Novel polymorphic microsatellite markers developed for a common reef sponge, Stylissa carteri

Handle URI:
http://hdl.handle.net/10754/334514
Title:
Novel polymorphic microsatellite markers developed for a common reef sponge, Stylissa carteri
Authors:
Giles, E.C.; Saenz-Agudelo, P.; Berumen, Michael L. ( 0000-0003-2463-2742 ) ; Ravasi, Timothy ( 0000-0002-9950-465X )
Abstract:
Despite the ubiquitous role sponges play in reef ecosystem dynamics, little is known about population-level connectivity in these organisms. The general field of population genetics in sponges remains in its infancy. To date, microsatellite markers have only been developed for few sponge species and no sponge population genetics studies using microsatellites have been conducted in the Red Sea. Here, with the use of next-generation sequencing, we characterize 12 novel polymorphic loci for the common reef sponge, Stylissa carteri. The number of alleles per loci ranged between three and eight. Observed heterozygosity frequencies (Ho) ranged from 0.125 to 0.870, whereas expected (He) heterozygosity frequencies ranged from 0.119 to 0.812. Only one locus showed consistent deviations from Hardy-Weinberg equilibrium (HWE) in both populations and two loci consistently showed the possible presence of null alleles. No significant linkage disequilibrium was detected for any pairs of loci. These microsatellites will be of use for numerous ecological studies focused on this common and abundant sponge. 2013 The Author(s).
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Red Sea Research Center (RSRC)
Citation:
Giles EC, Saenz-Agudelo P, Berumen ML, Ravasi T (2013) Novel polymorphic microsatellite markers developed for a common reef sponge, Stylissa carteri. Marine Biodiversity 43: 237-241. doi:10.1007/s12526-013-0151-x.
Publisher:
Springer Verlag
Journal:
Marine Biodiversity
Issue Date:
4-Apr-2013
DOI:
10.1007/s12526-013-0151-x
Type:
Article
ISSN:
18671616
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorGiles, E.C.en
dc.contributor.authorSaenz-Agudelo, P.en
dc.contributor.authorBerumen, Michael L.en
dc.contributor.authorRavasi, Timothyen
dc.date.accessioned2014-11-11T14:27:55Z-
dc.date.available2014-11-11T14:27:55Z-
dc.date.issued2013-04-04en
dc.identifier.citationGiles EC, Saenz-Agudelo P, Berumen ML, Ravasi T (2013) Novel polymorphic microsatellite markers developed for a common reef sponge, Stylissa carteri. Marine Biodiversity 43: 237-241. doi:10.1007/s12526-013-0151-x.en
dc.identifier.issn18671616en
dc.identifier.doi10.1007/s12526-013-0151-xen
dc.identifier.urihttp://hdl.handle.net/10754/334514en
dc.description.abstractDespite the ubiquitous role sponges play in reef ecosystem dynamics, little is known about population-level connectivity in these organisms. The general field of population genetics in sponges remains in its infancy. To date, microsatellite markers have only been developed for few sponge species and no sponge population genetics studies using microsatellites have been conducted in the Red Sea. Here, with the use of next-generation sequencing, we characterize 12 novel polymorphic loci for the common reef sponge, Stylissa carteri. The number of alleles per loci ranged between three and eight. Observed heterozygosity frequencies (Ho) ranged from 0.125 to 0.870, whereas expected (He) heterozygosity frequencies ranged from 0.119 to 0.812. Only one locus showed consistent deviations from Hardy-Weinberg equilibrium (HWE) in both populations and two loci consistently showed the possible presence of null alleles. No significant linkage disequilibrium was detected for any pairs of loci. These microsatellites will be of use for numerous ecological studies focused on this common and abundant sponge. 2013 The Author(s).en
dc.language.isoenen
dc.publisherSpringer Verlagen
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/en
dc.subjectConnectivityen
dc.subjectMicrosatellitesen
dc.subjectPopulation geneticsen
dc.subjectSpongesen
dc.subjectStylissa carterien
dc.subjectalleleen
dc.subjectconnectivityen
dc.subjectevolutionary theoryen
dc.subjectgenetic analysisen
dc.subjectgenetic markeren
dc.subjectheterozygosityen
dc.subjectpolymorphismen
dc.subjectpopulation geneticsen
dc.subjectreefen
dc.subjectspongeen
dc.subjectIndian Oceanen
dc.subjectRed Sea [Indian Ocean]en
dc.titleNovel polymorphic microsatellite markers developed for a common reef sponge, Stylissa carterien
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalMarine Biodiversityen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionBiology Department, Woods Hole Oceanographic Institution, Woods Hole, MA, 02543, United Statesen
dc.contributor.institutionDepartment of Medicine, Division of Medical Genetics, University of California, San Diego, 9500 Gilman Drive La Jolla, San Diego, CA, 92093, United Statesen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorGiles, Emilyen
kaust.authorSaenz Agudelo, Pabloen
kaust.authorBerumen, Michael L.en
kaust.authorRavasi, Timothyen
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