Soluble CD54 induces human endothelial cells ex vivo expansion useful for cardiovascular regeneration and tissue engineering application

Handle URI:
http://hdl.handle.net/10754/347300
Title:
Soluble CD54 induces human endothelial cells ex vivo expansion useful for cardiovascular regeneration and tissue engineering application
Authors:
Malara, N.M.; Trunzo, V.; Musolino, G.; Aprigliano, S.; Rotta, G.; Macrina, L.; Limongi, T.; Gratteri, S.; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 ) ; Renzulli, A.; Fini, M.; Mollace, V.
Abstract:
Aim: Consistent expansion of primary human endothelial cells in vitro is critical in the development of engineered tissue. A variety of complex culture media and techniques developed from different basal media have been reported with alternate success. Incongruous results are further confounded by donor-to-donor variability and cellular source of derivation. Our results demonstrate how to overcome these limitations using soluble CD54 (sCD54) as additive to conventional culture medium. Methods and results: Isolated primary fragment of different vessel types was expanded in Ham's F12 DMEM, enriched with growth factors, Fetal Calf Serum and conditioned medium of Human Umbilical Vein Endothelial Cells (HUVEC) collected at different passages. Cytokine content of culture media was analyzed in order to identify the soluble factors correlating with better proliferation profile. sCD54 was found to induce the in vitro expansion of human endothelial cells (HECs) independently from the vessels source and even in the absence of HUVEC-conditioned medium. The HECs cultivated in the presence of sCD54 (50 ng/ml), resulted positive for the expression of CD146 and negative for CD45, and lower fibroblast contamination. Cells were capable to proliferate with an S phase of 25%, to produce vascular endothelial growth factor, VEGF, (10 ng/ml) and to give origin to vessel-like tubule in vitro. Conclusion: Our results demonstrate that sCD54 is an essential factor for the in-vitro expansion of HECs without donor and vessel-source variability. Resulting primary cultures can be useful, for tissue engineering in regenerative medicine (e.g. artificial micro tissue generation, coating artificial heart valve etc.) and bio-nanotechnology applications. © 2015 The Authors. Published by Elsevier Ireland Ltd.
KAUST Department:
Physical Sciences and Engineering (PSE) Division
Citation:
Soluble CD54 induces human endothelial cells ex vivo expansion useful for cardiovascular regeneration and tissue engineering application 2015, 6:48 IJC Heart & Vasculature
Journal:
IJC Heart & Vasculature
Issue Date:
Mar-2015
DOI:
10.1016/j.ijcha.2015.01.004
Type:
Article
ISSN:
23529067
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S2352906715000056
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMalara, N.M.en
dc.contributor.authorTrunzo, V.en
dc.contributor.authorMusolino, G.en
dc.contributor.authorAprigliano, S.en
dc.contributor.authorRotta, G.en
dc.contributor.authorMacrina, L.en
dc.contributor.authorLimongi, T.en
dc.contributor.authorGratteri, S.en
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.contributor.authorRenzulli, A.en
dc.contributor.authorFini, M.en
dc.contributor.authorMollace, V.en
dc.date.accessioned2015-03-30T12:33:02Zen
dc.date.available2015-03-30T12:33:02Zen
dc.date.issued2015-03en
dc.identifier.citationSoluble CD54 induces human endothelial cells ex vivo expansion useful for cardiovascular regeneration and tissue engineering application 2015, 6:48 IJC Heart & Vasculatureen
dc.identifier.issn23529067en
dc.identifier.doi10.1016/j.ijcha.2015.01.004en
dc.identifier.urihttp://hdl.handle.net/10754/347300en
dc.description.abstractAim: Consistent expansion of primary human endothelial cells in vitro is critical in the development of engineered tissue. A variety of complex culture media and techniques developed from different basal media have been reported with alternate success. Incongruous results are further confounded by donor-to-donor variability and cellular source of derivation. Our results demonstrate how to overcome these limitations using soluble CD54 (sCD54) as additive to conventional culture medium. Methods and results: Isolated primary fragment of different vessel types was expanded in Ham's F12 DMEM, enriched with growth factors, Fetal Calf Serum and conditioned medium of Human Umbilical Vein Endothelial Cells (HUVEC) collected at different passages. Cytokine content of culture media was analyzed in order to identify the soluble factors correlating with better proliferation profile. sCD54 was found to induce the in vitro expansion of human endothelial cells (HECs) independently from the vessels source and even in the absence of HUVEC-conditioned medium. The HECs cultivated in the presence of sCD54 (50 ng/ml), resulted positive for the expression of CD146 and negative for CD45, and lower fibroblast contamination. Cells were capable to proliferate with an S phase of 25%, to produce vascular endothelial growth factor, VEGF, (10 ng/ml) and to give origin to vessel-like tubule in vitro. Conclusion: Our results demonstrate that sCD54 is an essential factor for the in-vitro expansion of HECs without donor and vessel-source variability. Resulting primary cultures can be useful, for tissue engineering in regenerative medicine (e.g. artificial micro tissue generation, coating artificial heart valve etc.) and bio-nanotechnology applications. © 2015 The Authors. Published by Elsevier Ireland Ltd.en
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S2352906715000056en
dc.rightsArchived with thanks to IJC Heart & Vasculature. Copyright © 2015 Published by Elsevier Ireland Ltd.en
dc.titleSoluble CD54 induces human endothelial cells ex vivo expansion useful for cardiovascular regeneration and tissue engineering applicationen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.identifier.journalIJC Heart & Vasculatureen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionInterregional Research Center for Food Safety & Health (IRC-FSH), Catanzaro, Italyen
dc.contributor.institutionBionem Laboratory, Department of Experimental Medicine, Salvatore Venuta Campus, University “Magna Graecia”, 88100 Catanzaro, Italyen
dc.contributor.institutionCellular Toxicological Laboratory, Department of Health Science, Salvatore Venuta Campus, University “Magna Graecia”, 88100 Catanzaro, Italyen
dc.contributor.institutionCardiovascular Surgery, Department of Medicine and Surgery Sciences, Salvatore Venuta Campus, University “Magna Graecia”, 88100 Catanzaro, Italyen
dc.contributor.institutionBD Biosciences Italia, Via delle Azalee 19, Buccinaso, Milan, Italyen
dc.contributor.institutionVita-Salute San Raffaele University, Milan, Italyen
dc.contributor.institutionIRCCS San Raffaele Pisana, Rome, Italyen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorLimongi, Taniaen
kaust.authorDi Fabrizio, Enzo M.en
This item is licensed under a Creative Commons License
Creative Commons
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.