Phosphoproteome analysis during larval development and metamorphosis in the spionid polychaete Pseudopolydora vexillosa

Handle URI:
http://hdl.handle.net/10754/334583
Title:
Phosphoproteome analysis during larval development and metamorphosis in the spionid polychaete Pseudopolydora vexillosa
Authors:
Chandramouli, Kondethimmanahalli ( 0000-0002-0104-1993 ) ; Mok, Flora SY; Wang, Hao; Qian, Pei-Yuan
Abstract:
Background: The metamorphosis of the spionid polychaete Pseudopolydora vexillosa includes spontaneous settlement onto soft-bottom habitats and morphogenesis that can be completed in a very short time. A previous study on the total changes to the proteome during the various developmental stages of P. vexillosa suggested that little or no de novo protein synthesis occurs during metamorphosis. In this study, we used multicolor fluorescence detection of proteins in 2-D gels for differential analysis of proteins and phosphoproteins to reveal the dynamics of post-translational modification proteins in this species. A combination of affinity chromatography, 2D-PAGE, and mass spectrometry was used to identify the phosphoproteins in pre-competent larvae, competent larvae, and newly metamorphosed juveniles. Results: We reproducibly detected 210, 492, and 172 phosphoproteins in pre-competent larvae, competent larvae, and newly metamorphosed juveniles, respectively. The highest percentage of phosphorylation was observed during the competent larval stage. About 64 stage-specific phosphoprotein spots were detected in the competent stage, and 32 phosphoproteins were found to be significantly differentially expressed in the three stages. We identified 38 phosphoproteins, 10 of which were differentially expressed during metamorphosis. These phosphoproteins belonged to six categories of biological processes: (1) development, (2) cell differentiation and integrity, (3) transcription and translation, (4) metabolism, (5) protein-protein interaction and proteolysis, and (6) receptors and enzymes. Conclusion: This is the first study to report changes in phosphoprotein expression patterns during the metamorphosis of the marine polychaete P. vexillosa. The higher degree of phosphorylation during the process of attaining competence to settle and metamorphose may be due to fast morphological transitions regulated by various mechanisms. Our data are consistent with previous studies showing a high percentage of phosphorylation during competency in the barnacle Balanus amphitrite and the bryozoan Bugula neritina. The identified phosphoproteins may play an important role during metamorphosis, and further studies on the location and functions of important proteins during metamorphosis are warranted. © 2011 Chandramouli et al; licensee BioMed Central Ltd.
Citation:
Chandramouli KH, Mok FS, Wang H, Qian P-Y (2011) Phosphoproteome analysis during larval development and metamorphosis in the spionid polychaete Pseudopolydora vexillosa. BMC Developmental Biology 11: 31. doi:10.1186/1471-213X-11-31.
Publisher:
Springer Nature
Journal:
BMC Developmental Biology
KAUST Grant Number:
SA-C0040; UK-C0016
Issue Date:
25-May-2011
DOI:
10.1186/1471-213X-11-31
PubMed ID:
21612608
PubMed Central ID:
PMC3115903
Type:
Article
ISSN:
1471-213X
Sponsors:
The authors thank Mr. Y. Zhang for his technical help in generating the 2-DE gels, and Dr. S. Arellano for critically reviewing the manuscript and Cherry Kwan for proof-reading the manuscript. This study was supported by an award from King Abdullah University of Science and Technology (SA-C0040/UK-C0016), a grant from the China Ocean Mineral Resource Research and Development Association (COMARRD06/07.Sc02), and grants from the Research Grants Council of the Hong Kong Special Administrative Region (N-HKUST602/09, 662408 and AoE/P-04/04-II) to P.-Y. Qian.
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Publications Acknowledging KAUST Support

Full metadata record

DC FieldValue Language
dc.contributor.authorChandramouli, Kondethimmanahallien
dc.contributor.authorMok, Flora SYen
dc.contributor.authorWang, Haoen
dc.contributor.authorQian, Pei-Yuanen
dc.date.accessioned2014-11-11T14:30:44Z-
dc.date.available2014-11-11T14:30:44Z-
dc.date.issued2011-05-25en
dc.identifier.citationChandramouli KH, Mok FS, Wang H, Qian P-Y (2011) Phosphoproteome analysis during larval development and metamorphosis in the spionid polychaete Pseudopolydora vexillosa. BMC Developmental Biology 11: 31. doi:10.1186/1471-213X-11-31.en
dc.identifier.issn1471-213Xen
dc.identifier.pmid21612608en
dc.identifier.doi10.1186/1471-213X-11-31en
dc.identifier.urihttp://hdl.handle.net/10754/334583en
dc.description.abstractBackground: The metamorphosis of the spionid polychaete Pseudopolydora vexillosa includes spontaneous settlement onto soft-bottom habitats and morphogenesis that can be completed in a very short time. A previous study on the total changes to the proteome during the various developmental stages of P. vexillosa suggested that little or no de novo protein synthesis occurs during metamorphosis. In this study, we used multicolor fluorescence detection of proteins in 2-D gels for differential analysis of proteins and phosphoproteins to reveal the dynamics of post-translational modification proteins in this species. A combination of affinity chromatography, 2D-PAGE, and mass spectrometry was used to identify the phosphoproteins in pre-competent larvae, competent larvae, and newly metamorphosed juveniles. Results: We reproducibly detected 210, 492, and 172 phosphoproteins in pre-competent larvae, competent larvae, and newly metamorphosed juveniles, respectively. The highest percentage of phosphorylation was observed during the competent larval stage. About 64 stage-specific phosphoprotein spots were detected in the competent stage, and 32 phosphoproteins were found to be significantly differentially expressed in the three stages. We identified 38 phosphoproteins, 10 of which were differentially expressed during metamorphosis. These phosphoproteins belonged to six categories of biological processes: (1) development, (2) cell differentiation and integrity, (3) transcription and translation, (4) metabolism, (5) protein-protein interaction and proteolysis, and (6) receptors and enzymes. Conclusion: This is the first study to report changes in phosphoprotein expression patterns during the metamorphosis of the marine polychaete P. vexillosa. The higher degree of phosphorylation during the process of attaining competence to settle and metamorphose may be due to fast morphological transitions regulated by various mechanisms. Our data are consistent with previous studies showing a high percentage of phosphorylation during competency in the barnacle Balanus amphitrite and the bryozoan Bugula neritina. The identified phosphoproteins may play an important role during metamorphosis, and further studies on the location and functions of important proteins during metamorphosis are warranted. © 2011 Chandramouli et al; licensee BioMed Central Ltd.en
dc.description.sponsorshipThe authors thank Mr. Y. Zhang for his technical help in generating the 2-DE gels, and Dr. S. Arellano for critically reviewing the manuscript and Cherry Kwan for proof-reading the manuscript. This study was supported by an award from King Abdullah University of Science and Technology (SA-C0040/UK-C0016), a grant from the China Ocean Mineral Resource Research and Development Association (COMARRD06/07.Sc02), and grants from the Research Grants Council of the Hong Kong Special Administrative Region (N-HKUST602/09, 662408 and AoE/P-04/04-II) to P.-Y. Qian.en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/en
dc.subjectBalanus amphitriteen
dc.subjectBryozoaen
dc.subjectBugula neritinaen
dc.subjectPolychaetaen
dc.subjectPseudopolydoraen
dc.subjectSpionidaen
dc.subjectThoracicaen
dc.subjectphosphoproteinen
dc.subjectproteomeen
dc.subjectaffinity chromatographyen
dc.subjectchemistryen
dc.subjectgrowth, development and agingen
dc.subjecthistologyen
dc.subjectlarvaen
dc.subjectmass spectrometryen
dc.subjectmetamorphosisen
dc.subjectmethodologyen
dc.subjectphysiologyen
dc.subjectPolychaetaen
dc.subjectprotein processingen
dc.subjecttwo dimensional gel electrophoresisen
dc.subjectChromatography, Affinityen
dc.subjectElectrophoresis, Gel, Two-Dimensionalen
dc.subjectLarvaen
dc.subjectMass Spectrometryen
dc.subjectMetamorphosis, Biologicalen
dc.subjectPhosphoproteinsen
dc.subjectPolychaetaen
dc.subjectProtein Processing, Post-Translationalen
dc.subjectProteomeen
dc.titlePhosphoproteome analysis during larval development and metamorphosis in the spionid polychaete Pseudopolydora vexillosaen
dc.typeArticleen
dc.identifier.journalBMC Developmental Biologyen
dc.identifier.pmcidPMC3115903en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionKAUST Global Collaborative Research Program, Division of Life Science, Hong Kong University of Science and Technology, Hong Kong SAR, Chinaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorWang, Haoen
kaust.authorChandramouli, Kondethimmanahallien
kaust.authorMok, FloraSyen
kaust.authorQian, Peiyuanen
kaust.grant.numberSA-C0040en
kaust.grant.numberUK-C0016en
kaust.grant.programKAUST Global Collaborative Research Programen
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