Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID

Handle URI:
http://hdl.handle.net/10754/334618
Title:
Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID
Authors:
Zourelidou, Melina; Absmanner, Birgit; Weller, Benjamin; Barbosa, Inês CR; Willige, Björn C; Fastner, Astrid; Streit, Verena; Port, Sarah A; Colcombet, Jean; de la Fuente van Bentem, Sergio; Hirt, Heribert ( 0000-0003-3119-9633 ) ; Kuster, Bernhard; Schulze, Waltraud X; Hammes, Ulrich Z; Schwechheimer, Claus
Abstract:
The development and morphology of vascular plants is critically determined by synthesis and proper distribution of the phytohormone auxin. The directed cell-to-cell distribution of auxin is achieved through a system of auxin influx and efflux transporters. PIN-FORMED (PIN) proteins are proposed auxin efflux transporters, and auxin fluxes can seemingly be predicted based on the-in many cells-asymmetric plasma membrane distribution of PINs. Here, we show in a heterologous Xenopus oocyte system as well as in Arabidopsis thaliana inflorescence stems that PIN-mediated auxin transport is directly activated by D6 PROTEIN KINASE (D6PK) and PINOID (PID)/WAG kinases of the Arabidopsis AGCVIII kinase family. At the same time, we reveal that D6PKs and PID have differential phosphosite preferences. Our study suggests that PIN activation by protein kinases is a crucial component of auxin transport control that must be taken into account to understand auxin distribution within the plant.
KAUST Department:
Center for Desert Agriculture
Citation:
Zourelidou M, Absmanner B, Weller B, Barbosa IC, Willige BC, et al. (2014) Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID. eLife 3. doi:10.7554/eLife.02860.
Publisher:
eLife Sciences Publications, Ltd
Journal:
eLife
Issue Date:
19-Jun-2014
DOI:
10.7554/eLife.02860
PubMed ID:
24948515
PubMed Central ID:
PMC4091124
Type:
Article
ISSN:
2050084X
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorZourelidou, Melinaen
dc.contributor.authorAbsmanner, Birgiten
dc.contributor.authorWeller, Benjaminen
dc.contributor.authorBarbosa, Inês CRen
dc.contributor.authorWillige, Björn Cen
dc.contributor.authorFastner, Astriden
dc.contributor.authorStreit, Verenaen
dc.contributor.authorPort, Sarah Aen
dc.contributor.authorColcombet, Jeanen
dc.contributor.authorde la Fuente van Bentem, Sergioen
dc.contributor.authorHirt, Heriberten
dc.contributor.authorKuster, Bernharden
dc.contributor.authorSchulze, Waltraud Xen
dc.contributor.authorHammes, Ulrich Zen
dc.contributor.authorSchwechheimer, Clausen
dc.date.accessioned2014-11-11T14:32:17Zen
dc.date.available2014-11-11T14:32:17Zen
dc.date.issued2014-06-19en
dc.identifier.citationZourelidou M, Absmanner B, Weller B, Barbosa IC, Willige BC, et al. (2014) Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID. eLife 3. doi:10.7554/eLife.02860.en
dc.identifier.issn2050084Xen
dc.identifier.pmid24948515en
dc.identifier.doi10.7554/eLife.02860en
dc.identifier.urihttp://hdl.handle.net/10754/334618en
dc.description.abstractThe development and morphology of vascular plants is critically determined by synthesis and proper distribution of the phytohormone auxin. The directed cell-to-cell distribution of auxin is achieved through a system of auxin influx and efflux transporters. PIN-FORMED (PIN) proteins are proposed auxin efflux transporters, and auxin fluxes can seemingly be predicted based on the-in many cells-asymmetric plasma membrane distribution of PINs. Here, we show in a heterologous Xenopus oocyte system as well as in Arabidopsis thaliana inflorescence stems that PIN-mediated auxin transport is directly activated by D6 PROTEIN KINASE (D6PK) and PINOID (PID)/WAG kinases of the Arabidopsis AGCVIII kinase family. At the same time, we reveal that D6PKs and PID have differential phosphosite preferences. Our study suggests that PIN activation by protein kinases is a crucial component of auxin transport control that must be taken into account to understand auxin distribution within the plant.en
dc.language.isoenen
dc.publishereLife Sciences Publications, Ltden
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.en
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/en
dc.titleAuxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOIDen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journaleLifeen
dc.identifier.pmcidPMC4091124en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Plant Systems Biology, Technische Universitt Mnchen, Freising, Germanyen
dc.contributor.institutionDepartment of Cell Biology and Plant Biochemistry, Universitt Regensburg, Regensburg, Germanyen
dc.contributor.institutionUnit de Recherche en Gnomique Vgtale, Universit Evry, Evry, Franceen
dc.contributor.institutionDepartment of Plant Molecular Biology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austriaen
dc.contributor.institutionProteomics and Bioanalytics, Technische Universitt Mnchen, Freising, Germanyen
dc.contributor.institutionMax-Planck-Institute of Molecular Plant Physiology, Potsdam, Germanyen
dc.contributor.institutionPlant Systems Biology, Universitt Hohenheim, Hohenheim, Germanyen
dc.contributor.institutionSalk Institute for Biological Studies, La Jolla, United Statesen
dc.contributor.institutionDepartment of Molecular Biology, Gttingen University Medical Center, Gttingen, Germanyen
dc.contributor.institutionSyngenta Seeds B.V, Enkhuizen, Netherlandsen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorHirt, Heriberten

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.