Molecular-based approaches to characterize coastal microbial community and their potential relation to the trophic state of Red Sea

Handle URI:
http://hdl.handle.net/10754/346877
Title:
Molecular-based approaches to characterize coastal microbial community and their potential relation to the trophic state of Red Sea
Authors:
Ansari, Mohd Ikram ( 0000-0001-7678-4639 ) ; Harb, Moustapha; Jones, Burton ( 0000-0002-9599-1593 ) ; Hong, Pei-Ying ( 0000-0002-4474-6600 )
Abstract:
Molecular-based approaches were used to characterize the coastal microbiota and to elucidate the trophic state of Red Sea. Nutrient content and enterococci numbers were monitored, and used to correlate with the abundance of microbial markers. Microbial source tracking revealed the presence of >1 human-associated Bacteroides spp. at some of the near-shore sampling sites and at a heavily frequented beach. Water samples collected from the beaches had occasional exceedances in enterococci numbers, higher total organic carbon (TOC, 1.48-2.18 mg/L) and nitrogen (TN, 0.15-0.27 mg/L) than that detected in the near-shore waters. Enterococci abundances obtained from next-generation sequencing did not correlate well with the cultured enterococci numbers. The abundance of certain genera, for example Arcobacter, Pseudomonas and unclassified Campylobacterales, was observed to exhibit slight correlation with TOC and TN. Low abundance of functional genes accounting for up to 41 copies/L of each Pseudomonas aeruginosa and Campylobacter coli were detected. Arcobacter butzleri was also detected in abundance ranging from 111 to 238 copies/L. Operational taxonomic units (OTUs) associated with cyanobacteria, Prochlorococcus, Ostreococcus spp. and Gramella were more prevalent in waters that were likely impacted by urban runoffs and recreational activities. These OTUs could potentially serve as quantifiable markers indicative of the water quality.
KAUST Department:
Red Sea Research Center (RSRC); Water Desalination and Reuse Research Center (WDRC); Environmental Science and Engineering Program
Citation:
Molecular-based approaches to characterize coastal microbial community and their potential relation to the trophic state of Red Sea 2015, 5:9001 Scientific Reports
Publisher:
Nature Publishing Group
Journal:
Scientific Reports
Issue Date:
11-Mar-2015
DOI:
10.1038/srep09001
PubMed ID:
25758166
PubMed Central ID:
PMC4355682
Type:
Article
ISSN:
2045-2322
Additional Links:
http://www.nature.com/doifinder/10.1038/srep09001
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Environmental Science and Engineering Program; Water Desalination and Reuse Research Center (WDRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorAnsari, Mohd Ikramen
dc.contributor.authorHarb, Moustaphaen
dc.contributor.authorJones, Burtonen
dc.contributor.authorHong, Pei-Yingen
dc.date.accessioned2015-03-19T07:41:12Zen
dc.date.available2015-03-19T07:41:12Zen
dc.date.issued2015-03-11en
dc.identifier.citationMolecular-based approaches to characterize coastal microbial community and their potential relation to the trophic state of Red Sea 2015, 5:9001 Scientific Reportsen
dc.identifier.issn2045-2322en
dc.identifier.pmid25758166en
dc.identifier.doi10.1038/srep09001en
dc.identifier.urihttp://hdl.handle.net/10754/346877en
dc.description.abstractMolecular-based approaches were used to characterize the coastal microbiota and to elucidate the trophic state of Red Sea. Nutrient content and enterococci numbers were monitored, and used to correlate with the abundance of microbial markers. Microbial source tracking revealed the presence of >1 human-associated Bacteroides spp. at some of the near-shore sampling sites and at a heavily frequented beach. Water samples collected from the beaches had occasional exceedances in enterococci numbers, higher total organic carbon (TOC, 1.48-2.18 mg/L) and nitrogen (TN, 0.15-0.27 mg/L) than that detected in the near-shore waters. Enterococci abundances obtained from next-generation sequencing did not correlate well with the cultured enterococci numbers. The abundance of certain genera, for example Arcobacter, Pseudomonas and unclassified Campylobacterales, was observed to exhibit slight correlation with TOC and TN. Low abundance of functional genes accounting for up to 41 copies/L of each Pseudomonas aeruginosa and Campylobacter coli were detected. Arcobacter butzleri was also detected in abundance ranging from 111 to 238 copies/L. Operational taxonomic units (OTUs) associated with cyanobacteria, Prochlorococcus, Ostreococcus spp. and Gramella were more prevalent in waters that were likely impacted by urban runoffs and recreational activities. These OTUs could potentially serve as quantifiable markers indicative of the water quality.en
dc.publisherNature Publishing Groupen
dc.relation.urlhttp://www.nature.com/doifinder/10.1038/srep09001en
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.titleMolecular-based approaches to characterize coastal microbial community and their potential relation to the trophic state of Red Seaen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.contributor.departmentEnvironmental Science and Engineering Programen
dc.identifier.journalScientific Reportsen
dc.identifier.pmcidPMC4355682en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionWater Desalination and Reuse Center, Environmental Science and Engineeringen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorJones, Burtonen

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