Differential expression of proteins and phosphoproteins during larval metamorphosis of the polychaete Capitella sp. I

Handle URI:
http://hdl.handle.net/10754/334584
Title:
Differential expression of proteins and phosphoproteins during larval metamorphosis of the polychaete Capitella sp. I
Authors:
Chandramouli, Kondethimmanahalli ( 0000-0002-0104-1993 ) ; Soo, Lisa; Qian, Pei-Yuan
Abstract:
Background: The spontaneous metamorphosis of the polychaete Capitella sp. I larvae into juveniles requires minor morphological changes, including segment formation, body elongation, and loss of cilia. In this study, we investigated changes in the expression patterns of both proteins and phosphoproteins during the transition from larvae to juveniles in this species. We used two-dimensional gel electrophoresis (2-DE) followed by multiplex fluorescent staining and MALDI-TOF mass spectrometry analysis to identify the differentially expressed proteins as well as the protein and phosphoprotein profiles of both competent larvae and juveniles.Results: Twenty-three differentially expressed proteins were identified in the two developmental stages. Expression patterns of two of those proteins were examined at the protein level by Western blot analysis while seven were further studied at the mRNA level by real-time PCR. Results showed that proteins related to cell division, cell migration, energy storage and oxidative stress were plentifully expressed in the competent larvae; in contrast, proteins involved in oxidative metabolism and transcriptional regulation were abundantly expressed in the juveniles.Conclusion: It is likely that these differentially expressed proteins are involved in regulating the larval metamorphosis process and can be used as protein markers for studying molecular mechanisms associated with larval metamorphosis in polychaetes. © 2011 Chandramouli et al; licensee BioMed Central Ltd.
Citation:
Chandramouli KH, Soo L, Qian P-Y (2011) Differential expression of proteins and phosphoproteins during larval metamorphosis of the polychaete Capitella sp. I. Proteome Science 9: 51. doi:10.1186/1477-5956-9-51.
Publisher:
Springer Nature
Journal:
Proteome Science
KAUST Grant Number:
SA-C0040; UK-C0016
Issue Date:
3-Sep-2011
DOI:
10.1186/1477-5956-9-51
PubMed ID:
21888661
PubMed Central ID:
PMC3180302
Type:
Article
ISSN:
1477-5956
Sponsors:
The authors thank Mr. Y Zhang for his technical help in generating the 2-DE gels and Mr. Yue Him Wong for help with RT-PCR. We are also thankful to Dr. On On Lee for critically reviewing the manuscript and Cherry Kwan for proof-reading the manuscript. This study was supported by an award from the King Abdullah University of Science and Technology (SA-C0040/UK-C0016) and a grant from the Research Grants Council of the Hong Kong Special Administrative Region (AoE/P-04/04-II) to P.-Y. Qian.
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Full metadata record

DC FieldValue Language
dc.contributor.authorChandramouli, Kondethimmanahallien
dc.contributor.authorSoo, Lisaen
dc.contributor.authorQian, Pei-Yuanen
dc.date.accessioned2014-11-11T14:30:47Z-
dc.date.available2014-11-11T14:30:47Z-
dc.date.issued2011-09-03en
dc.identifier.citationChandramouli KH, Soo L, Qian P-Y (2011) Differential expression of proteins and phosphoproteins during larval metamorphosis of the polychaete Capitella sp. I. Proteome Science 9: 51. doi:10.1186/1477-5956-9-51.en
dc.identifier.issn1477-5956en
dc.identifier.pmid21888661en
dc.identifier.doi10.1186/1477-5956-9-51en
dc.identifier.urihttp://hdl.handle.net/10754/334584en
dc.description.abstractBackground: The spontaneous metamorphosis of the polychaete Capitella sp. I larvae into juveniles requires minor morphological changes, including segment formation, body elongation, and loss of cilia. In this study, we investigated changes in the expression patterns of both proteins and phosphoproteins during the transition from larvae to juveniles in this species. We used two-dimensional gel electrophoresis (2-DE) followed by multiplex fluorescent staining and MALDI-TOF mass spectrometry analysis to identify the differentially expressed proteins as well as the protein and phosphoprotein profiles of both competent larvae and juveniles.Results: Twenty-three differentially expressed proteins were identified in the two developmental stages. Expression patterns of two of those proteins were examined at the protein level by Western blot analysis while seven were further studied at the mRNA level by real-time PCR. Results showed that proteins related to cell division, cell migration, energy storage and oxidative stress were plentifully expressed in the competent larvae; in contrast, proteins involved in oxidative metabolism and transcriptional regulation were abundantly expressed in the juveniles.Conclusion: It is likely that these differentially expressed proteins are involved in regulating the larval metamorphosis process and can be used as protein markers for studying molecular mechanisms associated with larval metamorphosis in polychaetes. © 2011 Chandramouli et al; licensee BioMed Central Ltd.en
dc.description.sponsorshipThe authors thank Mr. Y Zhang for his technical help in generating the 2-DE gels and Mr. Yue Him Wong for help with RT-PCR. We are also thankful to Dr. On On Lee for critically reviewing the manuscript and Cherry Kwan for proof-reading the manuscript. This study was supported by an award from the King Abdullah University of Science and Technology (SA-C0040/UK-C0016) and a grant from the Research Grants Council of the Hong Kong Special Administrative Region (AoE/P-04/04-II) to P.-Y. Qian.en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/en
dc.subject2-DEphosen
dc.subjectCapitella sp. Ien
dc.subjectLarval metamorphosisen
dc.subjectMultiplexed proteomicsen
dc.subjectPhoproteomeen
dc.subjectRT-PCRen
dc.subjectactinen
dc.subjectenolase phosphataseen
dc.subjectheat shock protein 90en
dc.subjectisocitrate dehydrogenaseen
dc.subjectisocitrate dehydrogenase isoenzymeen
dc.subjectmessenger RNAen
dc.subjectphosphataseen
dc.subjectphosphoproteinen
dc.subjectphosphoproteomeen
dc.subjectproteinen
dc.subjectproteomeen
dc.subjectproton transporting adenosine triphosphate synthaseen
dc.subjectthioredoxin peroxidaseen
dc.subjecttropomyosinen
dc.subjecttubulinen
dc.subjecttyrosine 3 monooxygenaseen
dc.subjectunclassified drugen
dc.subjectvitellogeninen
dc.subjectaerobic metabolismen
dc.subjectCapitellaen
dc.subjectcell divisionen
dc.subjectcell migrationen
dc.subjectdown regulationen
dc.subjectgel electrophoresisen
dc.subjectgene expressionen
dc.subjectlarval stageen
dc.subjectmatrix assisted laser desorption ionization time of flight mass spectrometryen
dc.subjectmetamorphosisen
dc.subjectoxidative stressen
dc.subjectprotein analysisen
dc.subjectprotein expressionen
dc.subjectprotein metabolismen
dc.subjectprotein phosphorylationen
dc.subjectproteomicsen
dc.subjectreal time polymerase chain reactionen
dc.subjecttranscription regulationen
dc.subjectupregulationen
dc.subjectWestern blottingen
dc.subjectwormen
dc.subjectCapitellaen
dc.subjectPolychaetaen
dc.titleDifferential expression of proteins and phosphoproteins during larval metamorphosis of the polychaete Capitella sp. Ien
dc.typeArticleen
dc.identifier.journalProteome Scienceen
dc.identifier.pmcidPMC3180302en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionKAUST Global Collaborative Research, Division of Life Science, Hong Kong University of Science and Technology, Hong Kong SAR, Chinaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorChandramouli, Kondethimmanahallien
kaust.authorSoo, Lisaen
kaust.authorQian, Pei-Yuanen
kaust.grant.numberSA-C0040en
kaust.grant.numberUK-C0016en
kaust.grant.programKAUST Global Collaborative Research Programen
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