Single-molecule diffusion and conformational dynamics by spatial integration of temporal fluctuations

Handle URI:
http://hdl.handle.net/10754/333764
Title:
Single-molecule diffusion and conformational dynamics by spatial integration of temporal fluctuations
Authors:
Bayoumi, Maged Fouad ( 0000-0002-6153-1089 ) ; Abadi, Maram ( 0000-0003-3802-8669 ) ; Habuchi, Satoshi ( 0000-0002-6663-2807 )
Abstract:
Single-molecule localization and tracking has been used to translate spatiotemporal information of individual molecules to map their diffusion behaviours. However, accurate analysis of diffusion behaviours and including other parameters, such as the conformation and size of molecules, remain as limitations to the method. Here, we report a method that addresses the limitations of existing single-molecular localization methods. The method is based on temporal tracking of the cumulative area occupied by molecules. These temporal fluctuations are tied to molecular size, rates of diffusion and conformational changes. By analysing fluorescent nanospheres and double-stranded DNA molecules of different lengths and topological forms, we demonstrate that our cumulative-area method surpasses the conventional single-molecule localization method in terms of the accuracy of determined diffusion coefficients. Furthermore, the cumulative-area method provides conformational relaxation times of structurally flexible chains along with diffusion coefficients, which together are relevant to work in a wide spectrum of scientific fields.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Serag, M. F. et al. Single-molecule diffusion and conformational dynamics by spatial integration of temporal fluctuations. Nat. Commun. 5:5123 doi: 10.1038/ncomms6123 (2014).
Publisher:
Nature Publishing Group
Journal:
Nature Communications
Issue Date:
6-Oct-2014
DOI:
10.1038/ncomms6123
PubMed ID:
25283876
PubMed Central ID:
PMC4205855
Type:
Article
ISSN:
2041-1723
Additional Links:
http://www.nature.com/doifinder/10.1038/ncomms6123
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorBayoumi, Maged Fouaden
dc.contributor.authorAbadi, Maramen
dc.contributor.authorHabuchi, Satoshien
dc.date.accessioned2014-11-06T07:21:30Z-
dc.date.available2014-11-06T07:21:30Z-
dc.date.issued2014-10-06en
dc.identifier.citationSerag, M. F. et al. Single-molecule diffusion and conformational dynamics by spatial integration of temporal fluctuations. Nat. Commun. 5:5123 doi: 10.1038/ncomms6123 (2014).en
dc.identifier.issn2041-1723en
dc.identifier.pmid25283876en
dc.identifier.doi10.1038/ncomms6123en
dc.identifier.urihttp://hdl.handle.net/10754/333764en
dc.description.abstractSingle-molecule localization and tracking has been used to translate spatiotemporal information of individual molecules to map their diffusion behaviours. However, accurate analysis of diffusion behaviours and including other parameters, such as the conformation and size of molecules, remain as limitations to the method. Here, we report a method that addresses the limitations of existing single-molecular localization methods. The method is based on temporal tracking of the cumulative area occupied by molecules. These temporal fluctuations are tied to molecular size, rates of diffusion and conformational changes. By analysing fluorescent nanospheres and double-stranded DNA molecules of different lengths and topological forms, we demonstrate that our cumulative-area method surpasses the conventional single-molecule localization method in terms of the accuracy of determined diffusion coefficients. Furthermore, the cumulative-area method provides conformational relaxation times of structurally flexible chains along with diffusion coefficients, which together are relevant to work in a wide spectrum of scientific fields.en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.relation.urlhttp://www.nature.com/doifinder/10.1038/ncomms6123en
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectPhysical sciencesen
dc.subjectAnalytical chemistryen
dc.subjectBiophysicsen
dc.titleSingle-molecule diffusion and conformational dynamics by spatial integration of temporal fluctuationsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalNature Communicationsen
dc.identifier.pmcidPMC4205855en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorHabuchi, Satoshien
kaust.authorBayoumi, Maged Fouaden
kaust.authorAbadi, Maramen
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