Oligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeast

Handle URI:
http://hdl.handle.net/10754/334973
Title:
Oligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeast
Authors:
Tehseen, Muhammad ( 0000-0001-9834-2372 ) ; Liao, Chunyan; Dacres, Helen; Dumancic, Mira; Trowell, Stephen; Anderson, Alisha
Abstract:
It is widely accepted that vertebrate G-Protein Coupled Receptors (GPCRs) associate with each other as homo- or hetero-dimers or higher-order oligomers. The C. elegans genome encodes hundreds of olfactory GPCRs, which may be expressed in fewer than a dozen chemosensory neurons, suggesting an opportunity for oligomerisation. Here we show, using three independent lines of evidence: co-immunoprecipitation, bioluminescence resonance energy transfer and a yeast two-hybrid assay that nematode olfactory receptors (ORs) oligomerise when heterologously expressed in yeast. Specifically, the nematode receptor ODR-10 is able to homo-oligomerise and can also form heteromers with the related nematode receptor STR-112. ODR-10 also oligomerised with the rat I7 OR but did not oligomerise with the human somatostatin receptor 5, a neuropeptide receptor. In this study, the question of functional relevance was not addressed and remains to be investigated.
KAUST Department:
Desert Agriculture Initiative; Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Oligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeast 2014, 9 (9):e108680 PLoS ONE
Publisher:
Public Library of Science (PLoS)
Journal:
PLoS ONE
Issue Date:
25-Sep-2014
DOI:
10.1371/journal.pone.0108680
PubMed ID:
25254556
PubMed Central ID:
PMC4177895
Type:
Article
ISSN:
1932-6203
Sponsors:
The research is funded by CSIRO. However, the funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Additional Links:
http://dx.plos.org/10.1371/journal.pone.0108680
Appears in Collections:
Articles; Desert Agriculture Initiative; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorTehseen, Muhammaden
dc.contributor.authorLiao, Chunyanen
dc.contributor.authorDacres, Helenen
dc.contributor.authorDumancic, Miraen
dc.contributor.authorTrowell, Stephenen
dc.contributor.authorAnderson, Alishaen
dc.date.accessioned2014-11-16T11:38:57Z-
dc.date.available2014-11-16T11:38:57Z-
dc.date.issued2014-09-25en
dc.identifier.citationOligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeast 2014, 9 (9):e108680 PLoS ONEen
dc.identifier.issn1932-6203en
dc.identifier.pmid25254556en
dc.identifier.doi10.1371/journal.pone.0108680en
dc.identifier.urihttp://hdl.handle.net/10754/334973en
dc.description.abstractIt is widely accepted that vertebrate G-Protein Coupled Receptors (GPCRs) associate with each other as homo- or hetero-dimers or higher-order oligomers. The C. elegans genome encodes hundreds of olfactory GPCRs, which may be expressed in fewer than a dozen chemosensory neurons, suggesting an opportunity for oligomerisation. Here we show, using three independent lines of evidence: co-immunoprecipitation, bioluminescence resonance energy transfer and a yeast two-hybrid assay that nematode olfactory receptors (ORs) oligomerise when heterologously expressed in yeast. Specifically, the nematode receptor ODR-10 is able to homo-oligomerise and can also form heteromers with the related nematode receptor STR-112. ODR-10 also oligomerised with the rat I7 OR but did not oligomerise with the human somatostatin receptor 5, a neuropeptide receptor. In this study, the question of functional relevance was not addressed and remains to be investigated.en
dc.description.sponsorshipThe research is funded by CSIRO. However, the funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.language.isoenen
dc.publisherPublic Library of Science (PLoS)en
dc.relation.urlhttp://dx.plos.org/10.1371/journal.pone.0108680en
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectcell fusionen
dc.subjectcell membranesen
dc.subjectco-immunoprecipitationen
dc.subjectnematodaen
dc.subjectOlfactory receptor neuronsen
dc.subjectpolymerase chain reactionen
dc.subjectTranscription Factorsen
dc.subjectYeast two-hybrid assaysen
dc.titleOligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeasten
dc.typeArticleen
dc.contributor.departmentDesert Agriculture Initiativeen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalPLoS ONEen
dc.identifier.pmcidPMC4177895en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionCSIRO Food Futures National Research Flagship & CSIRO Ecosystem Sciences, Australia, Canberra, ACT, Australiaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorTehseen, Muhammaden

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