Regenerant arabidopsis lineages display a distinct genome-wide spectrum of mutations conferring variant phenotypes

Handle URI:
http://hdl.handle.net/10754/334570
Title:
Regenerant arabidopsis lineages display a distinct genome-wide spectrum of mutations conferring variant phenotypes
Authors:
Jiang, Caifu; Mithani, Aziz; Gan, Xiangchao; Belfield, Eric J.; Klingler, John P.; Zhu, Jian-Kang ( 0000-0001-5134-731X ) ; Ragoussis, Jiannis; Mott, Richard; Harberd, Nicholas P.
Abstract:
Multicellular organisms can be regenerated from totipotent differentiated somatic cell or nuclear founders [1-3]. Organisms regenerated from clonally related isogenic founders might a priori have been expected to be phenotypically invariant. However, clonal regenerant animals display variant phenotypes caused by defective epigenetic reprogramming of gene expression [2], and clonal regenerant plants exhibit poorly understood heritable phenotypic ("somaclonal") variation [4-7]. Here we show that somaclonal variation in regenerant Arabidopsis lineages is associated with genome-wide elevation in DNA sequence mutation rate. We also show that regenerant mutations comprise a distinctive molecular spectrum of base substitutions, insertions, and deletions that probably results from decreased DNA repair fidelity. Finally, we show that while regenerant base substitutions are a likely major genetic cause of the somaclonal variation of regenerant Arabidopsis lineages, transposon movement is unlikely to contribute substantially to that variation. We conclude that the phenotypic variation of regenerant plants, unlike that of regenerant animals, is substantially due to DNA sequence mutation. 2011 Elsevier Ltd. All rights reserved.
KAUST Department:
Plant Stress Genomics Research Lab
Citation:
Jiang C, Mithani A, Gan X, Belfield EJ, Klingler JP, et al. (2011) Regenerant Arabidopsis Lineages Display a Distinct Genome-Wide Spectrum of Mutations Conferring Variant Phenotypes. Current Biology 21: 1385-1390. doi:10.1016/j.cub.2011.07.002.
Publisher:
Elsevier BV
Journal:
Current Biology
Issue Date:
28-Jul-2011
DOI:
10.1016/j.cub.2011.07.002
PubMed ID:
21802297
PubMed Central ID:
PMC3162137
Type:
Article
ISSN:
09609822
Appears in Collections:
Articles

Full metadata record

DC FieldValue Language
dc.contributor.authorJiang, Caifuen
dc.contributor.authorMithani, Azizen
dc.contributor.authorGan, Xiangchaoen
dc.contributor.authorBelfield, Eric J.en
dc.contributor.authorKlingler, John P.en
dc.contributor.authorZhu, Jian-Kangen
dc.contributor.authorRagoussis, Jiannisen
dc.contributor.authorMott, Richarden
dc.contributor.authorHarberd, Nicholas P.en
dc.date.accessioned2014-11-11T14:30:04Z-
dc.date.available2014-11-11T14:30:04Z-
dc.date.issued2011-07-28en
dc.identifier.citationJiang C, Mithani A, Gan X, Belfield EJ, Klingler JP, et al. (2011) Regenerant Arabidopsis Lineages Display a Distinct Genome-Wide Spectrum of Mutations Conferring Variant Phenotypes. Current Biology 21: 1385-1390. doi:10.1016/j.cub.2011.07.002.en
dc.identifier.issn09609822en
dc.identifier.pmid21802297en
dc.identifier.doi10.1016/j.cub.2011.07.002en
dc.identifier.urihttp://hdl.handle.net/10754/334570en
dc.description.abstractMulticellular organisms can be regenerated from totipotent differentiated somatic cell or nuclear founders [1-3]. Organisms regenerated from clonally related isogenic founders might a priori have been expected to be phenotypically invariant. However, clonal regenerant animals display variant phenotypes caused by defective epigenetic reprogramming of gene expression [2], and clonal regenerant plants exhibit poorly understood heritable phenotypic ("somaclonal") variation [4-7]. Here we show that somaclonal variation in regenerant Arabidopsis lineages is associated with genome-wide elevation in DNA sequence mutation rate. We also show that regenerant mutations comprise a distinctive molecular spectrum of base substitutions, insertions, and deletions that probably results from decreased DNA repair fidelity. Finally, we show that while regenerant base substitutions are a likely major genetic cause of the somaclonal variation of regenerant Arabidopsis lineages, transposon movement is unlikely to contribute substantially to that variation. We conclude that the phenotypic variation of regenerant plants, unlike that of regenerant animals, is substantially due to DNA sequence mutation. 2011 Elsevier Ltd. All rights reserved.en
dc.language.isoenen
dc.publisherElsevier BVen
dc.rightsOpen Access funded by Wellcome Trusten
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/en
dc.subjectArabidopsisen
dc.subjectgeneticsen
dc.subjecthistologyen
dc.subjectmolecular geneticsen
dc.subjectmutationen
dc.subjectmutation rateen
dc.subjectnucleotide sequenceen
dc.subjectphenotypeen
dc.subjectphysiologyen
dc.subjectplant genomeen
dc.subjectregenerationen
dc.subjectsequence alignmenten
dc.subjecttransposonen
dc.subjectArabidopsisen
dc.subjectBase Sequenceen
dc.subjectDNA Mutational Analysisen
dc.subjectDNA Transposable Elementsen
dc.subjectGenome, Planten
dc.subjectMolecular Sequence Dataen
dc.subjectMutationen
dc.subjectMutation Rateen
dc.subjectPhenotypeen
dc.subjectRegenerationen
dc.subjectSequence Alignmenten
dc.subjectAnimaliaen
dc.subjectArabidopsisen
dc.titleRegenerant arabidopsis lineages display a distinct genome-wide spectrum of mutations conferring variant phenotypesen
dc.typeArticleen
dc.contributor.departmentPlant Stress Genomics Research Laben
dc.identifier.journalCurrent Biologyen
dc.identifier.pmcidPMC3162137en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, United Kingdomen
dc.contributor.institutionWellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, United Kingdomen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorKlingler, Johnen
kaust.authorZhu, Jian-Kangen

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