Characterization of new microsatellite loci for population genetic studies in the Smooth Cauliflower Coral (Stylophora sp.)

Handle URI:
http://hdl.handle.net/10754/334649
Title:
Characterization of new microsatellite loci for population genetic studies in the Smooth Cauliflower Coral (Stylophora sp.)
Authors:
Banguera-Hinestroza, E.; Saenz-Agudelo, P.; Bayer, T.; Berumen, Michael L. ( 0000-0003-2463-2742 ) ; Voolstra, Christian R. ( 0000-0003-4555-3795 )
Abstract:
A total of one hundred microsatellites loci were selected from the draft genome of Stylophora pistillata and evaluated in previously characterized samples of Stylophora cf pistillata from the Red Sea. 17 loci were amplified successfully and tested in 24 individuals from samples belonging to a single population from the central region of the Red Sea. The number of alleles ranged from 3 to 15 alleles per locus, while observed heterozygosity ranged from 0. 292 to 0. 95. Six of these loci showed significant deviations from Hardy-Weinberg equilibrium (HWE) expectations, and 4/136 paired loci comparisons suggested linkage disequilibrium after Bonferroni corrections. After excluding loci with significant HWE deviation and evidence of null alleles, average genetic diversity over loci in the population studied (N = 24, Nloci = 11) was 0. 701 ± 0. 380. This indicates that these loci can be used effectively to evaluate genetic diversity and undertake population genetics studies in Stylophora sp. populations. 2013 The Author(s).
KAUST Department:
Red Sea Research Center (RSRC)
Citation:
Banguera-Hinestroza E, Saenz-Agudelo P, Bayer T, Berumen ML, Voolstra CR (2013) Characterization of new microsatellite loci for population genetic studies in the Smooth Cauliflower Coral (Stylophora sp.). Conservation Genet Resour 5: 561-563. doi:10.1007/s12686-012-9852-x.
Publisher:
Springer Nature
Journal:
Conservation Genetics Resources
Issue Date:
9-Jan-2013
DOI:
10.1007/s12686-012-9852-x
Type:
Article
ISSN:
18777252
Appears in Collections:
Articles; Red Sea Research Center (RSRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorBanguera-Hinestroza, E.en
dc.contributor.authorSaenz-Agudelo, P.en
dc.contributor.authorBayer, T.en
dc.contributor.authorBerumen, Michael L.en
dc.contributor.authorVoolstra, Christian R.en
dc.date.accessioned2014-11-11T14:33:36Z-
dc.date.available2014-11-11T14:33:36Z-
dc.date.issued2013-01-09en
dc.identifier.citationBanguera-Hinestroza E, Saenz-Agudelo P, Bayer T, Berumen ML, Voolstra CR (2013) Characterization of new microsatellite loci for population genetic studies in the Smooth Cauliflower Coral (Stylophora sp.). Conservation Genet Resour 5: 561-563. doi:10.1007/s12686-012-9852-x.en
dc.identifier.issn18777252en
dc.identifier.doi10.1007/s12686-012-9852-xen
dc.identifier.urihttp://hdl.handle.net/10754/334649en
dc.description.abstractA total of one hundred microsatellites loci were selected from the draft genome of Stylophora pistillata and evaluated in previously characterized samples of Stylophora cf pistillata from the Red Sea. 17 loci were amplified successfully and tested in 24 individuals from samples belonging to a single population from the central region of the Red Sea. The number of alleles ranged from 3 to 15 alleles per locus, while observed heterozygosity ranged from 0. 292 to 0. 95. Six of these loci showed significant deviations from Hardy-Weinberg equilibrium (HWE) expectations, and 4/136 paired loci comparisons suggested linkage disequilibrium after Bonferroni corrections. After excluding loci with significant HWE deviation and evidence of null alleles, average genetic diversity over loci in the population studied (N = 24, Nloci = 11) was 0. 701 ± 0. 380. This indicates that these loci can be used effectively to evaluate genetic diversity and undertake population genetics studies in Stylophora sp. populations. 2013 The Author(s).en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.en
dc.rightsArchived with thanks to Conservation Genetics Resourcesen
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/en
dc.subjectCoralen
dc.subjectRed Seaen
dc.subjectStylophoraen
dc.subjectTetra and dinucleotide microsatellitesen
dc.subjectAnthozoaen
dc.subjectBrassica oleracea var. botrytisen
dc.subjectStylophora (coral)en
dc.subjectStylophora pistillataen
dc.subjectTetraen
dc.titleCharacterization of new microsatellite loci for population genetic studies in the Smooth Cauliflower Coral (Stylophora sp.)en
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalConservation Genetics Resourcesen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionBiology Department, Woods Hole Oceanographic Institution, Woods Hole, MA, 02543, United Statesen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorSaenz Agudelo, Pabloen
kaust.authorBayer, Tillen
kaust.authorBerumen, Michael L.en
kaust.authorVoolstra, Christian R.en
kaust.authorBanguera Hinestroza, Eulaliaen
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