Stable Composition of the Nano- and Picoplankton Community during the Ocean Iron Fertilization Experiment LOHAFEX

Handle URI:
http://hdl.handle.net/10754/336938
Title:
Stable Composition of the Nano- and Picoplankton Community during the Ocean Iron Fertilization Experiment LOHAFEX
Authors:
Thiele, Stefan; Wolf, Christian; Schulz, Isabelle Katharina; Assmy, Philipp; Metfies, Katja; Fuchs, Bernhard M.
Abstract:
The iron fertilization experiment LOHAFEX was conducted in a cold-core eddy in the Southern Atlantic Ocean during austral summer. Within a few days after fertilization, a phytoplankton bloom developed dominated by nano- and picoplankton groups. Unlike previously reported for other iron fertilization experiments, a diatom bloom was prevented by iron and silicate co-limitation. We used 18S rRNA gene tag pyrosequencing to investigate the diversity of these morphologically similar cell types within the nano- and picoplankton and microscopically enumerated dominant clades after catalyzed reported deposition fluorescence in situ hybridization (CARD-FISH) with specific oligonucleotide probes. In addition to Phaeocystis, members of Syndiniales group II, clade 10–11, and the Micromonas clades ABC and E made up a major fraction of the tag sequences of the nano- and picoplankton community within the fertilized patch. However, the same clades were also dominant before the bloom and outside the fertilized patch. Furthermore, only little changes in diversity could be observed over the course of the experiment. These results were corroborated by CARD-FISH analysis which confirmed the presence of a stable nano- and picoplankton community dominated by Phaeocystis and Micromonas during the entire course of the experiment. Interestingly, although Syndiniales dominated the tag sequences, they could hardly be detected by CARD-FISH, possibly due to the intracellular parasitic life style of this clade. The remarkable stability of the nano- and picoplankton community points to a tight coupling of the different trophic levels within the microbial food web during LOHAFEX.
KAUST Department:
Red Sea Research Center (RSRC)
Citation:
Thiele S, Wolf C, Schulz IK, Assmy P, Metfies K, et al. (2014) Stable Composition of the Nano- and Picoplankton Community during the Ocean Iron Fertilization Experiment LOHAFEX. PLoS ONE 9(11): e113244. doi:10.1371/journal.pone.0113244
Publisher:
PLOS
Journal:
PLoS ONE
Issue Date:
17-Nov-2014
DOI:
10.1371/journal.pone.0113244
PubMed ID:
25401706
PubMed Central ID:
PMC4234645
Type:
Article
ISSN:
1932-6203
Sponsors:
This work was funded by the Max Planck Society and the Helmholtz Association (Young Investigator Group PLANKTOSENS, VH-NG-500).
Additional Links:
http://dx.plos.org/10.1371/journal.pone.0113244
Appears in Collections:
Articles; Red Sea Research Center (RSRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorThiele, Stefanen
dc.contributor.authorWolf, Christianen
dc.contributor.authorSchulz, Isabelle Katharinaen
dc.contributor.authorAssmy, Philippen
dc.contributor.authorMetfies, Katjaen
dc.contributor.authorFuchs, Bernhard M.en
dc.date.accessioned2014-12-08T15:31:17Z-
dc.date.available2014-12-08T15:31:17Z-
dc.date.issued2014-11-17en
dc.identifier.citationThiele S, Wolf C, Schulz IK, Assmy P, Metfies K, et al. (2014) Stable Composition of the Nano- and Picoplankton Community during the Ocean Iron Fertilization Experiment LOHAFEX. PLoS ONE 9(11): e113244. doi:10.1371/journal.pone.0113244en
dc.identifier.issn1932-6203en
dc.identifier.pmid25401706en
dc.identifier.doi10.1371/journal.pone.0113244en
dc.identifier.urihttp://hdl.handle.net/10754/336938en
dc.description.abstractThe iron fertilization experiment LOHAFEX was conducted in a cold-core eddy in the Southern Atlantic Ocean during austral summer. Within a few days after fertilization, a phytoplankton bloom developed dominated by nano- and picoplankton groups. Unlike previously reported for other iron fertilization experiments, a diatom bloom was prevented by iron and silicate co-limitation. We used 18S rRNA gene tag pyrosequencing to investigate the diversity of these morphologically similar cell types within the nano- and picoplankton and microscopically enumerated dominant clades after catalyzed reported deposition fluorescence in situ hybridization (CARD-FISH) with specific oligonucleotide probes. In addition to Phaeocystis, members of Syndiniales group II, clade 10–11, and the Micromonas clades ABC and E made up a major fraction of the tag sequences of the nano- and picoplankton community within the fertilized patch. However, the same clades were also dominant before the bloom and outside the fertilized patch. Furthermore, only little changes in diversity could be observed over the course of the experiment. These results were corroborated by CARD-FISH analysis which confirmed the presence of a stable nano- and picoplankton community dominated by Phaeocystis and Micromonas during the entire course of the experiment. Interestingly, although Syndiniales dominated the tag sequences, they could hardly be detected by CARD-FISH, possibly due to the intracellular parasitic life style of this clade. The remarkable stability of the nano- and picoplankton community points to a tight coupling of the different trophic levels within the microbial food web during LOHAFEX.en
dc.description.sponsorshipThis work was funded by the Max Planck Society and the Helmholtz Association (Young Investigator Group PLANKTOSENS, VH-NG-500).en
dc.language.isoenen
dc.publisherPLOSen
dc.relation.urlhttp://dx.plos.org/10.1371/journal.pone.0113244en
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectDiatomsen
dc.subjectFertilizationen
dc.subjectLight microscopyen
dc.subjectPhylogenetic analysisen
dc.subjectPhytoplanktonen
dc.subjectRibosomal RNAen
dc.subjectSequence Databasesen
dc.subjectSequence tagged site analysisen
dc.titleStable Composition of the Nano- and Picoplankton Community during the Ocean Iron Fertilization Experiment LOHAFEXen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalPLoS ONEen
dc.identifier.pmcidPMC4234645en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Molecular Ecology, Max Planck Institute for Marine Microbiology, Bremen, Germanyen
dc.contributor.institutionDepartment of Polar Biological Oceanography, Division of Bioscience, Alfred Wegener Institute - Helmholtz Centre for Polar and Marine Research, Bremerhaven, Germanyen
dc.contributor.institutionBremen International Graduate School for Marine Sciences (GLOMAR), MARUM - Center for Marine Environmental Sciences, University of Bremen, Bremen, Germanyen
dc.contributor.institutionCenter for Ice, Climate and Ecosystems (ICE), Norwegian Polar Institute, Fram Centre, Tromsø, Norwayen
dc.contributor.institutionStazione Zoologica Anton Dohrn, Naples, Italyen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorSchulz, Isabelle Katharinaen

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