Isolation of cells for selective treatment and analysis using a magnetic microfluidic chip

Handle URI:
http://hdl.handle.net/10754/550208
Title:
Isolation of cells for selective treatment and analysis using a magnetic microfluidic chip
Authors:
Yassine, Omar ( 0000-0002-0117-8017 ) ; Gooneratne, Chinthaka Pasan; Abu Smara, D.; Li, F.; Mohammed, H.; Merzaban, Jasmeen S. ( 0000-0002-7276-2907 ) ; Kosel, Jürgen ( 0000-0002-8998-8275 )
Abstract:
This study describes the development and testing of a magnetic microfluidic chip (MMC) for trapping and isolating cells tagged with superparamagnetic beads (SPBs) in a microfluidic environment for selective treatment and analysis. The trapping and isolation are done in two separate steps; first, the trapping of the tagged cells in a main channel is achieved by soft ferromagnetic disks and second, the transportation of the cells into side chambers for isolation is executed by tapered conductive paths made of Gold (Au). Numerical simulations were performed to analyze the magnetic flux and force distributions of the disks and conducting paths, for trapping and transporting SPBs. The MMC was fabricated using standard microfabrication processes. Experiments were performed with E. coli (K12 strand) tagged with 2.8 μm SPBs. The results showed that E. coli can be separated from a sample solution by trapping them at the disk sites, and then isolated into chambers by transporting them along the tapered conducting paths. Once the E. coli was trapped inside the side chambers, two selective treatments were performed. In one chamber, a solution with minimal nutrition content was added and, in another chamber, a solution with essential nutrition was added. The results showed that the growth of bacteria cultured in the second chamber containing nutrient was significantly higher, demonstrating that the E. coli was not affected by the magnetically driven transportation and the feasibility of performing different treatments on selectively isolated cells on a single microfluidic platform.
KAUST Department:
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Isolation of cells for selective treatment and analysis using a magnetic microfluidic chip 2014, 8 (3):034114 Biomicrofluidics
Publisher:
AIP Publishing
Journal:
Biomicrofluidics
Issue Date:
May-2014
DOI:
10.1063/1.4883855
PubMed ID:
25379074
PubMed Central ID:
PMC4162427
Type:
Article
ISSN:
1932-1058
Additional Links:
http://scitation.aip.org/content/aip/journal/bmf/8/3/10.1063/1.4883855
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorYassine, Omaren
dc.contributor.authorGooneratne, Chinthaka Pasanen
dc.contributor.authorAbu Smara, D.en
dc.contributor.authorLi, F.en
dc.contributor.authorMohammed, H.en
dc.contributor.authorMerzaban, Jasmeen S.en
dc.contributor.authorKosel, Jürgenen
dc.date.accessioned2015-04-16T13:45:51Zen
dc.date.available2015-04-16T13:45:51Zen
dc.date.issued2014-05en
dc.identifier.citationIsolation of cells for selective treatment and analysis using a magnetic microfluidic chip 2014, 8 (3):034114 Biomicrofluidicsen
dc.identifier.issn1932-1058en
dc.identifier.pmid25379074en
dc.identifier.doi10.1063/1.4883855en
dc.identifier.urihttp://hdl.handle.net/10754/550208en
dc.description.abstractThis study describes the development and testing of a magnetic microfluidic chip (MMC) for trapping and isolating cells tagged with superparamagnetic beads (SPBs) in a microfluidic environment for selective treatment and analysis. The trapping and isolation are done in two separate steps; first, the trapping of the tagged cells in a main channel is achieved by soft ferromagnetic disks and second, the transportation of the cells into side chambers for isolation is executed by tapered conductive paths made of Gold (Au). Numerical simulations were performed to analyze the magnetic flux and force distributions of the disks and conducting paths, for trapping and transporting SPBs. The MMC was fabricated using standard microfabrication processes. Experiments were performed with E. coli (K12 strand) tagged with 2.8 μm SPBs. The results showed that E. coli can be separated from a sample solution by trapping them at the disk sites, and then isolated into chambers by transporting them along the tapered conducting paths. Once the E. coli was trapped inside the side chambers, two selective treatments were performed. In one chamber, a solution with minimal nutrition content was added and, in another chamber, a solution with essential nutrition was added. The results showed that the growth of bacteria cultured in the second chamber containing nutrient was significantly higher, demonstrating that the E. coli was not affected by the magnetically driven transportation and the feasibility of performing different treatments on selectively isolated cells on a single microfluidic platform.en
dc.publisherAIP Publishingen
dc.relation.urlhttp://scitation.aip.org/content/aip/journal/bmf/8/3/10.1063/1.4883855en
dc.rights© 2014 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.en
dc.titleIsolation of cells for selective treatment and analysis using a magnetic microfluidic chipen
dc.typeArticleen
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalBiomicrofluidicsen
dc.identifier.pmcidPMC4162427en
dc.eprint.versionPublisher's Version/PDFen
kaust.authorYassine, Omaren
kaust.authorGooneratne, Chinthaka Pasanen
kaust.authorMerzaban, Jasmeen S.en
kaust.authorKosel, Jürgenen
kaust.authorSmara, D. Abuen
kaust.authorLi, F.en
kaust.authorMohammed, H.en

Related articles on PubMed

All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.