Mechanical Stress Downregulates MHC Class I Expression on Human Cancer Cell Membrane

Handle URI:
http://hdl.handle.net/10754/337778
Title:
Mechanical Stress Downregulates MHC Class I Expression on Human Cancer Cell Membrane
Authors:
La Rocca, Rosanna; Tallerico, Rossana; Talib Hassan, Almosawy; Das, Gobind ( 0000-0003-0942-681X ) ; Tadepally, Lakshmikanth; Matteucci, Marco; Liberale, Carlo ( 0000-0002-5653-199X ) ; Mesuraca, Maria; Scumaci, Domenica; Gentile, Francesco; Cojoc, Gheorghe; Perozziello, Gerardo; Ammendolia, Antonio; Gallo, Adriana; Kärre, Klas; Cuda, Giovanni; Candeloro, Patrizio; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 ) ; Carbone, Ennio
Abstract:
In our body, cells are continuously exposed to physical forces that can regulate different cell functions such as cell proliferation, differentiation and death. In this work, we employed two different strategies to mechanically stress cancer cells. The cancer and healthy cell populations were treated either with mechanical stress delivered by a micropump (fabricated by deep X-ray nanolithography) or by ultrasound wave stimuli. A specific down-regulation of Major Histocompatibility Complex (MHC) class I molecules expression on cancer cell membrane compared to different kinds of healthy cells (fibroblasts, macrophages, dendritic and lymphocyte cells) was observed, stimulating the cells with forces in the range of nano-newton, and pressures between 1 and 10 bar (1 bar = 100.000 Pascal), depending on the devices used. Moreover, Raman spectroscopy analysis, after mechanical treatment, in the range between 700–1800 cm−1, indicated a relative concentration variation of MHC class I. PCA analysis was also performed to distinguish control and stressed cells within different cell lines. These mechanical induced phenotypic changes increase the tumor immunogenicity, as revealed by the related increased susceptibility to Natural Killer (NK) cells cytotoxic recognition.
KAUST Department:
Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
La Rocca R, Tallerico R, Talib Hassan A, Das G, Tadepally L, et al. (2014) Mechanical Stress Downregulates MHC Class I Expression on Human Cancer Cell Membrane. PLoS ONE 9(12): e111758. doi:10.1371/journal.pone.0111758
Publisher:
Public Library of Science
Journal:
PLoS ONE
Issue Date:
26-Dec-2014
DOI:
10.1371/journal.pone.0111758
PubMed ID:
25541692
PubMed Central ID:
PMC4277281
Type:
Article
ISSN:
1932-6203
Sponsors:
Ennio Carbone's work has been supported by a UICC International Cancer Technology Transfer Fellowship, grant AIRC-IG 10189, and grant AIRC 15521. Rossana Tallerico is a Post Doc awarded by triennial fellowships “Luciana Selce” FIRC. Giovanni Cuda has been supported by PON01_02834 Prometeo (Ministry of Education and Research) and PONa3_00435 Biomedpark@UMG (Ministry of Education and Research). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Additional Links:
http://dx.plos.org/10.1371/journal.pone.0111758
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLa Rocca, Rosannaen
dc.contributor.authorTallerico, Rossanaen
dc.contributor.authorTalib Hassan, Almosawyen
dc.contributor.authorDas, Gobinden
dc.contributor.authorTadepally, Lakshmikanthen
dc.contributor.authorMatteucci, Marcoen
dc.contributor.authorLiberale, Carloen
dc.contributor.authorMesuraca, Mariaen
dc.contributor.authorScumaci, Domenicaen
dc.contributor.authorGentile, Francescoen
dc.contributor.authorCojoc, Gheorgheen
dc.contributor.authorPerozziello, Gerardoen
dc.contributor.authorAmmendolia, Antonioen
dc.contributor.authorGallo, Adrianaen
dc.contributor.authorKärre, Klasen
dc.contributor.authorCuda, Giovannien
dc.contributor.authorCandeloro, Patrizioen
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.contributor.authorCarbone, Ennioen
dc.date.accessioned2015-01-04T13:48:11Z-
dc.date.available2015-01-04T13:48:11Z-
dc.date.issued2014-12-26en
dc.identifier.citationLa Rocca R, Tallerico R, Talib Hassan A, Das G, Tadepally L, et al. (2014) Mechanical Stress Downregulates MHC Class I Expression on Human Cancer Cell Membrane. PLoS ONE 9(12): e111758. doi:10.1371/journal.pone.0111758en
dc.identifier.issn1932-6203en
dc.identifier.pmid25541692en
dc.identifier.doi10.1371/journal.pone.0111758en
dc.identifier.urihttp://hdl.handle.net/10754/337778en
dc.description.abstractIn our body, cells are continuously exposed to physical forces that can regulate different cell functions such as cell proliferation, differentiation and death. In this work, we employed two different strategies to mechanically stress cancer cells. The cancer and healthy cell populations were treated either with mechanical stress delivered by a micropump (fabricated by deep X-ray nanolithography) or by ultrasound wave stimuli. A specific down-regulation of Major Histocompatibility Complex (MHC) class I molecules expression on cancer cell membrane compared to different kinds of healthy cells (fibroblasts, macrophages, dendritic and lymphocyte cells) was observed, stimulating the cells with forces in the range of nano-newton, and pressures between 1 and 10 bar (1 bar = 100.000 Pascal), depending on the devices used. Moreover, Raman spectroscopy analysis, after mechanical treatment, in the range between 700–1800 cm−1, indicated a relative concentration variation of MHC class I. PCA analysis was also performed to distinguish control and stressed cells within different cell lines. These mechanical induced phenotypic changes increase the tumor immunogenicity, as revealed by the related increased susceptibility to Natural Killer (NK) cells cytotoxic recognition.en
dc.description.sponsorshipEnnio Carbone's work has been supported by a UICC International Cancer Technology Transfer Fellowship, grant AIRC-IG 10189, and grant AIRC 15521. Rossana Tallerico is a Post Doc awarded by triennial fellowships “Luciana Selce” FIRC. Giovanni Cuda has been supported by PON01_02834 Prometeo (Ministry of Education and Research) and PONa3_00435 Biomedpark@UMG (Ministry of Education and Research). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.relation.urlhttp://dx.plos.org/10.1371/journal.pone.0111758en
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectCancer treatmenten
dc.subjectCell membranesen
dc.subjectLymphocytesen
dc.subjectMHC class I genesen
dc.subjectMajor histocompatibility complexen
dc.subjectMechanical stressen
dc.subjectMembrane proteinsen
dc.subjectNK cellsen
dc.titleMechanical Stress Downregulates MHC Class I Expression on Human Cancer Cell Membraneen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalPLoS ONEen
dc.identifier.pmcidPMC4277281en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Experimental and Clinical Medicine, University of “Magna Graecia”, Catanzaro, Italyen
dc.contributor.institutionItalian Institute of Technology (IIT), Genova, Italyen
dc.contributor.institutionDepartment of Microbiology, College of Medicine, University of Thi-Qar, Nasseriah, Iraqen
dc.contributor.institutionScience for Life Laboratory, Department of Medicine, Karolinska Institute, Stockholm, Swedenen
dc.contributor.institutionNanotech Department of Micro- and Nanotechnology, Technical University of Denmark, Kongens Lyngby, Denmarken
dc.contributor.institutionDepartment of Surgical and Medical Sciences, University of “Magna Graecia”, Catanzaro, Italyen
dc.contributor.institutionEndocrinology and Experimental Oncology Institute, CNR, Napoli, Italyen
dc.contributor.institutionDepartment of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Swedenen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorDas, Gobinden
kaust.authorLiberale, Carloen
kaust.authorDi Fabrizio, Enzo M.en

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