Sea urchin akt activity is Runx-dependent and required for post-cleavage stage cell division

Handle URI:
http://hdl.handle.net/10754/334589
Title:
Sea urchin akt activity is Runx-dependent and required for post-cleavage stage cell division
Authors:
Robertson, Anthony J.; Coluccio, Alison; Jensen, Sarah; Rydlizky, Katarina; Coffman, James A.
Abstract:
In animal development following the initial cleavage stage of embryogenesis, the cell cycle becomes dependent on intercellular signaling and controlled by the genomically encoded ontogenetic program. Runx transcription factors are critical regulators of metazoan developmental signaling, and we have shown that the sea urchin Runx gene runt-1, which is globally expressed during early embryogenesis, functions in support of blastula stage cell proliferation and expression of the mitogenic genes pkc1, cyclinD, and several wnts. To obtain a more comprehensive list of early runt-1 regulatory targets, we screened a Strongylocentrotus purpuratus microarray to identify genes mis-expressed in mid-blastula stage runt-1 morphants. This analysis showed that loss of Runx function perturbs the expression of multiple genes involved in cell division, including the pro-growth and survival kinase Akt (PKB), which is significantly underexpressed in runt-1 morphants. Further genomic analysis revealed that Akt is encoded by two genes in the S. purpuratus genome, akt-1 and akt-2, both of which contain numerous canonical Runx target sequences. The transcripts of both genes accumulate several fold during blastula stage, contingent on runt-1 expression. Inhibiting Akt expression or activity causes blastula stage cell cycle arrest, whereas overexpression of akt-1 mRNA rescues cell proliferation in runt-1 morphants. These results indicate that post-cleavage stage cell division requires Runx-dependent expression of akt.
KAUST Department:
Present address: King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia
Citation:
Robertson AJ, Coluccio A, Jensen S, Rydlizky K, Coffman JA (2013) Sea urchin akt activity is Runx-dependent and required for post-cleavage stage cell division. Biology Open 2: 472-478. doi:10.1242/bio.20133913.
Publisher:
The Company of Biologists
Journal:
Biology Open
Issue Date:
25-Mar-2013
DOI:
10.1242/bio.20133913
PubMed ID:
23789095
PubMed Central ID:
PMC3654265
Type:
Article
ISSN:
2046-6390
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorRobertson, Anthony J.en
dc.contributor.authorColuccio, Alisonen
dc.contributor.authorJensen, Sarahen
dc.contributor.authorRydlizky, Katarinaen
dc.contributor.authorCoffman, James A.en
dc.date.accessioned2014-11-11T14:31:05Z-
dc.date.available2014-11-11T14:31:05Z-
dc.date.issued2013-03-25en
dc.identifier.citationRobertson AJ, Coluccio A, Jensen S, Rydlizky K, Coffman JA (2013) Sea urchin akt activity is Runx-dependent and required for post-cleavage stage cell division. Biology Open 2: 472-478. doi:10.1242/bio.20133913.en
dc.identifier.issn2046-6390en
dc.identifier.pmid23789095en
dc.identifier.doi10.1242/bio.20133913en
dc.identifier.urihttp://hdl.handle.net/10754/334589en
dc.description.abstractIn animal development following the initial cleavage stage of embryogenesis, the cell cycle becomes dependent on intercellular signaling and controlled by the genomically encoded ontogenetic program. Runx transcription factors are critical regulators of metazoan developmental signaling, and we have shown that the sea urchin Runx gene runt-1, which is globally expressed during early embryogenesis, functions in support of blastula stage cell proliferation and expression of the mitogenic genes pkc1, cyclinD, and several wnts. To obtain a more comprehensive list of early runt-1 regulatory targets, we screened a Strongylocentrotus purpuratus microarray to identify genes mis-expressed in mid-blastula stage runt-1 morphants. This analysis showed that loss of Runx function perturbs the expression of multiple genes involved in cell division, including the pro-growth and survival kinase Akt (PKB), which is significantly underexpressed in runt-1 morphants. Further genomic analysis revealed that Akt is encoded by two genes in the S. purpuratus genome, akt-1 and akt-2, both of which contain numerous canonical Runx target sequences. The transcripts of both genes accumulate several fold during blastula stage, contingent on runt-1 expression. Inhibiting Akt expression or activity causes blastula stage cell cycle arrest, whereas overexpression of akt-1 mRNA rescues cell proliferation in runt-1 morphants. These results indicate that post-cleavage stage cell division requires Runx-dependent expression of akt.en
dc.language.isoenen
dc.publisherThe Company of Biologistsen
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0/).en
dc.rightsArchived with thanks to Biology Openen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en
dc.titleSea urchin akt activity is Runx-dependent and required for post-cleavage stage cell divisionen
dc.typeArticleen
dc.contributor.departmentPresent address: King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabiaen
dc.identifier.journalBiology Openen
dc.identifier.pmcidPMC3654265en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionOld Bar Harbor Road, Salisbury Cove, ME 04672en
dc.contributor.institutionPresent address: Boyce Thompson Institute for Plant Research, Ithaca, New York, USAen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorRobertson, Anthony J.en

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