A Biochemical/Biophysical Assay Dyad for HTS-Compatible Triaging of Inhibitors of the HIV-1 Nef/Hck SH3 Interaction

Handle URI:
http://hdl.handle.net/10754/334597
Title:
A Biochemical/Biophysical Assay Dyad for HTS-Compatible Triaging of Inhibitors of the HIV-1 Nef/Hck SH3 Interaction
Authors:
Breuer, Sebastian; Espinola, Sheryll; Morelli, Xavier; Torbett, Bruce E; Arold, Stefan T. ( 0000-0001-5278-0668 ) ; Engels, Ingo H
Abstract:
The current treatment regimens for HIV include over 20 anti-retrovirals. However, adverse drug effects and the emergence of drug resistance necessitates the continued improvement of the existing drug classes as well as the development of novel drugs that target as yet therapeutically unexploited viral and cellular pathways. Here we demonstrate a strategy for the discovery of protein-protein interaction inhibitors of the viral pathogenicity factor HIV-1 Nef and its interaction with the host factor SH3. A combination of a time-resolved fluorescence resonance energy resonance energy transfer-based assay and a label-free resonant waveguide grating-based assay was optimized for high-throughput screening formats.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Computational Bioscience Research Center (CBRC)
Citation:
Breuer S (2013) A Biochemical/Biophysical Assay Dyad for HTS-Compatible Triaging of Inhibitors of the HIV-1 Nef/Hck SH3 Interaction. Current Chemical Genomics and Translational Medicine 7: 16-20. doi:10.2174/2213988501307010016.
Publisher:
Bentham Open
Journal:
Current Chemical Genomics and Translational Medicine
Issue Date:
26-Jul-2013
DOI:
10.2174/2213988501307010016
PubMed ID:
24396731
PubMed Central ID:
PMC3854662
Type:
Article
ISSN:
2213-9885
Appears in Collections:
Articles; Computational Bioscience Research Center (CBRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorBreuer, Sebastianen
dc.contributor.authorEspinola, Sheryllen
dc.contributor.authorMorelli, Xavieren
dc.contributor.authorTorbett, Bruce Een
dc.contributor.authorArold, Stefan T.en
dc.contributor.authorEngels, Ingo Hen
dc.date.accessioned2014-11-11T14:31:26Z-
dc.date.available2014-11-11T14:31:26Z-
dc.date.issued2013-07-26en
dc.identifier.citationBreuer S (2013) A Biochemical/Biophysical Assay Dyad for HTS-Compatible Triaging of Inhibitors of the HIV-1 Nef/Hck SH3 Interaction. Current Chemical Genomics and Translational Medicine 7: 16-20. doi:10.2174/2213988501307010016.en
dc.identifier.issn2213-9885en
dc.identifier.pmid24396731en
dc.identifier.doi10.2174/2213988501307010016en
dc.identifier.urihttp://hdl.handle.net/10754/334597en
dc.description.abstractThe current treatment regimens for HIV include over 20 anti-retrovirals. However, adverse drug effects and the emergence of drug resistance necessitates the continued improvement of the existing drug classes as well as the development of novel drugs that target as yet therapeutically unexploited viral and cellular pathways. Here we demonstrate a strategy for the discovery of protein-protein interaction inhibitors of the viral pathogenicity factor HIV-1 Nef and its interaction with the host factor SH3. A combination of a time-resolved fluorescence resonance energy resonance energy transfer-based assay and a label-free resonant waveguide grating-based assay was optimized for high-throughput screening formats.en
dc.language.isoenen
dc.publisherBentham Openen
dc.rightsThis is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.en
dc.rightsArchived with thanks to Current Chemical Genomics and Translational Medicineen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/en
dc.titleA Biochemical/Biophysical Assay Dyad for HTS-Compatible Triaging of Inhibitors of the HIV-1 Nef/Hck SH3 Interactionen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journalCurrent Chemical Genomics and Translational Medicineen
dc.identifier.pmcidPMC3854662en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USAen
dc.contributor.institutionGenomics Institute of the Novartis Research Foundation, 10675 John Jay Hopkins Dr, San Diego, CA 92121, USAen
dc.contributor.institutionCRCM, CNRS UMR7258, Laboratory of Integrative Structural and Chemical Biology (ISCB); INSERM, U1068; Institut Paoli‐Calmettes; Aix‐Marseille Université, UM105, F‐13009, Marseille, Franceen
dc.contributor.institutionDepartment of Biochemistry and Molecular Biology, Unit 1000, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USAen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorArold, Stefan T.en

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.