Transcriptome analysis elucidates key developmental components of bryozoan lophophore development

Handle URI:
http://hdl.handle.net/10754/333562
Title:
Transcriptome analysis elucidates key developmental components of bryozoan lophophore development
Authors:
Wong, Yue Him; Ryu, Tae Woo; Ghosheh, Yanal ( 0000-0002-3733-2098 ) ; Qian, Pei-Yuan; Seridi, Loqmane ( 0000-0001-7028-3024 ) ; Bougouffa, Salim ( 0000-0001-9218-6452 ) ; Ravasi, Timothy ( 0000-0002-9950-465X )
Abstract:
The most recent phylogenomic study suggested that Bryozoa (Ectoprocta), Brachiopoda, and Phoronida are monophyletic, implying that the lophophore of bryozoans, phoronids and brachiopods is a synapomorphy. Understanding the molecular mechanisms of the lophophore development of the Lophophorata clade can therefore provide us a new insight into the formation of the diverse morphological traits in metazoans. In the present study, we profiled the transcriptome of the Bryozoan (Ectoproct) Bugula neritina during the swimming larval stage (SW) and the early (4 h) and late (24 h) metamorphic stages using the Illumina HiSeq2000 platform. Various genes that function in development, the immune response and neurogenesis showed differential expression levels during metamorphosis. In situ hybridization of 23 genes that participate in the Wnt, BMP, Notch, and Hedgehog signaling pathways revealed their regulatory roles in the development of the lophophore and the ancestrula digestive tract. Our findings support the hypothesis that developmental precursors of the lophophore and the ancestrula digestive tract are pre-patterned by the differential expression of key developmental genes according to their fate. This study provides a foundation to better understand the developmental divergence and/or convergence among developmental precursors of the lophophore of bryozoans, branchiopods and phoronids.
KAUST Department:
Computational Bioscience Research Center (CBRC); Biological and Environmental Sciences and Engineering (BESE) Division; Applied Mathematics and Computational Science Program; Computer Science Program
Citation:
Wong, Y. H., Ryu, T., Seridi, L., Ghosheh, Y., Bougouffa, S., Qian, P.-Y., & Ravasi, T. (2014). Transcriptome analysis elucidates key developmental components of bryozoan lophophore development. Sci. Rep., 4. doi: 10.1038/srep06534
Publisher:
Nature Publishing Group
Journal:
Scientific Reports
Issue Date:
10-Oct-2014
DOI:
10.1038/srep06534
Type:
Article
ISSN:
2045-2322
Sponsors:
This study was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, an award from King Abdullah University of Science and Technology [SA-C0040/UK-C0016], and grants from the Research Grants Council of the Hong Kong Special Administrative Region [GRF661611 and GRF662413] to Pei-Yuan Qian.
Additional Links:
http://www.nature.com/doifinder/10.1038/srep06534
Appears in Collections:
Articles; Applied Mathematics and Computational Science Program; Computer Science Program; Computational Bioscience Research Center (CBRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorWong, Yue Himen
dc.contributor.authorRyu, Tae Wooen
dc.contributor.authorGhosheh, Yanalen
dc.contributor.authorQian, Pei-Yuanen
dc.contributor.authorSeridi, Loqmaneen
dc.contributor.authorBougouffa, Salimen
dc.contributor.authorRavasi, Timothyen
dc.date.accessioned2014-11-03T06:01:00Z-
dc.date.available2014-11-03T06:01:00Z-
dc.date.issued2014-10-10en
dc.identifier.citationWong, Y. H., Ryu, T., Seridi, L., Ghosheh, Y., Bougouffa, S., Qian, P.-Y., & Ravasi, T. (2014). Transcriptome analysis elucidates key developmental components of bryozoan lophophore development. Sci. Rep., 4. doi: 10.1038/srep06534en
dc.identifier.issn2045-2322en
dc.identifier.doi10.1038/srep06534en
dc.identifier.urihttp://hdl.handle.net/10754/333562en
dc.description.abstractThe most recent phylogenomic study suggested that Bryozoa (Ectoprocta), Brachiopoda, and Phoronida are monophyletic, implying that the lophophore of bryozoans, phoronids and brachiopods is a synapomorphy. Understanding the molecular mechanisms of the lophophore development of the Lophophorata clade can therefore provide us a new insight into the formation of the diverse morphological traits in metazoans. In the present study, we profiled the transcriptome of the Bryozoan (Ectoproct) Bugula neritina during the swimming larval stage (SW) and the early (4 h) and late (24 h) metamorphic stages using the Illumina HiSeq2000 platform. Various genes that function in development, the immune response and neurogenesis showed differential expression levels during metamorphosis. In situ hybridization of 23 genes that participate in the Wnt, BMP, Notch, and Hedgehog signaling pathways revealed their regulatory roles in the development of the lophophore and the ancestrula digestive tract. Our findings support the hypothesis that developmental precursors of the lophophore and the ancestrula digestive tract are pre-patterned by the differential expression of key developmental genes according to their fate. This study provides a foundation to better understand the developmental divergence and/or convergence among developmental precursors of the lophophore of bryozoans, branchiopods and phoronids.en
dc.description.sponsorshipThis study was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, an award from King Abdullah University of Science and Technology [SA-C0040/UK-C0016], and grants from the Research Grants Council of the Hong Kong Special Administrative Region [GRF661611 and GRF662413] to Pei-Yuan Qian.en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.relation.urlhttp://www.nature.com/doifinder/10.1038/srep06534en
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en
dc.subjectBiological metamorphosisen
dc.subjectTranscriptomicsen
dc.titleTranscriptome analysis elucidates key developmental components of bryozoan lophophore developmenten
dc.typeArticleen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentApplied Mathematics and Computational Science Programen
dc.contributor.departmentComputer Science Programen
dc.identifier.journalScientific Reportsen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionThe division of Life Science, School of Science, the Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kongen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorRyu, Tae Wooen
kaust.authorSeridi, Loqmaneen
kaust.authorBougouffa, Salimen
kaust.authorRavasi, Timothyen
kaust.authorGhosheh, Yanalen
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