Development, Characterization and Cell Cultural Response of 3D Biocompatible Micro-Patterned Poly-ε-Caprolactone Scaffolds Designed and Fabricated Integrating Lithography and Micromolding Fabrication Techniques

Handle URI:
http://hdl.handle.net/10754/346685
Title:
Development, Characterization and Cell Cultural Response of 3D Biocompatible Micro-Patterned Poly-ε-Caprolactone Scaffolds Designed and Fabricated Integrating Lithography and Micromolding Fabrication Techniques
Authors:
Limongi, Tania; Miele, Ermanno; Shalabaeva, Victoria; Rocca, Rosanna La; Schipani, Rossana ( 0000-0003-3593-6522 ) ; Malara, Natalia; Angelis, Francesco de; Giugni, Andrea; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 )
Abstract:
Scaffold design and fabrication are very important subjects for biomaterial, tissue engineering and regenerative medicine research playing a unique role in tissue regeneration and repair. Among synthetic biomaterials Poly-ε- Caprolactone (PCL) is very attractive bioresorbable polyester due to its high permeability, biodegradability and capacity to be blended with other biopolymers. Thanks to its ability to naturally degrade in tissues, PCL has a great potential as a new material for implantable biomedical micro devices. This work focuses on the establishment of a micro fabrication process, by integrating lithography and micromolding fabrication techniques, for the realization of 3D microstructure PCL devices. Scaffold surface exhibits a combination in the patterned length scale; cylindrical pillars of 10 μm height and 10 μm diameter are arranged in a hexagonal lattice with periodicity of 30 μm and their sidewalls are nano-sculptured, with a regular pattern of grooves leading to a spatial modulation in the z direction. In order to demonstrate that these biocompatible pillared PCL substrates are suitable for a proper cell growth, NIH/3T3 mouse embryonic fibroblasts were seeded on them and cells key adhesion parameters were evaluated. Scanning Electron Microscopy and immunofluorescence analysis were carried out to check cell survival, proliferation and adhesion; cells growing on the PCL substrates appeared healthy and formed a well-developed network in close contact with the micro and nano features of the pillared surface. Those 3D scaffolds could be a promising solution for a wide range of applications within tissue engineering and regenerative medicine applications.
KAUST Department:
Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Development, Characterization and Cell Cultural Response of 3D Biocompatible Micro-Patterned Poly-ε-Caprolactone Scaffolds Designed and Fabricated Integrating Lithography and Micromolding Fabrication Techniques 2015, 06 (01) Journal of Tissue Science & Engineering
Publisher:
OMICS Publishing Group
Journal:
Journal of Tissue Science & Engineering
Issue Date:
12-Dec-2014
DOI:
10.4172/2157-7552.1000145
Type:
Article
ISSN:
21577552
Additional Links:
http://omicsonline.org/open-access/development-characterization-and-cell-cultural-response-of-d-biocompatible-micropatterned-polycaprolactone-scaffolds-designed-and-fabricated-integrating-lithography-2157-7552.1000145.php?aid=37109
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLimongi, Taniaen
dc.contributor.authorMiele, Ermannoen
dc.contributor.authorShalabaeva, Victoriaen
dc.contributor.authorRocca, Rosanna Laen
dc.contributor.authorSchipani, Rossanaen
dc.contributor.authorMalara, Nataliaen
dc.contributor.authorAngelis, Francesco deen
dc.contributor.authorGiugni, Andreaen
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.date.accessioned2015-03-16T05:33:07Zen
dc.date.available2015-03-16T05:33:07Zen
dc.date.issued2014-12-12en
dc.identifier.citationDevelopment, Characterization and Cell Cultural Response of 3D Biocompatible Micro-Patterned Poly-ε-Caprolactone Scaffolds Designed and Fabricated Integrating Lithography and Micromolding Fabrication Techniques 2015, 06 (01) Journal of Tissue Science & Engineeringen
dc.identifier.issn21577552en
dc.identifier.doi10.4172/2157-7552.1000145en
dc.identifier.urihttp://hdl.handle.net/10754/346685en
dc.description.abstractScaffold design and fabrication are very important subjects for biomaterial, tissue engineering and regenerative medicine research playing a unique role in tissue regeneration and repair. Among synthetic biomaterials Poly-ε- Caprolactone (PCL) is very attractive bioresorbable polyester due to its high permeability, biodegradability and capacity to be blended with other biopolymers. Thanks to its ability to naturally degrade in tissues, PCL has a great potential as a new material for implantable biomedical micro devices. This work focuses on the establishment of a micro fabrication process, by integrating lithography and micromolding fabrication techniques, for the realization of 3D microstructure PCL devices. Scaffold surface exhibits a combination in the patterned length scale; cylindrical pillars of 10 μm height and 10 μm diameter are arranged in a hexagonal lattice with periodicity of 30 μm and their sidewalls are nano-sculptured, with a regular pattern of grooves leading to a spatial modulation in the z direction. In order to demonstrate that these biocompatible pillared PCL substrates are suitable for a proper cell growth, NIH/3T3 mouse embryonic fibroblasts were seeded on them and cells key adhesion parameters were evaluated. Scanning Electron Microscopy and immunofluorescence analysis were carried out to check cell survival, proliferation and adhesion; cells growing on the PCL substrates appeared healthy and formed a well-developed network in close contact with the micro and nano features of the pillared surface. Those 3D scaffolds could be a promising solution for a wide range of applications within tissue engineering and regenerative medicine applications.en
dc.publisherOMICS Publishing Groupen
dc.relation.urlhttp://omicsonline.org/open-access/development-characterization-and-cell-cultural-response-of-d-biocompatible-micropatterned-polycaprolactone-scaffolds-designed-and-fabricated-integrating-lithography-2157-7552.1000145.php?aid=37109en
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.titleDevelopment, Characterization and Cell Cultural Response of 3D Biocompatible Micro-Patterned Poly-ε-Caprolactone Scaffolds Designed and Fabricated Integrating Lithography and Micromolding Fabrication Techniquesen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalJournal of Tissue Science & Engineeringen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionIstituto Italiano di Tecnologia (IIT), Via Morego 30, 16163, Genova, Italy.en
dc.contributor.institutionInterregional Research Center for Food Safety & Health (IRC-FSH), Department of Health Science, University "Magna Graecia" of Catanzaro, Complesso "Ninì Barbieri", 88021 Roccelletta di Borgia, Italyen
dc.contributor.institutionBIONEM, Bio-Nanotechnology and Engineering for Medicine, Department of Experimental and Clinical Medicine, University of Magna Graecia Viale Europa, Germaneto, 88100 Catanzaro, Italyen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorLimongi, Taniaen
kaust.authorSchipani, Rossanaen
kaust.authorGiugni, Andreaen
kaust.authorDi Fabrizio, Enzo M.en
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