An Optimized Table-Top Small-Angle X-ray Scattering Set-up for the Nanoscale Structural Analysis of Soft Matter

Handle URI:
http://hdl.handle.net/10754/334951
Title:
An Optimized Table-Top Small-Angle X-ray Scattering Set-up for the Nanoscale Structural Analysis of Soft Matter
Authors:
Sibillano, T.; De Caro, L.; Altamura, D.; Siliqi, D.; Ramella, M.; Boccafoschi, F.; Ciasca, G.; Campi, G.; Tirinato, Luca ( 0000-0001-9826-2129 ) ; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 ) ; Giannini, C.
Abstract:
The paper shows how a table top superbright microfocus laboratory X-ray source and an innovative restoring-data algorithm, used in combination, allow to analyze the super molecular structure of soft matter by means of Small Angle X-ray Scattering ex-situ experiments. The proposed theoretical approach is aimed to restore diffraction features from SAXS profiles collected from low scattering biomaterials or soft tissues, and therefore to deal with extremely noisy diffraction SAXS profiles/maps. As biological test cases we inspected: i) residues of exosomes' drops from healthy epithelial colon cell line and colorectal cancer cells; ii) collagen/human elastin artificial scaffolds developed for vascular tissue engineering applications; iii) apoferritin protein in solution. Our results show how this combination can provide morphological/structural nanoscale information to characterize new artificial biomaterials and/or to get insight into the transition between healthy and pathological tissues during the progression of a disease, or to morphologically characterize nanoscale proteins, based on SAXS data collected in a room-sized laboratory.
KAUST Department:
Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Sibillano, T., De Caro, L., Altamura, D., Siliqi, D., Ramella, M., Boccafoschi, F., . . . Giannini, C. (2014). An Optimized Table-Top Small-Angle X-ray Scattering Set-up for the Nanoscale Structural Analysis of Soft Matter. Sci. Rep., 4. doi: 10.1038/srep06985
Publisher:
Nature Publishing Group
Journal:
Scientific Reports
Issue Date:
10-Nov-2014
DOI:
10.1038/srep06985
Type:
Article
ISSN:
2045-2322
Additional Links:
http://www.nature.com/doifinder/10.1038/srep06985
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorSibillano, T.en
dc.contributor.authorDe Caro, L.en
dc.contributor.authorAltamura, D.en
dc.contributor.authorSiliqi, D.en
dc.contributor.authorRamella, M.en
dc.contributor.authorBoccafoschi, F.en
dc.contributor.authorCiasca, G.en
dc.contributor.authorCampi, G.en
dc.contributor.authorTirinato, Lucaen
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.contributor.authorGiannini, C.en
dc.date.accessioned2014-11-16T07:24:22Z-
dc.date.available2014-11-16T07:24:22Z-
dc.date.issued2014-11-10en
dc.identifier.citationSibillano, T., De Caro, L., Altamura, D., Siliqi, D., Ramella, M., Boccafoschi, F., . . . Giannini, C. (2014). An Optimized Table-Top Small-Angle X-ray Scattering Set-up for the Nanoscale Structural Analysis of Soft Matter. Sci. Rep., 4. doi: 10.1038/srep06985en
dc.identifier.issn2045-2322en
dc.identifier.doi10.1038/srep06985en
dc.identifier.urihttp://hdl.handle.net/10754/334951en
dc.description.abstractThe paper shows how a table top superbright microfocus laboratory X-ray source and an innovative restoring-data algorithm, used in combination, allow to analyze the super molecular structure of soft matter by means of Small Angle X-ray Scattering ex-situ experiments. The proposed theoretical approach is aimed to restore diffraction features from SAXS profiles collected from low scattering biomaterials or soft tissues, and therefore to deal with extremely noisy diffraction SAXS profiles/maps. As biological test cases we inspected: i) residues of exosomes' drops from healthy epithelial colon cell line and colorectal cancer cells; ii) collagen/human elastin artificial scaffolds developed for vascular tissue engineering applications; iii) apoferritin protein in solution. Our results show how this combination can provide morphological/structural nanoscale information to characterize new artificial biomaterials and/or to get insight into the transition between healthy and pathological tissues during the progression of a disease, or to morphologically characterize nanoscale proteins, based on SAXS data collected in a room-sized laboratory.en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.relation.urlhttp://www.nature.com/doifinder/10.1038/srep06985en
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectCharacterization and analytical techniquesen
dc.subjectX-raysen
dc.titleAn Optimized Table-Top Small-Angle X-ray Scattering Set-up for the Nanoscale Structural Analysis of Soft Matteren
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalScientific Reportsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionIstituto di Cristallografia (IC-CNR), via Amendola 122/O, I-70126 Bari, Italyen
dc.contributor.institutionDepartment of Health Sciences, University of Piemonte Orientale “A. Avogadro”, 28100 Novara, Italyen
dc.contributor.institutionIstituto di Fisica, Universitá Cattolica S. Cuore, L.go Francesco Vito 1 I-00168, Roma, Italyen
dc.contributor.institutionIstituto di Cristallografia (IC-CNR), Via Salaria Km 29.300, 00015 Monterotondo, Roma, Italyen
dc.contributor.institutionBIONEMlab University Magna Graecia, Department of Clinical and Experimental Medicine, Viale Europa, 88100 Catanzaro, Italyen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorDi Fabrizio, Enzo M.en
kaust.authorDi Fabrizio, Enzo M.en
kaust.authorTirinato, Lucaen
kaust.authorTirinato, Lucaen
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