Comparative genome analysis of three eukaryotic parasites with differing abilities to transform leukocytes reveals key mediators of theileria-induced leukocyte transformation

Handle URI:
http://hdl.handle.net/10754/325462
Title:
Comparative genome analysis of three eukaryotic parasites with differing abilities to transform leukocytes reveals key mediators of theileria-induced leukocyte transformation
Authors:
Hayashida, Kyoko; Hara, Yuichiro; Abe, Takashi; Yamasaki, Chisato; Toyoda, Atsushi; Kosuge, Takehide; Suzuki, Yutaka; Sato, Yoshiharu; Kawashima, Shuichi; Katayama, Toshiaki; Wakaguri, Hiroyuki; Inoue, Noboru; Homma, Keiichi; Tada-Umezaki, Masahito; Yagi, Yukio; Fujii, Yasuyuki; Habara, Takuya; Kanehisa, Minoru; Watanabe, Hidemi; Ito, Kimihito; Gojobori, Takashi; Sugawara, Hideaki; Imanishi, Tadashi; Weir, William; Gardner, Malcolm; Pain, Arnab ( 0000-0002-1755-2819 ) ; Shiels, Brian; Hattori, Masahira; Nene, Vishvanath; Sugimoto, Chihiro
Abstract:
We sequenced the genome of Theileria orientalis, a tick-borne apicomplexan protozoan parasite of cattle. The focus of this study was a comparative genome analysis of T. orientalis relative to other highly pathogenic Theileria species, T. parva and T. annulata. T. parva and T. annulata induce transformation of infected cells of lymphocyte or macrophage/monocyte lineages; in contrast, T. orientalis does not induce uncontrolled proliferation of infected leukocytes and multiplies predominantly within infected erythrocytes. While synteny across homologous chromosomes of the three Theileria species was found to be well conserved overall, subtelomeric structures were found to differ substantially, as T. orientalis lacks the large tandemly arrayed subtelomere-encoded variable secreted protein-encoding gene family. Moreover, expansion of particular gene families by gene duplication was found in the genomes of the two transforming Theileria species, most notably, the TashAT/TpHN and Tar/Tpr gene families. Gene families that are present only in T. parva and T. annulata and not in T. orientalis, Babesia bovis, or Plasmo-dium were also identified. Identification of differences between the genome sequences of Theileria species with different abilities to transform and immortalize bovine leukocytes will provide insight into proteins and mechanisms that have evolved to induce and regulate this process. The T. orientalis genome database is available at http://totdb.czc.hokudai.ac.jp/. 2012 Hayashida et al. T.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Computational Bioscience Research Center (CBRC); Pathogen Genomics Laboratory
Citation:
Hayashida K, Hara Y, Abe T, Yamasaki C, Toyoda A, et al. (2012) Comparative Genome Analysis of Three Eukaryotic Parasites with Differing Abilities To Transform Leukocytes Reveals Key Mediators of Theileria-Induced Leukocyte Transformation. mBio 3: e00204-12-e00204-12. doi:10.1128/mBio.00204-12.
Publisher:
American Society of Microbiology
Journal:
mBio
Issue Date:
4-Sep-2012
DOI:
10.1128/mBio.00204-12
PubMed ID:
22951932
PubMed Central ID:
PMC3445966
Type:
Article
ISSN:
21612129
Appears in Collections:
Articles; Computational Bioscience Research Center (CBRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorHayashida, Kyokoen
dc.contributor.authorHara, Yuichiroen
dc.contributor.authorAbe, Takashien
dc.contributor.authorYamasaki, Chisatoen
dc.contributor.authorToyoda, Atsushien
dc.contributor.authorKosuge, Takehideen
dc.contributor.authorSuzuki, Yutakaen
dc.contributor.authorSato, Yoshiharuen
dc.contributor.authorKawashima, Shuichien
dc.contributor.authorKatayama, Toshiakien
dc.contributor.authorWakaguri, Hiroyukien
dc.contributor.authorInoue, Noboruen
dc.contributor.authorHomma, Keiichien
dc.contributor.authorTada-Umezaki, Masahitoen
dc.contributor.authorYagi, Yukioen
dc.contributor.authorFujii, Yasuyukien
dc.contributor.authorHabara, Takuyaen
dc.contributor.authorKanehisa, Minoruen
dc.contributor.authorWatanabe, Hidemien
dc.contributor.authorIto, Kimihitoen
dc.contributor.authorGojobori, Takashien
dc.contributor.authorSugawara, Hideakien
dc.contributor.authorImanishi, Tadashien
dc.contributor.authorWeir, Williamen
dc.contributor.authorGardner, Malcolmen
dc.contributor.authorPain, Arnaben
dc.contributor.authorShiels, Brianen
dc.contributor.authorHattori, Masahiraen
dc.contributor.authorNene, Vishvanathen
dc.contributor.authorSugimoto, Chihiroen
dc.date.accessioned2014-08-27T09:52:27Z-
dc.date.available2014-08-27T09:52:27Z-
dc.date.issued2012-09-04en
dc.identifier.citationHayashida K, Hara Y, Abe T, Yamasaki C, Toyoda A, et al. (2012) Comparative Genome Analysis of Three Eukaryotic Parasites with Differing Abilities To Transform Leukocytes Reveals Key Mediators of Theileria-Induced Leukocyte Transformation. mBio 3: e00204-12-e00204-12. doi:10.1128/mBio.00204-12.en
dc.identifier.issn21612129en
dc.identifier.pmid22951932en
dc.identifier.doi10.1128/mBio.00204-12en
dc.identifier.urihttp://hdl.handle.net/10754/325462en
dc.description.abstractWe sequenced the genome of Theileria orientalis, a tick-borne apicomplexan protozoan parasite of cattle. The focus of this study was a comparative genome analysis of T. orientalis relative to other highly pathogenic Theileria species, T. parva and T. annulata. T. parva and T. annulata induce transformation of infected cells of lymphocyte or macrophage/monocyte lineages; in contrast, T. orientalis does not induce uncontrolled proliferation of infected leukocytes and multiplies predominantly within infected erythrocytes. While synteny across homologous chromosomes of the three Theileria species was found to be well conserved overall, subtelomeric structures were found to differ substantially, as T. orientalis lacks the large tandemly arrayed subtelomere-encoded variable secreted protein-encoding gene family. Moreover, expansion of particular gene families by gene duplication was found in the genomes of the two transforming Theileria species, most notably, the TashAT/TpHN and Tar/Tpr gene families. Gene families that are present only in T. parva and T. annulata and not in T. orientalis, Babesia bovis, or Plasmo-dium were also identified. Identification of differences between the genome sequences of Theileria species with different abilities to transform and immortalize bovine leukocytes will provide insight into proteins and mechanisms that have evolved to induce and regulate this process. The T. orientalis genome database is available at http://totdb.czc.hokudai.ac.jp/. 2012 Hayashida et al. T.en
dc.language.isoenen
dc.publisherAmerican Society of Microbiologyen
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en
dc.subjectBabesia bovisen
dc.subjectcell immortalizationen
dc.subjectcell lineageen
dc.subjectcell proliferationen
dc.subjectchromosome structureen
dc.subjectcontrolled studyen
dc.subjecterythrocyteen
dc.subjecteukaryoteen
dc.subjectgene duplicationen
dc.subjectgenetic codeen
dc.subjectgenetic conservationen
dc.subjectgenome analysisen
dc.subjectleukocyteen
dc.subjectlymphocyteen
dc.subjectlymphocyte transformationen
dc.subjectmacrophageen
dc.subjectmonocyteen
dc.subjectmultigene familyen
dc.subjectnucleotide sequenceen
dc.subjectparasite identificationen
dc.subjectPlasmodiumen
dc.subjectregulatory mechanismen
dc.subjectsequence analysisen
dc.subjectspecies comparisonen
dc.subjectspecies differentiationen
dc.subjectsyntenyen
dc.subjecttelomereen
dc.subjectTheileriaen
dc.subjectTheileria annulataen
dc.subjectTheileria orientalisen
dc.subjectTheileria parvaen
dc.subjectunindexed sequenceen
dc.subjectBabesia bovisen
dc.subjectBosen
dc.subjectBovinaeen
dc.subjectEukaryotaen
dc.subjectIxodidaen
dc.subjectProtozoaen
dc.subjectTheileriaen
dc.subjectTheileria annulataen
dc.subjectTheileria orientalisen
dc.subjectTheileria parvaen
dc.titleComparative genome analysis of three eukaryotic parasites with differing abilities to transform leukocytes reveals key mediators of theileria-induced leukocyte transformationen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.contributor.departmentPathogen Genomics Laboratoryen
dc.identifier.journalmBioen
dc.identifier.pmcidPMC3445966en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDivision of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido, Japanen
dc.contributor.institutionBiomedicinal Information Research Center, National Institute of Advanced Industrial Science and Technology, Tokyo, Japanen
dc.contributor.institutionInformation Engineering, Niigata University, Niigata, Japanen
dc.contributor.institutionComparative Genomics Laboratory, Center for Genetic Resource Information, National Institute of Genetics, Research Organization of Information and Systems, Mishima, Shizuoka, Japanen
dc.contributor.institutionCenter for Information Biology and DNA Data Bank of Japan, National Institute of Genetics, Mishima, Shizuoka, Japanen
dc.contributor.institutionDepartment of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, Tokyo, Japanen
dc.contributor.institutionGraduate School of Pharmaceutical Sciences, Chiba University, Chiba, Japanen
dc.contributor.institutionHuman Genome Center, Institute of Medical Science, University of Tokyo, Tokyo, Japanen
dc.contributor.institutionNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japanen
dc.contributor.institutionInstitute of Natural Medicine, University of Toyama, Toyama, Japanen
dc.contributor.institutionHokkaido Research Station, National Institute of Animal Health, National Agricultural Research Organization, Sapporo, Hokkaido, Japanen
dc.contributor.institutionOkayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japanen
dc.contributor.institutionGraduate School of Information Science and Technology, Hokkaido University, Sapporo, Hokkaido, Japanen
dc.contributor.institutionDivision of Bioinformatics, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido, Japanen
dc.contributor.institutionInstitute of Comparative Medicine, Glasgow University Veterinary School, Glasgow, United Kingdomen
dc.contributor.institutionSeattle Biomedical Research Institute, Seattle, WA, United Statesen
dc.contributor.institutionInternational Livestock Research Institute, Nairobi, Kenyaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorPain, Arnaben

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