The Population Structure of Vibrio cholerae from the Chandigarh Region of Northern India

Handle URI:
http://hdl.handle.net/10754/334549
Title:
The Population Structure of Vibrio cholerae from the Chandigarh Region of Northern India
Authors:
Abd El Ghany, Moataz; Chander, Jagadish; Mutreja, Ankur; Rashid, Mamoon ( 0000-0003-4851-4994 ) ; Hill-Cawthorne, Grant A. ( 0000-0002-3828-5473 ) ; Ali, Shahjahan; Naeem, Raeece ( 0000-0003-1734-1007 ) ; Thomson, Nicholas R.; Dougan, Gordon; Pain, Arnab ( 0000-0002-1755-2819 )
Abstract:
Background:Cholera infection continues to be a threat to global public health. The current cholera pandemic associated with Vibrio cholerae El Tor has now been ongoing for over half a century.Methodology/Principal Findings:Thirty-eight V. cholerae El Tor isolates associated with a cholera outbreak in 2009 from the Chandigarh region of India were characterised by a combination of microbiology, molecular typing and whole-genome sequencing. The genomic analysis indicated that two clones of V. cholera circulated in the region and caused disease during this time. These clones fell into two distinct sub-clades that map independently onto wave 3 of the phylogenetic tree of seventh pandemic V. cholerae El Tor. Sequence analyses of the cholera toxin gene, the Vibrio seventh Pandemic Island II (VSPII) and SXT element correlated with this phylogenetic position of the two clades on the El Tor tree. The clade 2 isolates, characterized by a drug-resistant profile and the expression of a distinct cholera toxin, are closely related to the recent V. cholerae isolated elsewhere, including Haiti, but fell on a distinct branch of the tree, showing they were independent outbreaks. Multi-Locus Sequence Typing (MLST) distinguishes two sequence types among the 38 isolates, that did not correspond to the clades defined by whole-genome sequencing. Multi-Locus Variable-length tandem-nucleotide repeat Analysis (MLVA) identified 16 distinct clusters.Conclusions/Significance:The use of whole-genome sequencing enabled the identification of two clones of V. cholerae that circulated during the 2009 Chandigarh outbreak. These clones harboured a similar structure of ICEVchHai1 but differed mainly in the structure of CTX phage and VSPII. The limited capacity of MLST and MLVA to discriminate between the clones that circulated in the 2009 Chandigarh outbreak highlights the value of whole-genome sequencing as a route to the identification of further genetic markers to subtype V. cholerae isolates. © 2014 Abd El Ghany et al.
KAUST Department:
Computational Bioscience Research Center (CBRC); Pathogen Genomics Laboratory; Biosciences Core Lab
Citation:
Abd El Ghany M, Chander J, Mutreja A, Rashid M, Hill-Cawthorne GA, et al. (2014) The Population Structure of Vibrio cholerae from the Chandigarh Region of Northern India. PLoS Negl Trop Dis 8: e2981. doi:10.1371/journal.pntd.0002981.
Publisher:
Public Library of Science (PLoS)
Journal:
PLoS Neglected Tropical Diseases
Issue Date:
24-Jul-2014
DOI:
10.1371/journal.pntd.0002981
PubMed ID:
25058483
PubMed Central ID:
PMC4109905
Type:
Article
ISSN:
19352727
Appears in Collections:
Articles; Biosciences Core Lab; Computational Bioscience Research Center (CBRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorAbd El Ghany, Moatazen
dc.contributor.authorChander, Jagadishen
dc.contributor.authorMutreja, Ankuren
dc.contributor.authorRashid, Mamoonen
dc.contributor.authorHill-Cawthorne, Grant A.en
dc.contributor.authorAli, Shahjahanen
dc.contributor.authorNaeem, Raeeceen
dc.contributor.authorThomson, Nicholas R.en
dc.contributor.authorDougan, Gordonen
dc.contributor.authorPain, Arnaben
dc.date.accessioned2014-11-11T14:29:16Z-
dc.date.available2014-11-11T14:29:16Z-
dc.date.issued2014-07-24en
dc.identifier.citationAbd El Ghany M, Chander J, Mutreja A, Rashid M, Hill-Cawthorne GA, et al. (2014) The Population Structure of Vibrio cholerae from the Chandigarh Region of Northern India. PLoS Negl Trop Dis 8: e2981. doi:10.1371/journal.pntd.0002981.en
dc.identifier.issn19352727en
dc.identifier.pmid25058483en
dc.identifier.doi10.1371/journal.pntd.0002981en
dc.identifier.urihttp://hdl.handle.net/10754/334549en
dc.description.abstractBackground:Cholera infection continues to be a threat to global public health. The current cholera pandemic associated with Vibrio cholerae El Tor has now been ongoing for over half a century.Methodology/Principal Findings:Thirty-eight V. cholerae El Tor isolates associated with a cholera outbreak in 2009 from the Chandigarh region of India were characterised by a combination of microbiology, molecular typing and whole-genome sequencing. The genomic analysis indicated that two clones of V. cholera circulated in the region and caused disease during this time. These clones fell into two distinct sub-clades that map independently onto wave 3 of the phylogenetic tree of seventh pandemic V. cholerae El Tor. Sequence analyses of the cholera toxin gene, the Vibrio seventh Pandemic Island II (VSPII) and SXT element correlated with this phylogenetic position of the two clades on the El Tor tree. The clade 2 isolates, characterized by a drug-resistant profile and the expression of a distinct cholera toxin, are closely related to the recent V. cholerae isolated elsewhere, including Haiti, but fell on a distinct branch of the tree, showing they were independent outbreaks. Multi-Locus Sequence Typing (MLST) distinguishes two sequence types among the 38 isolates, that did not correspond to the clades defined by whole-genome sequencing. Multi-Locus Variable-length tandem-nucleotide repeat Analysis (MLVA) identified 16 distinct clusters.Conclusions/Significance:The use of whole-genome sequencing enabled the identification of two clones of V. cholerae that circulated during the 2009 Chandigarh outbreak. These clones harboured a similar structure of ICEVchHai1 but differed mainly in the structure of CTX phage and VSPII. The limited capacity of MLST and MLVA to discriminate between the clones that circulated in the 2009 Chandigarh outbreak highlights the value of whole-genome sequencing as a route to the identification of further genetic markers to subtype V. cholerae isolates. © 2014 Abd El Ghany et al.en
dc.language.isoenen
dc.publisherPublic Library of Science (PLoS)en
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rightsArchived with thanks to PLoS Neglected Tropical Diseasesen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleThe Population Structure of Vibrio cholerae from the Chandigarh Region of Northern Indiaen
dc.typeArticleen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.contributor.departmentPathogen Genomics Laboratoryen
dc.contributor.departmentBiosciences Core Laben
dc.identifier.journalPLoS Neglected Tropical Diseasesen
dc.identifier.pmcidPMC4109905en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Microbiology, Government Medical College Hospital (GMCH), Chandigarh, Indiaen
dc.contributor.institutionThe Wellcome Trust Sanger Institute (WTSI), The Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdomen
dc.contributor.institutionMarie Bashir Institute for Infectious Diseases and Biosecurity, School of Public Health, University of Sydney, Sydney, Australiaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorAbd El Ghany, Moatazen
kaust.authorRashid, Mamoonen
kaust.authorHill-Cawthorne, Grant A.en
kaust.authorAli, Shahjahanen
kaust.authorNaeem, Raeeceen
kaust.authorPain, Arnaben

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