlement. This postulation was also supported by the larval settlement bioassay with the p38 MAPK inhibitor SB203580. Behavioral analysis by live imaging revealed that the larvae were still capable of exploring the surface of the substratum after SB203580 treatment. This shows that the effect of p38 MAPK on larval settlement might be by regulating the secretion of permanent proteinaceous substances. Furthermore, the level of pp38 MAPK dramatically decreased after full settlement, suggesting that Bar-p38 MAPK maybe plays a role in larval settlement rather than metamorphosis. Finally, we found that Bar-p38 MAPK was highly activated when larvae confronted extracts of adult barnacle containing settlement cues, whereas larvae pre-treated with SB203580 failed to respond to the crude adult extracts.en
dc.description.sponsorshipThis work was supported by an award from the King Abdullah University of Science and Technology (SA-C0040/UK-C0016) and grants (N-HKUST602/09 and AoE/P-04/04-II) from the Research Grants Council of the Hong Kong Special Administrative Region to PY Qian. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.relation.urlhttp://dx.plos.org/10.1371/journal.pone.0047195en
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.titleEvidence for the Involvement of p38 MAPK Activation in Barnacle Larval Settlementen
dc.typeArticleen
dc.identifier.journalPLoS ONEen
dc.identifier.pmcidPMC3480373en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDivision of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, Chinaen
dc.contributor.institutionSouth China Sea Institute of Oceanography, Chinese Academy of Science, Guangzhou, Chinaen
kaust.grant.numberSA-C0040en
kaust.grant.numberUK-C0016en
kaust.grant.programKAUST Global Collaborative Research Programen
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