Aqueous Extracts of the Marine Brown Alga Lobophora variegata Inhibit HIV-1 Infection at the Level of Virus Entry into Cells

Handle URI:
http://hdl.handle.net/10754/334578
Title:
Aqueous Extracts of the Marine Brown Alga Lobophora variegata Inhibit HIV-1 Infection at the Level of Virus Entry into Cells
Authors:
Kremb, Stephan; Helfer, Markus; Kraus, Birgit; Wolff, Horst; Wild, Christian; Schneider, Martha; Voolstra, Christian R. ( 0000-0003-4555-3795 ) ; Brack-Werner, Ruth
Abstract:
In recent years, marine algae have emerged as a rich and promising source of molecules with potent activities against various human pathogens. The widely distributed brown alga Lobophora variegata that is often associated with tropical coral reefs exerts strong antibacterial and antiprotozoal effects, but so far has not been associated with specific anti-viral activities. This study investigated potential HIV-1 inhibitory activity of L. variegata collected from different geographical regions, using a cell-based full replication HIV-1 reporter assay. Aqueous L. variegata extracts showed strong inhibitory effects on several HIV-1 strains, including drug-resistant and primary HIV-1 isolates, and protected even primary cells (PBMC) from HIV-1-infection. Anti-viral potency was related to ecological factors and showed clear differences depending on light exposition or epiphyte growth. Assays addressing early events of the HIV-1 replication cycle indicated that L. variegata extracts inhibited entry of HIV-1 into cells at a pre-fusion step possibly by impeding mobility of virus particles. Further characterization of the aqueous extract demonstrated that even high doses had only moderate effects on viability of cultured and primary cells (PBMCs). Imaging-based techniques revealed extract effects on the plasma membrane and actin filaments as well as induction of apoptosis at concentrations exceeding EC50 of anti-HIV-1 activity by more than 400 fold. In summary, we show for the first time that L. variegata extracts inhibit HIV-1 entry, thereby suggesting this alga as promising source for the development of novel HIV-1 inhibitors.
KAUST Department:
Red Sea Research Center (RSRC)
Citation:
Kremb S, Helfer M, Kraus B, Wolff H, Wild C, et al. (2014) Aqueous Extracts of the Marine Brown Alga Lobophora variegata Inhibit HIV-1 Infection at the Level of Virus Entry into Cells. PLoS ONE 9: e103895. doi:10.1371/journal.pone.0103895.
Publisher:
Public Library of Science (PLoS)
Journal:
PLoS ONE
Issue Date:
21-Aug-2014
DOI:
10.1371/journal.pone.0103895
PubMed ID:
25144758
PubMed Central ID:
PMC4140698
Type:
Article
ISSN:
1932-6203
Appears in Collections:
Articles; Red Sea Research Center (RSRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorKremb, Stephanen
dc.contributor.authorHelfer, Markusen
dc.contributor.authorKraus, Birgiten
dc.contributor.authorWolff, Horsten
dc.contributor.authorWild, Christianen
dc.contributor.authorSchneider, Marthaen
dc.contributor.authorVoolstra, Christian R.en
dc.contributor.authorBrack-Werner, Ruthen
dc.date.accessioned2014-11-11T14:30:32Z-
dc.date.available2014-11-11T14:30:32Z-
dc.date.issued2014-08-21en
dc.identifier.citationKremb S, Helfer M, Kraus B, Wolff H, Wild C, et al. (2014) Aqueous Extracts of the Marine Brown Alga Lobophora variegata Inhibit HIV-1 Infection at the Level of Virus Entry into Cells. PLoS ONE 9: e103895. doi:10.1371/journal.pone.0103895.en
dc.identifier.issn1932-6203en
dc.identifier.pmid25144758en
dc.identifier.doi10.1371/journal.pone.0103895en
dc.identifier.urihttp://hdl.handle.net/10754/334578en
dc.description.abstractIn recent years, marine algae have emerged as a rich and promising source of molecules with potent activities against various human pathogens. The widely distributed brown alga Lobophora variegata that is often associated with tropical coral reefs exerts strong antibacterial and antiprotozoal effects, but so far has not been associated with specific anti-viral activities. This study investigated potential HIV-1 inhibitory activity of L. variegata collected from different geographical regions, using a cell-based full replication HIV-1 reporter assay. Aqueous L. variegata extracts showed strong inhibitory effects on several HIV-1 strains, including drug-resistant and primary HIV-1 isolates, and protected even primary cells (PBMC) from HIV-1-infection. Anti-viral potency was related to ecological factors and showed clear differences depending on light exposition or epiphyte growth. Assays addressing early events of the HIV-1 replication cycle indicated that L. variegata extracts inhibited entry of HIV-1 into cells at a pre-fusion step possibly by impeding mobility of virus particles. Further characterization of the aqueous extract demonstrated that even high doses had only moderate effects on viability of cultured and primary cells (PBMCs). Imaging-based techniques revealed extract effects on the plasma membrane and actin filaments as well as induction of apoptosis at concentrations exceeding EC50 of anti-HIV-1 activity by more than 400 fold. In summary, we show for the first time that L. variegata extracts inhibit HIV-1 entry, thereby suggesting this alga as promising source for the development of novel HIV-1 inhibitors.en
dc.language.isoenen
dc.publisherPublic Library of Science (PLoS)en
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rightsArchived with thanks to PLoS ONEen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleAqueous Extracts of the Marine Brown Alga Lobophora variegata Inhibit HIV-1 Infection at the Level of Virus Entry into Cellsen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalPLoS ONEen
dc.identifier.pmcidPMC4140698en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionInstitute of Virology, Helmholtz Zentrum Muenchen, Neuherberg, Germanyen
dc.contributor.institutionInstitute of Pharmacy, University of Regensburg, Regensburg, Germanyen
dc.contributor.institutionCarl Zeiss Microscopy GmbH, Goettingen, Germanyen
dc.contributor.institutionCoral Reef Ecology Group (CORE), Leibniz Center for Tropical Marine Ecology (ZMT), Bremen, Germanyen
dc.contributor.institutionUniversity of Bremen, Faculty of Biology and Chemistry, Bremen, Germanyen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorVoolstra, Christian R.en

Related articles on PubMed

This item is licensed under a Creative Commons License
Creative Commons
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.