Proteomic identification of early salicylate- and flg22-responsive redox-sensitive proteins in Arabidopsis

Handle URI:
http://hdl.handle.net/10754/346701
Title:
Proteomic identification of early salicylate- and flg22-responsive redox-sensitive proteins in Arabidopsis
Authors:
Liu, Pei; Zhang, Huoming ( 0000-0001-5416-0358 ) ; Yu, Boying; Xiong, Liming ( 0000-0001-8099-0806 ) ; Xia, Yiji
Abstract:
Accumulation of reactive oxygen species (ROS) is one of the early defense responses against pathogen infection in plants. The mechanism about the initial and direct regulation of the defense signaling pathway by ROS remains elusive. Perturbation of cellular redox homeostasis by ROS is believed to alter functions of redox-sensitive proteins through their oxidative modifications. Here we report an OxiTRAQ-based proteomic study in identifying proteins whose cysteines underwent oxidative modifications in Arabidopsis cells during the early response to salicylate or flg22, two defense pathway elicitors that are known to disturb cellular redox homeostasis. Among the salicylate- and/or flg22-responsive redox-sensitive proteins are those involved in transcriptional regulation, chromatin remodeling, RNA processing, post-translational modifications, and nucleocytoplasmic shuttling. The identification of the salicylate-/flg22-responsive redox-sensitive proteins provides a foundation from which further study can be conducted toward understanding biological significance of their oxidative modifications during the plant defense response.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Biosciences Core Lab
Citation:
Proteomic identification of early salicylate- and flg22-responsive redox-sensitive proteins in Arabidopsis 2015, 5:8625 Scientific Reports
Publisher:
Nature Publishing Group
Journal:
Scientific Reports
Issue Date:
27-Feb-2015
DOI:
10.1038/srep08625
PubMed ID:
25720653
PubMed Central ID:
PMC4342551
Type:
Article
ISSN:
2045-2322
Additional Links:
http://www.nature.com/doifinder/10.1038/srep08625
Appears in Collections:
Articles; Biosciences Core Lab; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLiu, Peien
dc.contributor.authorZhang, Huomingen
dc.contributor.authorYu, Boyingen
dc.contributor.authorXiong, Limingen
dc.contributor.authorXia, Yijien
dc.date.accessioned2015-03-16T05:58:03Zen
dc.date.available2015-03-16T05:58:03Zen
dc.date.issued2015-02-27en
dc.identifier.citationProteomic identification of early salicylate- and flg22-responsive redox-sensitive proteins in Arabidopsis 2015, 5:8625 Scientific Reportsen
dc.identifier.issn2045-2322en
dc.identifier.pmid25720653en
dc.identifier.doi10.1038/srep08625en
dc.identifier.urihttp://hdl.handle.net/10754/346701en
dc.description.abstractAccumulation of reactive oxygen species (ROS) is one of the early defense responses against pathogen infection in plants. The mechanism about the initial and direct regulation of the defense signaling pathway by ROS remains elusive. Perturbation of cellular redox homeostasis by ROS is believed to alter functions of redox-sensitive proteins through their oxidative modifications. Here we report an OxiTRAQ-based proteomic study in identifying proteins whose cysteines underwent oxidative modifications in Arabidopsis cells during the early response to salicylate or flg22, two defense pathway elicitors that are known to disturb cellular redox homeostasis. Among the salicylate- and/or flg22-responsive redox-sensitive proteins are those involved in transcriptional regulation, chromatin remodeling, RNA processing, post-translational modifications, and nucleocytoplasmic shuttling. The identification of the salicylate-/flg22-responsive redox-sensitive proteins provides a foundation from which further study can be conducted toward understanding biological significance of their oxidative modifications during the plant defense response.en
dc.publisherNature Publishing Groupen
dc.relation.urlhttp://www.nature.com/doifinder/10.1038/srep08625en
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.titleProteomic identification of early salicylate- and flg22-responsive redox-sensitive proteins in Arabidopsisen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBiosciences Core Laben
dc.identifier.journalScientific Reportsen
dc.identifier.pmcidPMC4342551en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Biology, Hong Kong Baptist Universityen
dc.contributor.institutionPartner State Key Laboratory of Agrobiotechnology, Chinese University of Hong Kongen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorZhang, Huomingen
kaust.authorXiong, Limingen
kaust.authorLiu, Peien
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