Bacterial profiling of White Plague Disease across corals and oceans indicates a conserved and distinct disease microbiome

Handle URI:
http://hdl.handle.net/10754/334532
Title:
Bacterial profiling of White Plague Disease across corals and oceans indicates a conserved and distinct disease microbiome
Authors:
Roder, C.; Arif, C.; Daniels, C.; Weil, E.; Voolstra, Christian R. ( 0000-0003-4555-3795 )
Abstract:
Coral diseases are characterized by microbial community shifts in coral mucus and tissue, but causes and consequences of these changes are vaguely understood due to the complexity and dynamics of coral-associated bacteria. We used 16S rRNA gene microarrays to assay differences in bacterial assemblages of healthy and diseased colonies displaying White Plague Disease (WPD) signs from two closely related Caribbean coral species, Orbicella faveolata and Orbicella franksi. Analysis of differentially abundant operational taxonomic units (OTUs) revealed strong differences between healthy and diseased specimens, but not between coral species. A subsequent comparison to data from two Indo-Pacific coral species (Pavona duerdeni and Porites lutea) revealed distinct microbial community patterns associated with ocean basin, coral species and health state. Coral species were clearly separated by site, but also, the relatedness of the underlying bacterial community structures resembled the phylogenetic relationship of the coral hosts. In diseased samples, bacterial richness increased and putatively opportunistic bacteria were consistently more abundant highlighting the role of opportunistic conditions in structuring microbial community patterns during disease. Our comparative analysis shows that it is possible to derive conserved bacterial footprints of diseased coral holobionts that might help in identifying key bacterial species related to the underlying etiopathology. Furthermore, our data demonstrate that similar-appearing disease phenotypes produce microbial community patterns that are consistent over coral species and oceans, irrespective of the putative underlying pathogen. Consequently, profiling coral diseases by microbial community structure over multiple coral species might allow the development of a comparative disease framework that can inform on cause and relatedness of coral diseases. 2013 The Authors Molecular Ecology John Wiley & Sons Ltd.
KAUST Department:
Red Sea Research Center (RSRC)
Citation:
Roder C, Arif C, Daniels C, Weil E, Voolstra CR (2014) Bacterial profiling of White Plague Disease across corals and oceans indicates a conserved and distinct disease microbiome. Molecular Ecology 23: 965-974. doi:10.1111/mec.12638.
Publisher:
Wiley
Journal:
Molecular Ecology
Issue Date:
29-Jan-2014
DOI:
10.1111/mec.12638
PubMed ID:
24350609
PubMed Central ID:
PMC4285310
Type:
Article
ISSN:
09621083
Appears in Collections:
Articles; Red Sea Research Center (RSRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorRoder, C.en
dc.contributor.authorArif, C.en
dc.contributor.authorDaniels, C.en
dc.contributor.authorWeil, E.en
dc.contributor.authorVoolstra, Christian R.en
dc.date.accessioned2014-11-11T14:28:42Z-
dc.date.available2014-11-11T14:28:42Z-
dc.date.issued2014-01-29en
dc.identifier.citationRoder C, Arif C, Daniels C, Weil E, Voolstra CR (2014) Bacterial profiling of White Plague Disease across corals and oceans indicates a conserved and distinct disease microbiome. Molecular Ecology 23: 965-974. doi:10.1111/mec.12638.en
dc.identifier.issn09621083en
dc.identifier.pmid24350609en
dc.identifier.doi10.1111/mec.12638en
dc.identifier.urihttp://hdl.handle.net/10754/334532en
dc.description.abstractCoral diseases are characterized by microbial community shifts in coral mucus and tissue, but causes and consequences of these changes are vaguely understood due to the complexity and dynamics of coral-associated bacteria. We used 16S rRNA gene microarrays to assay differences in bacterial assemblages of healthy and diseased colonies displaying White Plague Disease (WPD) signs from two closely related Caribbean coral species, Orbicella faveolata and Orbicella franksi. Analysis of differentially abundant operational taxonomic units (OTUs) revealed strong differences between healthy and diseased specimens, but not between coral species. A subsequent comparison to data from two Indo-Pacific coral species (Pavona duerdeni and Porites lutea) revealed distinct microbial community patterns associated with ocean basin, coral species and health state. Coral species were clearly separated by site, but also, the relatedness of the underlying bacterial community structures resembled the phylogenetic relationship of the coral hosts. In diseased samples, bacterial richness increased and putatively opportunistic bacteria were consistently more abundant highlighting the role of opportunistic conditions in structuring microbial community patterns during disease. Our comparative analysis shows that it is possible to derive conserved bacterial footprints of diseased coral holobionts that might help in identifying key bacterial species related to the underlying etiopathology. Furthermore, our data demonstrate that similar-appearing disease phenotypes produce microbial community patterns that are consistent over coral species and oceans, irrespective of the putative underlying pathogen. Consequently, profiling coral diseases by microbial community structure over multiple coral species might allow the development of a comparative disease framework that can inform on cause and relatedness of coral diseases. 2013 The Authors Molecular Ecology John Wiley & Sons Ltd.en
dc.language.isoenen
dc.publisherWileyen
dc.rightsThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en
dc.rightsArchived with thanks to Molecular Ecologyen
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/en
dc.subject16S rRNA gene microarrayen
dc.subjectcoral diseaseen
dc.subjectmicrobial communityen
dc.subjectOrbicella faveolataen
dc.subjectOrbicella franksien
dc.subjectPavona duerdenien
dc.subjectPorites luteaen
dc.subjectWhite Plague Disease (WPD)en
dc.subjectWhite Plague-like Diseaseen
dc.subjectWhite Syndrome (WS)en
dc.subjectbacterial DNAen
dc.subjectRNA 16Sen
dc.subjectAnthozoaen
dc.subjectbacteriumen
dc.subjectCentral Americaen
dc.subjectclassificationen
dc.subjectgeneticsen
dc.subjectmicrobiologyen
dc.subjectmicrofloraen
dc.subjectphylogenyen
dc.subjectBacteriaen
dc.subjectCaribbean Regionen
dc.subjectDNA, Bacterialen
dc.subjectMicrobiotaen
dc.subjectPhylogenyen
dc.subjectRNA, Ribosomal, 16Sen
dc.titleBacterial profiling of White Plague Disease across corals and oceans indicates a conserved and distinct disease microbiomeen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalMolecular Ecologyen
dc.identifier.pmcidPMC4285310en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Marine Sciences, University of Puerto Rico, PO BOX 9000, Mayaguez PR 00680, United Statesen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorRoder, Corneliaen
kaust.authorArif, Chatchaniten
kaust.authorDaniels, Camille Arianen
kaust.authorVoolstra, Christian R.en

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