Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa

Handle URI:
http://hdl.handle.net/10754/334579
Title:
Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa
Authors:
Mok, Flora SY; Thiyagarajan, Vengatesen; Qian, Pei-Yuan
Abstract:
Background: While the larval-juvenile transition (metamorphosis) in the spionid polychaete Pseudopolydora vexillosa involves gradual morphological changes and does not require substantial development of juvenile organs, the opposite occurs in the barnacle Balanus amphitrite. We hypothesized that the proteome changes during metamorphosis in the spionids are less drastic than that in the barnacles. To test this, proteomes of pre-competent larvae, competent larvae (ready to metamorphose), and juveniles of P. vexillosa were compared using 2-dimensional gel electrophoresis (2-DE), and they were then compared to those of the barnacle.Results: Unlike the significant changes found during barnacle metamorphosis, proteomes of competent P. vexillosa larvae were more similar to those of their juveniles. Pre-competent larvae had significantly fewer protein spots (384 spots), while both competent larvae and juveniles expressed about 660 protein spots each. Proteins up-regulated during competence identified by MALDI-TOF/TOF analysis included a molecular chaperon (calreticulin), a signal transduction regulator (tyrosin activation protein), and a tissue-remodeling enzyme (metallopeptidase).Conclusions: This was the first time to study the protein expression patterns during the metamorphosis of a marine polychaete and to compare the proteomes of marine invertebrates that have different levels of morphological changes during metamorphosis. The findings provide promising initial steps towards the development of a proteome database for marine invertebrate metamorphosis, thus deciphering the possible mechanisms underlying larval metamorphosis in non-model marine organisms. © 2009 Mok et al; licensee BioMed Central Ltd.
Citation:
Mok FS, Thiyagarajan V, Qian P-Y (2009) Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa. Proteome Science 7: 44. doi:10.1186/1477-5956-7-44.
Publisher:
Springer Nature
Journal:
Proteome Science
Issue Date:
14-Dec-2009
DOI:
10.1186/1477-5956-7-44
PubMed ID:
20003395
PubMed Central ID:
PMC2803448
Type:
Article
ISSN:
1477-5956
Sponsors:
We are grateful to 3 anonymous reviewers for their constructive suggestions in improving the manuscript. We thank Dr. Priscilla Leung and Mr. Jack Man (Genome Research Centre, HKU) for MS analysis. We are also thankful to Dr. Kondethimma H. Chandramouli, Dr. Shawn M. Arellano, Dr. Olivia Ambrogio, and Dr. On On Lee for proof-reading the manuscript. This study was supported by the CAS/SAFEA International Partnership Program for Creative Research Teams, a research grant (GRF #662408) from the HKSAR Government, and KAUST Global Partnership Program awarded to PY Qian.
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DC FieldValue Language
dc.contributor.authorMok, Flora SYen
dc.contributor.authorThiyagarajan, Vengatesenen
dc.contributor.authorQian, Pei-Yuanen
dc.date.accessioned2014-11-11T14:30:34Z-
dc.date.available2014-11-11T14:30:34Z-
dc.date.issued2009-12-14en
dc.identifier.citationMok FS, Thiyagarajan V, Qian P-Y (2009) Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa. Proteome Science 7: 44. doi:10.1186/1477-5956-7-44.en
dc.identifier.issn1477-5956en
dc.identifier.pmid20003395en
dc.identifier.doi10.1186/1477-5956-7-44en
dc.identifier.urihttp://hdl.handle.net/10754/334579en
dc.description.abstractBackground: While the larval-juvenile transition (metamorphosis) in the spionid polychaete Pseudopolydora vexillosa involves gradual morphological changes and does not require substantial development of juvenile organs, the opposite occurs in the barnacle Balanus amphitrite. We hypothesized that the proteome changes during metamorphosis in the spionids are less drastic than that in the barnacles. To test this, proteomes of pre-competent larvae, competent larvae (ready to metamorphose), and juveniles of P. vexillosa were compared using 2-dimensional gel electrophoresis (2-DE), and they were then compared to those of the barnacle.Results: Unlike the significant changes found during barnacle metamorphosis, proteomes of competent P. vexillosa larvae were more similar to those of their juveniles. Pre-competent larvae had significantly fewer protein spots (384 spots), while both competent larvae and juveniles expressed about 660 protein spots each. Proteins up-regulated during competence identified by MALDI-TOF/TOF analysis included a molecular chaperon (calreticulin), a signal transduction regulator (tyrosin activation protein), and a tissue-remodeling enzyme (metallopeptidase).Conclusions: This was the first time to study the protein expression patterns during the metamorphosis of a marine polychaete and to compare the proteomes of marine invertebrates that have different levels of morphological changes during metamorphosis. The findings provide promising initial steps towards the development of a proteome database for marine invertebrate metamorphosis, thus deciphering the possible mechanisms underlying larval metamorphosis in non-model marine organisms. © 2009 Mok et al; licensee BioMed Central Ltd.en
dc.description.sponsorshipWe are grateful to 3 anonymous reviewers for their constructive suggestions in improving the manuscript. We thank Dr. Priscilla Leung and Mr. Jack Man (Genome Research Centre, HKU) for MS analysis. We are also thankful to Dr. Kondethimma H. Chandramouli, Dr. Shawn M. Arellano, Dr. Olivia Ambrogio, and Dr. On On Lee for proof-reading the manuscript. This study was supported by the CAS/SAFEA International Partnership Program for Creative Research Teams, a research grant (GRF #662408) from the HKSAR Government, and KAUST Global Partnership Program awarded to PY Qian.en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.rights.urihttp://creativecommons.org/licenses/by/2.0en
dc.subjectcalreticulinen
dc.subjectmetalloproteinaseen
dc.subjectproteomeen
dc.subjectbarnacleen
dc.subjectcomparative studyen
dc.subjectcontrolled studyen
dc.subjectjuvenile animalen
dc.subjectlarvaen
dc.subjectlarval developmenten
dc.subjectmarine speciesen
dc.subjectmatrix assisted laser desorption ionization time of flight mass spectrometryen
dc.subjectmetamorphosisen
dc.subjectnucleotide sequenceen
dc.subjectPolychaetaen
dc.subjectprotein analysisen
dc.subjectprotein expressionen
dc.subjectproteomicsen
dc.subjectsignal transductionen
dc.subjecttwo dimensional gel electrophoresisen
dc.subjectupregulationen
dc.subjectBalanus amphitriteen
dc.subjectInvertebrataen
dc.subjectPolychaetaen
dc.subjectPseudopolydoraen
dc.subjectSpionidaen
dc.subjectThoracicaen
dc.titleProteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosaen
dc.typeArticleen
dc.identifier.journalProteome Scienceen
dc.identifier.pmcidPMC2803448en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionSwire Institute of Marine Science/School of Biological Sciences, The University of Hong Kong, Hong Kong SAR, Chinaen
dc.contributor.institutionCAS/SAFEA International Partnership Program for Creative Research Teams, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Chinaen
dc.contributor.institutionKAUST Global Partnership Program, Department of Biology/Atmospheric, Marine and Coastal Environment Program, The Hong Kong University of Science and Technology, Hong Kong SAR, Chinaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorMok, Flora SYen
kaust.authorQian, Pei-Yuanen
kaust.grant.programKAUST Global Partnership Programen
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