MKK3 Was Involved in Larval Settlement of the Barnacle Amphibalanus amphitrite through Activating the Kinase Activity of p38MAPK

Handle URI:
http://hdl.handle.net/10754/334592
Title:
MKK3 Was Involved in Larval Settlement of the Barnacle Amphibalanus amphitrite through Activating the Kinase Activity of p38MAPK
Authors:
Zhang, Gen; He, Li-Sheng; Wong, Yue Him; Qian, Pei-Yuan
Abstract:
The p38 mitogen-activated protein kinase (p38MAPK) plays a key role in larval settlement of the barnacle Amphibalanus amphitrite. To study the signaling pathway associated with p38MAPK during larval settlement, we sought to identify the upstream kinase of p38MAPK. Three MKKs (MKK3, MKK4 and MKK7) and three MAPKs (p38MAPK, ERK and JNK) in A. amphitrite were cloned and recombinantly expressed in E. coli. Through kinase assays, we found that MKK3, but not MKK4 or MKK7, phosphorylated p38MAPK. Furthermore, MKK3 activity was specific to p38MAPK, as it did not phosphorylate ERK or JNK. To further investigate the functional relationship between MKK3 and p38MAPK in vivo, we studied the localization of phospho-MKK3 (pMKK3) and MKK3 by immunostaining. Consistent with the patterns of p38MAPK and phospho-p38MAPK (pp38MAPK), pMKK3 and MKK3 mainly localized to the antennules of the cyprids. Western blot analysis revealed that pMKK3 levels, like pp38MAPK levels, were elevated at cyprid stage, compared to nauplii and juvenile stages. Moreover, pMKK3 levels increased after treatment with adult barnacle crude extracts, suggesting that MKK3 might mediate the stimulatory effects of adult barnacle extracts on the p38MAPK pathway. © 2013 Zhang et al.
Citation:
Zhang G, He L-S, Wong YH, Qian P-Y (2013) MKK3 Was Involved in Larval Settlement of the Barnacle Amphibalanus amphitrite through Activating the Kinase Activity of p38MAPK. PLoS ONE 8: e69510. doi:10.1371/journal.pone.0069510.
Publisher:
Public Library of Science (PLoS)
Journal:
PLoS ONE
KAUST Grant Number:
SA-C0040; UK-C0016
Issue Date:
29-Jul-2013
DOI:
10.1371/journal.pone.0069510
PubMed ID:
23922727
PubMed Central ID:
PMC3726695
Type:
Article
ISSN:
1932-6203
Sponsors:
This work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association (http://www.comra.org), an award from the King Abdullah University of Science and Technology (SA-C0040/UK-C0016; http://www.kaust.edu.sa) and grants (GRF661611, GRF662413 and AoE/P-04/04-II) from the Research Grants Council (http://www.ugc.edu.hk/eng/rgc/index.htm) of the Hong Kong Special Administrative Region. The funders did not contribute to the experimental design, operation or manuscript writing. Additionally, the funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorZhang, Genen
dc.contributor.authorHe, Li-Shengen
dc.contributor.authorWong, Yue Himen
dc.contributor.authorQian, Pei-Yuanen
dc.date.accessioned2014-11-11T14:31:13Z-
dc.date.available2014-11-11T14:31:13Z-
dc.date.issued2013-07-29en
dc.identifier.citationZhang G, He L-S, Wong YH, Qian P-Y (2013) MKK3 Was Involved in Larval Settlement of the Barnacle Amphibalanus amphitrite through Activating the Kinase Activity of p38MAPK. PLoS ONE 8: e69510. doi:10.1371/journal.pone.0069510.en
dc.identifier.issn1932-6203en
dc.identifier.pmid23922727en
dc.identifier.doi10.1371/journal.pone.0069510en
dc.identifier.urihttp://hdl.handle.net/10754/334592en
dc.description.abstractThe p38 mitogen-activated protein kinase (p38MAPK) plays a key role in larval settlement of the barnacle Amphibalanus amphitrite. To study the signaling pathway associated with p38MAPK during larval settlement, we sought to identify the upstream kinase of p38MAPK. Three MKKs (MKK3, MKK4 and MKK7) and three MAPKs (p38MAPK, ERK and JNK) in A. amphitrite were cloned and recombinantly expressed in E. coli. Through kinase assays, we found that MKK3, but not MKK4 or MKK7, phosphorylated p38MAPK. Furthermore, MKK3 activity was specific to p38MAPK, as it did not phosphorylate ERK or JNK. To further investigate the functional relationship between MKK3 and p38MAPK in vivo, we studied the localization of phospho-MKK3 (pMKK3) and MKK3 by immunostaining. Consistent with the patterns of p38MAPK and phospho-p38MAPK (pp38MAPK), pMKK3 and MKK3 mainly localized to the antennules of the cyprids. Western blot analysis revealed that pMKK3 levels, like pp38MAPK levels, were elevated at cyprid stage, compared to nauplii and juvenile stages. Moreover, pMKK3 levels increased after treatment with adult barnacle crude extracts, suggesting that MKK3 might mediate the stimulatory effects of adult barnacle extracts on the p38MAPK pathway. © 2013 Zhang et al.en
dc.description.sponsorshipThis work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association (http://www.comra.org), an award from the King Abdullah University of Science and Technology (SA-C0040/UK-C0016; http://www.kaust.edu.sa) and grants (GRF661611, GRF662413 and AoE/P-04/04-II) from the Research Grants Council (http://www.ugc.edu.hk/eng/rgc/index.htm) of the Hong Kong Special Administrative Region. The funders did not contribute to the experimental design, operation or manuscript writing. Additionally, the funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.language.isoenen
dc.publisherPublic Library of Science (PLoS)en
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectmitogen activated protein kinase kinase 3en
dc.subjectmitogen activated protein kinase kinase 4en
dc.subjectmitogen activated protein kinase kinase 7en
dc.subjectmitogen activated protein kinase p38en
dc.subjectstress activated protein kinaseen
dc.subjectAmphibalanus amphitriteen
dc.subjectbarnacleen
dc.subjectcypris (larva)en
dc.subjectenzyme activityen
dc.subjectimmunohistochemistryen
dc.subjectin vivo studyen
dc.subjectlarval developmenten
dc.subjectnauplius (larva)en
dc.subjectnucleotide sequenceen
dc.subjectprotein localizationen
dc.subjectprotein phosphorylationen
dc.subjectWestern blottingen
dc.subjectExtracellular Signal-Regulated MAP Kinasesen
dc.subjectJNK Mitogen-Activated Protein Kinasesen
dc.subjectLarvaen
dc.subjectMAP Kinase Kinase 3en
dc.subjectMAP Kinase Kinase 4en
dc.subjectMAP Kinase Kinase 7en
dc.subjectp38 Mitogen-Activated Protein Kinasesen
dc.subjectThoracicaen
dc.titleMKK3 Was Involved in Larval Settlement of the Barnacle Amphibalanus amphitrite through Activating the Kinase Activity of p38MAPKen
dc.typeArticleen
dc.identifier.journalPLoS ONEen
dc.identifier.pmcidPMC3726695en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionKAUST Global Collaborative Research Program, Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, Chinaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorZhang, Genen
kaust.authorHe, Li-Shengen
kaust.authorWong, Yue Himen
kaust.authorQian, Pei-Yuanen
kaust.grant.numberSA-C0040en
kaust.grant.numberUK-C0016en
kaust.grant.programKAUST Global Collaborative Research Programen

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