Effects of the Diet on the Microbiota of the Red Palm Weevil (Coleoptera: Dryophthoridae)

Handle URI:
http://hdl.handle.net/10754/344290
Title:
Effects of the Diet on the Microbiota of the Red Palm Weevil (Coleoptera: Dryophthoridae)
Authors:
Montagna, Matteo; Chouaia, Bessem; Mazza, Giuseppe; Prosdocimi, Erica Maria; Crotti, Elena; Mereghetti, Valeria; Vacchini, Violetta; Giorgi, Annamaria; De Biase, Alessio; Longo, Santi; Cervo, Rita; Lozzia, Giuseppe Carlo; Alma, Alberto; Bandi, Claudio; Daffonchio, Daniele ( 0000-0003-0947-925X )
Abstract:
Rhynchophorus ferrugineus, also known as the red palm weevil, is regarded as the major pest of palm trees. Although studies of the microbiota associated with this species have been performed in recent years, little attention has been dedicated to the influence of the diet in shaping the host bacterial community. Here, we investigated the influence of food sources (i.e. palm tissues vs apple based substrate) on the microbial diversity associated with RPW, which was compared with the microbiota associated with wild individuals of the sister species Rhynchophorus vulneratus. The bacterial characterization was performed using a culture independent approach, i.e. the 16S rRNA pyrotag, and a culture dependent approach for a subset of the samples, in order to obtain bacterial isolates from RPW tissues. The bacterial community appeared significantly influenced by diet. Proteobacteria resulted to be the most abundant clade and was present in all the specimens of the three examined weevil groups. Within Proteobacteria, Enterobacteriaceae were identified in all the organs analysed, including hemolymph and reproductive organs. The apple-fed RPWs and the wild R. vulneratus showed a second dominant taxon within Firmicutes that was scarcely present in the microbiota associated with palm-fed RPWs. A comparative analysis on the bacteria associated with the palm tissues highlighted that 12 bacterial genera out of the 13 identified in the plant tissues were also present in weevils, thus indicating that palm tissues may present a source for bacterial acquisition.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Effects of the Diet on the Microbiota of the Red Palm Weevil (Coleoptera: Dryophthoridae) 2015, 10 (1):e0117439 PLOS ONE
Publisher:
Public Library of Science
Journal:
PLOS ONE
Issue Date:
30-Jan-2015
DOI:
10.1371/journal.pone.0117439
PubMed ID:
25635833
PubMed Central ID:
PMC4311986
Type:
Article
ISSN:
1932-6203
Sponsors:
This work was supported by the project BIODESERT GA-245746 “Biotechnology from Desert Microbial Extremophiles for Supporting Agriculture Research Potential in Tunisia and Southern Europe” (European Union), the Prin 2009 (grant 009L27YC8_003) from the Italian Ministry of Education, University and Research (MIUR), Linea B Piano di sviluppo Unimi 2014 (grant 15-6-3) and “Accordo di Programma, affermazione in Edolo del Centro di Eccellenza Università della Montagna” MIUR-Università degli Studi di Milano, prot. n. 1293-05/08/2011. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Additional Links:
http://dx.plos.org/10.1371/journal.pone.0117439
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMontagna, Matteoen
dc.contributor.authorChouaia, Bessemen
dc.contributor.authorMazza, Giuseppeen
dc.contributor.authorProsdocimi, Erica Mariaen
dc.contributor.authorCrotti, Elenaen
dc.contributor.authorMereghetti, Valeriaen
dc.contributor.authorVacchini, Violettaen
dc.contributor.authorGiorgi, Annamariaen
dc.contributor.authorDe Biase, Alessioen
dc.contributor.authorLongo, Santien
dc.contributor.authorCervo, Ritaen
dc.contributor.authorLozzia, Giuseppe Carloen
dc.contributor.authorAlma, Albertoen
dc.contributor.authorBandi, Claudioen
dc.contributor.authorDaffonchio, Danieleen
dc.date.accessioned2015-02-08T06:11:53Z-
dc.date.available2015-02-08T06:11:53Z-
dc.date.issued2015-01-30en
dc.identifier.citationEffects of the Diet on the Microbiota of the Red Palm Weevil (Coleoptera: Dryophthoridae) 2015, 10 (1):e0117439 PLOS ONEen
dc.identifier.issn1932-6203en
dc.identifier.pmid25635833en
dc.identifier.doi10.1371/journal.pone.0117439en
dc.identifier.urihttp://hdl.handle.net/10754/344290en
dc.description.abstractRhynchophorus ferrugineus, also known as the red palm weevil, is regarded as the major pest of palm trees. Although studies of the microbiota associated with this species have been performed in recent years, little attention has been dedicated to the influence of the diet in shaping the host bacterial community. Here, we investigated the influence of food sources (i.e. palm tissues vs apple based substrate) on the microbial diversity associated with RPW, which was compared with the microbiota associated with wild individuals of the sister species Rhynchophorus vulneratus. The bacterial characterization was performed using a culture independent approach, i.e. the 16S rRNA pyrotag, and a culture dependent approach for a subset of the samples, in order to obtain bacterial isolates from RPW tissues. The bacterial community appeared significantly influenced by diet. Proteobacteria resulted to be the most abundant clade and was present in all the specimens of the three examined weevil groups. Within Proteobacteria, Enterobacteriaceae were identified in all the organs analysed, including hemolymph and reproductive organs. The apple-fed RPWs and the wild R. vulneratus showed a second dominant taxon within Firmicutes that was scarcely present in the microbiota associated with palm-fed RPWs. A comparative analysis on the bacteria associated with the palm tissues highlighted that 12 bacterial genera out of the 13 identified in the plant tissues were also present in weevils, thus indicating that palm tissues may present a source for bacterial acquisition.en
dc.description.sponsorshipThis work was supported by the project BIODESERT GA-245746 “Biotechnology from Desert Microbial Extremophiles for Supporting Agriculture Research Potential in Tunisia and Southern Europe” (European Union), the Prin 2009 (grant 009L27YC8_003) from the Italian Ministry of Education, University and Research (MIUR), Linea B Piano di sviluppo Unimi 2014 (grant 15-6-3) and “Accordo di Programma, affermazione in Edolo del Centro di Eccellenza Università della Montagna” MIUR-Università degli Studi di Milano, prot. n. 1293-05/08/2011. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.relation.urlhttp://dx.plos.org/10.1371/journal.pone.0117439en
dc.rights© 2015 Montagna et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectWeevilsen
dc.subjectBacteriaen
dc.subjectRibosomal RNAen
dc.subjectApplesen
dc.subjectInsectsen
dc.subjectCommunity Ecologyen
dc.subjectSequence Databasesen
dc.subjectShannon indexen
dc.titleEffects of the Diet on the Microbiota of the Red Palm Weevil (Coleoptera: Dryophthoridae)en
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalPLOS ONEen
dc.identifier.pmcidPMC4311986en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDipartimento di Scienze Agrarie e Ambientali, Università degli Studi di Milano, Milano, Italyen
dc.contributor.institutionDipartimento di Scienze per gli Alimenti, la Nutrizione, l’Ambiente, Università degli Studi di Milano, Milano, Italyen
dc.contributor.institutionConsiglio per la ricerca e la sperimentazione in agricoltura, Research Centre for Agrobiology and Pedology, Cascine del Riccio, Italyen
dc.contributor.institutionDipartimento di Scienze Veterinarie e Sanità Pubblica, Università degli Studi di Milano, Milano, Italyen
dc.contributor.institutionDipartimento di Biologia e Biotecnologie “C. Darwin”, Università degli Studi di Roma “La Sapienza”, Roma, Italyen
dc.contributor.institutionDipartimento di Gestione dei Sistemi Agroalimentari e Ambientali, Università di Catania, Catania, Italyen
dc.contributor.institutionDipartimento di Biologia, Università degli Studi di Firenze, Firenze, Italyen
dc.contributor.institutionDipartimento di Scienze Agrarie, Forestali e Alimentari, Università di Torino, Grugliasco, Italyen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorDaffonchio, Danieleen

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