Dissecting the interactions of SERRATE with RNA and DICER-LIKE 1 in Arabidopsis microRNA precursor processing

Handle URI:
http://hdl.handle.net/10754/325455
Title:
Dissecting the interactions of SERRATE with RNA and DICER-LIKE 1 in Arabidopsis microRNA precursor processing
Authors:
Iwata, Yuji; Takahashi, Masateru; Fedoroff, Nina V.; Hamdan, Samir ( 0000-0001-5192-1852 )
Abstract:
Efficient and precise microRNA (miRNA) biogenesis in Arabidopsis is mediated by the RNaseIII-family enzyme DICER-LIKE 1 (DCL1), double-stranded RNA-binding protein HYPONASTIC LEAVES 1 and the zinc-finger (ZnF) domain-containing protein SERRATE (SE). In the present study, we examined primary miRNA precursor (pri-miRNA) processing by highly purified recombinant DCL1 and SE proteins and found that SE is integral to pri-miRNA processing by DCL1. SE stimulates DCL1 cleavage of the pri-miRNA in an ionic strength-dependent manner. SE uses its N-terminal domain to bind to RNA and requires both N-terminal and ZnF domains to bind to DCL1. However, when DCL1 is bound to RNA, the interaction with the ZnF domain of SE becomes indispensible and stimulates the activity of DCL1 without requiring SE binding to RNA. Our results suggest that the interactions among SE, DCL1 and RNA are a potential point for regulating pri-miRNA processing. 2013 The Author(s) 2013.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Center for Desert Agriculture
Citation:
Iwata Y, Takahashi M, Fedoroff NV, Hamdan SM (2013) Dissecting the interactions of SERRATE with RNA and DICER-LIKE 1 in Arabidopsis microRNA precursor processing. Nucleic Acids Research 41: 9129-9140. doi:10.1093/nar/gkt667.
Publisher:
Oxford University Press (OUP)
Journal:
Nucleic Acids Research
Issue Date:
5-Aug-2013
DOI:
10.1093/nar/gkt667
PubMed ID:
23921632
PubMed Central ID:
PMC3799435
Type:
Article
ISSN:
03051048
Appears in Collections:
Articles; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorIwata, Yujien
dc.contributor.authorTakahashi, Masateruen
dc.contributor.authorFedoroff, Nina V.en
dc.contributor.authorHamdan, Samiren
dc.date.accessioned2014-08-27T09:52:03Z-
dc.date.available2014-08-27T09:52:03Z-
dc.date.issued2013-08-05en
dc.identifier.citationIwata Y, Takahashi M, Fedoroff NV, Hamdan SM (2013) Dissecting the interactions of SERRATE with RNA and DICER-LIKE 1 in Arabidopsis microRNA precursor processing. Nucleic Acids Research 41: 9129-9140. doi:10.1093/nar/gkt667.en
dc.identifier.issn03051048en
dc.identifier.pmid23921632en
dc.identifier.doi10.1093/nar/gkt667en
dc.identifier.urihttp://hdl.handle.net/10754/325455en
dc.description.abstractEfficient and precise microRNA (miRNA) biogenesis in Arabidopsis is mediated by the RNaseIII-family enzyme DICER-LIKE 1 (DCL1), double-stranded RNA-binding protein HYPONASTIC LEAVES 1 and the zinc-finger (ZnF) domain-containing protein SERRATE (SE). In the present study, we examined primary miRNA precursor (pri-miRNA) processing by highly purified recombinant DCL1 and SE proteins and found that SE is integral to pri-miRNA processing by DCL1. SE stimulates DCL1 cleavage of the pri-miRNA in an ionic strength-dependent manner. SE uses its N-terminal domain to bind to RNA and requires both N-terminal and ZnF domains to bind to DCL1. However, when DCL1 is bound to RNA, the interaction with the ZnF domain of SE becomes indispensible and stimulates the activity of DCL1 without requiring SE binding to RNA. Our results suggest that the interactions among SE, DCL1 and RNA are a potential point for regulating pri-miRNA processing. 2013 The Author(s) 2013.en
dc.language.isoenen
dc.publisherOxford University Press (OUP)en
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/en
dc.subjectdiceren
dc.subjectdicer like 1 proteinen
dc.subjectJagged1en
dc.subjectmicroRNAen
dc.subjectrecombinant proteinen
dc.subjectRNAen
dc.subjectunclassified drugen
dc.subjectzinc finger proteinen
dc.subjectamino terminal sequenceen
dc.subjectArabidopsisen
dc.subjectbaculovirus expression systemen
dc.subjectin vitro studyen
dc.subjectinsect cellen
dc.subjectionic strengthen
dc.subjectprocess optimizationen
dc.subjectprotein domainen
dc.subjectprotein expressionen
dc.subjectprotein functionen
dc.subjectprotein protein interactionen
dc.subjectprotein purificationen
dc.subjectRNA bindingen
dc.subjectRNA cleavageen
dc.subjectRNA processingen
dc.subjectRNA splicingen
dc.subjectArabidopsisen
dc.subjectArabidopsis Proteinsen
dc.subjectCalcium-Binding Proteinsen
dc.subjectCell Cycle Proteinsen
dc.subjectGene Expression Regulation, Planten
dc.subjectIntercellular Signaling Peptides and Proteinsen
dc.subjectMembrane Proteinsen
dc.subjectMicroRNAsen
dc.subjectOsmolar Concentrationen
dc.subjectProtein Structure, Tertiaryen
dc.subjectRibonuclease IIIen
dc.subjectRNA Precursorsen
dc.subjectRNA Processing, Post-Transcriptionalen
dc.subjectSequence Deletionen
dc.subjectSf9 Cellsen
dc.subjectSpodopteraen
dc.titleDissecting the interactions of SERRATE with RNA and DICER-LIKE 1 in Arabidopsis microRNA precursor processingen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalNucleic Acids Researchen
dc.identifier.pmcidPMC3799435en
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionGraduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japanen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorIwata, Yujien
kaust.authorTakahashi, Masateruen
kaust.authorFedoroff, Nina V.en
kaust.authorHamdan, Samiren

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