Lipid Droplets: A New Player in Colorectal Cancer Stem Cells Unveiled by Spectroscopic Imaging

Handle URI:
http://hdl.handle.net/10754/337898
Title:
Lipid Droplets: A New Player in Colorectal Cancer Stem Cells Unveiled by Spectroscopic Imaging
Authors:
Tirinato, Luca ( 0000-0001-9826-2129 ) ; Liberale, Carlo ( 0000-0002-5653-199X ) ; Di Franco, Simone; Candeloro, Patrizio; Benfante, Antonina; La Rocca, Rosanna; Potze, Lisette; Marotta, Roberto; Ruffilli, Roberta; Rajamanickam, Vijayakumar P.; Malerba, Mario; De Angelis, Francesco; Falqui, Andrea ( 0000-0002-1476-7742 ) ; Carbone, Ennio; Todaro, Matilde; Medema, Jan Paul; Stassi, Giorgio; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 )
Abstract:
The cancer stem cell (CSC) model is describing tumors as a hierarchical organized system and CSCs are suggested to be responsible for cancer recurrence after therapy. The identification of specific markers of CSCs is therefore of paramount importance. Here, we show that high levels of lipid droplets (LDs) are a distinctive mark of CSCs in colorectal (CR) cancer. This increased lipid content was clearly revealed by label-free Raman spectroscopy and it directly correlates with well-accepted CR-CSC markers as CD133 and Wnt pathway activity. By xenotransplantation experiments, we have finally demonstrated that CR-CSCs overexpressing LDs retain most tumorigenic potential. A relevant conceptual advance in this work is the demonstration that a cellular organelle, the LD, is a signature of CSCs, in addition to molecular markers. A further functional characterization of LDs could lead soon to design new target therapies against CR-CSCs.
KAUST Department:
Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Lipid Droplets: A New Player in Colorectal Cancer Stem Cells Unveiled by Spectroscopic Imaging 2015, 33 (1):35 STEM CELLS
Publisher:
Wiley-Blackwell
Journal:
STEM CELLS
Issue Date:
Jan-2015
DOI:
10.1002/stem.1837
PubMed ID:
25186497
PubMed Central ID:
PMC4311668
Type:
Article
ISSN:
10665099
Additional Links:
http://doi.wiley.com/10.1002/stem.1837
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorTirinato, Lucaen
dc.contributor.authorLiberale, Carloen
dc.contributor.authorDi Franco, Simoneen
dc.contributor.authorCandeloro, Patrizioen
dc.contributor.authorBenfante, Antoninaen
dc.contributor.authorLa Rocca, Rosannaen
dc.contributor.authorPotze, Lisetteen
dc.contributor.authorMarotta, Robertoen
dc.contributor.authorRuffilli, Robertaen
dc.contributor.authorRajamanickam, Vijayakumar P.en
dc.contributor.authorMalerba, Marioen
dc.contributor.authorDe Angelis, Francescoen
dc.contributor.authorFalqui, Andreaen
dc.contributor.authorCarbone, Ennioen
dc.contributor.authorTodaro, Matildeen
dc.contributor.authorMedema, Jan Paulen
dc.contributor.authorStassi, Giorgioen
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.date.accessioned2015-01-07T10:32:20Zen
dc.date.available2015-01-07T10:32:20Zen
dc.date.issued2015-01en
dc.identifier.citationLipid Droplets: A New Player in Colorectal Cancer Stem Cells Unveiled by Spectroscopic Imaging 2015, 33 (1):35 STEM CELLSen
dc.identifier.issn10665099en
dc.identifier.pmid25186497en
dc.identifier.doi10.1002/stem.1837en
dc.identifier.urihttp://hdl.handle.net/10754/337898en
dc.description.abstractThe cancer stem cell (CSC) model is describing tumors as a hierarchical organized system and CSCs are suggested to be responsible for cancer recurrence after therapy. The identification of specific markers of CSCs is therefore of paramount importance. Here, we show that high levels of lipid droplets (LDs) are a distinctive mark of CSCs in colorectal (CR) cancer. This increased lipid content was clearly revealed by label-free Raman spectroscopy and it directly correlates with well-accepted CR-CSC markers as CD133 and Wnt pathway activity. By xenotransplantation experiments, we have finally demonstrated that CR-CSCs overexpressing LDs retain most tumorigenic potential. A relevant conceptual advance in this work is the demonstration that a cellular organelle, the LD, is a signature of CSCs, in addition to molecular markers. A further functional characterization of LDs could lead soon to design new target therapies against CR-CSCs.en
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.relation.urlhttp://doi.wiley.com/10.1002/stem.1837en
dc.rightsArchived with thanks to STEM CELLSen
dc.rightsThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en
dc.titleLipid Droplets: A New Player in Colorectal Cancer Stem Cells Unveiled by Spectroscopic Imagingen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalSTEM CELLSen
dc.identifier.pmcidPMC4311668en
dc.eprint.versionPost-printen
dc.contributor.institutionCellular and Molecular Pathophysiology Laboratory, Department of Surgical and Oncological Sciences; University of Palermo; Palermo Italyen
dc.contributor.institutionBioNEM Lab, Department of Experimental and Clinical Medicine; University Magna Graecia of Catanzaro; Catanzaro Italyen
dc.contributor.institutionNanostructures and Istituto Italiano di Tecnologia; Genova Italyen
dc.contributor.institutionLaboratory for Experimental Oncology and Radiology (LEXOR); Center for Experimental Molecular Medicine (CEMM), Academic Medical Center (AMC), University of Amsterdam; Amsterdam The Netherlandsen
dc.contributor.institutionNanochemistry; Istituto Italiano di Tecnologia; Genova Italyen
dc.contributor.institutionDepartment of Microbiology Tumor and Cell Biology (MTC); Karolinska Institute; Stockholm Swedenen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorTirinato, Lucaen
kaust.authorLiberale, Carloen
kaust.authorRajamanickam, Vijayakumar Palanisamyen
kaust.authorFalqui, Andreaen
kaust.authorDi Fabrizio, Enzo M.en

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