Antimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureus

Handle URI:
http://hdl.handle.net/10754/334610
Title:
Antimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureus
Authors:
Huang, Jonathan P.; Mojib, Nazia ( 0000-0003-4924-5538 ) ; Goli, Rakesh R.; Watkins, Samantha; Waites, Ken B.; Ravindra, Rasik; Andersen, Dale T.; Bej, Asim K.
Abstract:
Multiple drug resistant (MDR) and methicillin-resistant Staphylococcus aureus (MRSA) have become increasingly prevalent as a community acquired infection. As a result limited treatment options are available with conventional synthetic antibiotics. Bioprospecting natural products with potent antimicrobial activity show promise for developing new drugs against this pathogen. In this study, we have investigated the antimicrobial activity of a purple violet pigment (PVP) from an Antarctic bacterium, Janthinobacterium sp. Ant5-2 on 15 clinical MDR and MRSA strains. The colorimetric resazurin assay was employed to determine the minimum inhibitory concentration (MIC90) of PVP against MDR and MRSA. The MIC90 ranged between 1.57 µg/mL and 3.13 µg/mL, which are significantly lower than many antimicrobials tested from natural sources against this pathogen. The spectrophotometrically determined growth analysis and total microscopic counts using Live/dead® BacLight™ fluorescent stain exhibited a steady decrease in viability of both MDR and MRSA cultures following treatment with PVP at the MIC levels. In silico predictive molecular docking study revealed that PVP could be a DNA-targeting minor groove binding antimicrobial compound. The continued development of novel antimicrobials derived from natural sources with the combination of a suite of conventional antibiotics could stem the rising pandemic of MDR and MRSA along with other deadly microbial pathogens.
KAUST Department:
Red Sea Research Center (RSRC)
Citation:
Huang JP, Mojib N, Goli RR, Watkins S, Waites KB, et al. (2012) Antimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureus. Natural Products and Bioprospecting 2: 104-110. doi:10.1007/s13659-012-0021-4.
Publisher:
Springer Science + Business Media
Journal:
Natural Products and Bioprospecting
Issue Date:
11-Apr-2012
DOI:
10.1007/s13659-012-0021-4
PubMed Central ID:
PMC4131597
Type:
Article
ISSN:
2192-2195
Appears in Collections:
Articles; Red Sea Research Center (RSRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorHuang, Jonathan P.en
dc.contributor.authorMojib, Naziaen
dc.contributor.authorGoli, Rakesh R.en
dc.contributor.authorWatkins, Samanthaen
dc.contributor.authorWaites, Ken B.en
dc.contributor.authorRavindra, Rasiken
dc.contributor.authorAndersen, Dale T.en
dc.contributor.authorBej, Asim K.en
dc.date.accessioned2014-11-11T14:31:55Z-
dc.date.available2014-11-11T14:31:55Z-
dc.date.issued2012-04-11en
dc.identifier.citationHuang JP, Mojib N, Goli RR, Watkins S, Waites KB, et al. (2012) Antimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureus. Natural Products and Bioprospecting 2: 104-110. doi:10.1007/s13659-012-0021-4.en
dc.identifier.issn2192-2195en
dc.identifier.doi10.1007/s13659-012-0021-4en
dc.identifier.urihttp://hdl.handle.net/10754/334610en
dc.description.abstractMultiple drug resistant (MDR) and methicillin-resistant Staphylococcus aureus (MRSA) have become increasingly prevalent as a community acquired infection. As a result limited treatment options are available with conventional synthetic antibiotics. Bioprospecting natural products with potent antimicrobial activity show promise for developing new drugs against this pathogen. In this study, we have investigated the antimicrobial activity of a purple violet pigment (PVP) from an Antarctic bacterium, Janthinobacterium sp. Ant5-2 on 15 clinical MDR and MRSA strains. The colorimetric resazurin assay was employed to determine the minimum inhibitory concentration (MIC90) of PVP against MDR and MRSA. The MIC90 ranged between 1.57 µg/mL and 3.13 µg/mL, which are significantly lower than many antimicrobials tested from natural sources against this pathogen. The spectrophotometrically determined growth analysis and total microscopic counts using Live/dead® BacLight™ fluorescent stain exhibited a steady decrease in viability of both MDR and MRSA cultures following treatment with PVP at the MIC levels. In silico predictive molecular docking study revealed that PVP could be a DNA-targeting minor groove binding antimicrobial compound. The continued development of novel antimicrobials derived from natural sources with the combination of a suite of conventional antibiotics could stem the rising pandemic of MDR and MRSA along with other deadly microbial pathogens.en
dc.language.isoenen
dc.publisherSpringer Science + Business Mediaen
dc.rightsArchived with thanks to Natural Products and Bioprospectingen
dc.titleAntimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureusen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalNatural Products and Bioprospectingen
dc.identifier.pmcidPMC4131597en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Biology, University of Alabama at Birmingham, Birmingham, AL 35294-1170 USAen
dc.contributor.institutionDepartment of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294-1170 USAen
dc.contributor.institutionHead Land Sada, National Centre for Antarctic & Ocean Research, Vasco-da-Gama Goa, 403804 Indiaen
dc.contributor.institutionCarl Sagan Center for the Study of Life in the Universe, SETI Institute, Mountain View, CA 94043 USAen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorMojib, Naziaen
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.