Timing, coordination, and rhythm: Acrobatics at the DNA replication fork

Handle URI:
http://hdl.handle.net/10754/334568
Title:
Timing, coordination, and rhythm: Acrobatics at the DNA replication fork
Authors:
Hamdan, Samir ( 0000-0001-5192-1852 ) ; van Oijen, Antoine M.
Abstract:
In DNA replication, the antiparallel nature of the parental duplex imposes certain constraints on the activity of the DNA polymerases that synthesize new DNA. The leading-strand polymerase advances in a continuous fashion, but the lagging-strand polymerase is forced to restart at short intervals. In several prokaryotic systems studied so far, this problem is solved by the formation of a loop in the lagging strand of the replication fork to reorient the lagging-strand DNA polymerase so that it advances in parallel with the leading-strand polymerase. The replication loop grows and shrinks during each cycle of Okazaki fragment synthesis. The timing of Okazaki fragment synthesis and loop formation is determined by a subtle interplay of enzymatic activities at the fork. Recent developments in single-molecule techniques have enabled the direct observation of these processes and have greatly contributed to a better understanding of the dynamic nature of the replication fork. Here, we will review recent experimental advances, present the current models, and discuss some of the exciting developments in the field. 2010 by The American Society for Biochemistry and Molecular Biology, Inc.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Hamdan SM, van Oijen AM (2010) Timing, Coordination, and Rhythm: Acrobatics at the DNA Replication Fork. Journal of Biological Chemistry 285: 18979-18983. doi:10.1074/jbc.R109.022939.
Publisher:
American Society for Biochemistry & Molecular Biology (ASBMB)
Journal:
Journal of Biological Chemistry
Issue Date:
9-Apr-2010
DOI:
10.1074/jbc.R109.022939
PubMed ID:
20382733
PubMed Central ID:
PMC2885174
Type:
Article
ISSN:
00219258
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorHamdan, Samiren
dc.contributor.authorvan Oijen, Antoine M.en
dc.date.accessioned2014-11-11T14:29:58Z-
dc.date.available2014-11-11T14:29:58Z-
dc.date.issued2010-04-09en
dc.identifier.citationHamdan SM, van Oijen AM (2010) Timing, Coordination, and Rhythm: Acrobatics at the DNA Replication Fork. Journal of Biological Chemistry 285: 18979-18983. doi:10.1074/jbc.R109.022939.en
dc.identifier.issn00219258en
dc.identifier.pmid20382733en
dc.identifier.doi10.1074/jbc.R109.022939en
dc.identifier.urihttp://hdl.handle.net/10754/334568en
dc.description.abstractIn DNA replication, the antiparallel nature of the parental duplex imposes certain constraints on the activity of the DNA polymerases that synthesize new DNA. The leading-strand polymerase advances in a continuous fashion, but the lagging-strand polymerase is forced to restart at short intervals. In several prokaryotic systems studied so far, this problem is solved by the formation of a loop in the lagging strand of the replication fork to reorient the lagging-strand DNA polymerase so that it advances in parallel with the leading-strand polymerase. The replication loop grows and shrinks during each cycle of Okazaki fragment synthesis. The timing of Okazaki fragment synthesis and loop formation is determined by a subtle interplay of enzymatic activities at the fork. Recent developments in single-molecule techniques have enabled the direct observation of these processes and have greatly contributed to a better understanding of the dynamic nature of the replication fork. Here, we will review recent experimental advances, present the current models, and discuss some of the exciting developments in the field. 2010 by The American Society for Biochemistry and Molecular Biology, Inc.en
dc.language.isoenen
dc.publisherAmerican Society for Biochemistry & Molecular Biology (ASBMB)en
dc.rightsAuthor's Choice—Final version full access. Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) applies to Author Choice Articlesen
dc.rightsArchived with thanks to Journal of Biological Chemistryen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/en
dc.subjectCurrent modelsen
dc.subjectDirect observationen
dc.subjectDNA polymeraseen
dc.subjectDNA replication forksen
dc.subjectDNA replicationsen
dc.subjectDynamic natureen
dc.subjectEnzymatic activitiesen
dc.subjectLagging-stranden
dc.subjectLoop formationen
dc.subjectOkazaki fragmentsen
dc.subjectProkaryotic systemen
dc.subjectReplication forken
dc.subjectShort-intervalen
dc.subjectSingle-molecule techniqueen
dc.subjectCoordination reactionsen
dc.subjectDNAen
dc.subjectGenesen
dc.subjectTime measurementen
dc.subjectPolymersen
dc.subjectDNA polymeraseen
dc.subjectreplisomeen
dc.subjectbacteriophage T7en
dc.subjectDNA denaturationen
dc.subjectDNA replicationen
dc.subjectDNA replication timingen
dc.subjectDNA structureen
dc.subjectDNA synthesisen
dc.subjectelectron microscopyen
dc.subjectenzyme activityen
dc.subjectmolecular dynamicsen
dc.subjectnonhumanen
dc.subjectpriority journalen
dc.subjectprokaryoteen
dc.subjectprotein protein interactionen
dc.subjectshort surveyen
dc.subjectsignal transductionen
dc.subjectBacteriophage T7en
dc.subjectBiochemistryen
dc.subjectDNAen
dc.subjectDNA Primaseen
dc.subjectDNA Replicationen
dc.subjectDNA-Directed DNA Polymeraseen
dc.subjectEnzymesen
dc.subjectEscherichia colien
dc.subjectMicroscopy, Electronen
dc.subjectNucleic Acidsen
dc.subjectProteinsen
dc.subjectRNAen
dc.subjectThioredoxinsen
dc.subjectProkaryotaen
dc.titleTiming, coordination, and rhythm: Acrobatics at the DNA replication forken
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalJournal of Biological Chemistryen
dc.identifier.pmcidPMC2885174en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, United Statesen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorHamdan, Samiren

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